A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s...A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.展开更多
Background： Recently, deer have expanded their distribution to higher altitude ranges including subalpine forests However, culling deer and construction of deer fence in subalpine forests are difficult because of ste...Background： Recently, deer have expanded their distribution to higher altitude ranges including subalpine forests However, culling deer and construction of deer fence in subalpine forests are difficult because of steep slopes and complex topography. Thus it is necessary to clarify the factors which are associated with debarking by deer for the effective protection of subalpine forests. In this study, we examined which factors are associated with debarking b： sika deer （Cervus nippon） in subalpine coniferous forests. Methods： We conducted our survey in Minami-Alps National Park, central Japan. We established 24 10 m × 40 m plots and surveyed the occurrence of debarking on saplings 〉30 cm in height and 〈3 cm in diameter at breast heigh （DBH） and on trees 〉3 cm in DBH, as well as sapling density within each plot. Minimum distances to nearest grasslan（ of plots were calculated （tentatively assuming grassland would attract deer and would cause high debarking pressure in the surrounding subaipine forests）. Results： The mean percentage of debarked live saplings was higher than that of live trees. The mean percentage of debarked saplings which had already died was 81.6 %. Debarking of saplings increased with lower elevation taller sapling size, and marginally increased near grassland. Sapling density was lower in plots with low basa area of conspecific trees near grassland and differed among species. Sapling density marginally decreased with decreasing elevation and increasing stand tree density. Debarking of trees was positively related to small DBH and Io~ elevation, and marginally increased near grassland and differed among species. Conclusions： Our results suggest that tall saplings in subalpine forests of low elevation or near subalpine grassland were susceptible to debarking by deer and monitoring of these areas may permit the early detection of the impacts of deer in subalpine coniferous forests.展开更多
文摘A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.
基金funded by the Comprehensive Research Organization for Science and Technology of Yamanashi Prefectural Government and Mitsui&CO.,LTD
文摘Background： Recently, deer have expanded their distribution to higher altitude ranges including subalpine forests However, culling deer and construction of deer fence in subalpine forests are difficult because of steep slopes and complex topography. Thus it is necessary to clarify the factors which are associated with debarking by deer for the effective protection of subalpine forests. In this study, we examined which factors are associated with debarking b： sika deer （Cervus nippon） in subalpine coniferous forests. Methods： We conducted our survey in Minami-Alps National Park, central Japan. We established 24 10 m × 40 m plots and surveyed the occurrence of debarking on saplings 〉30 cm in height and 〈3 cm in diameter at breast heigh （DBH） and on trees 〉3 cm in DBH, as well as sapling density within each plot. Minimum distances to nearest grasslan（ of plots were calculated （tentatively assuming grassland would attract deer and would cause high debarking pressure in the surrounding subaipine forests）. Results： The mean percentage of debarked live saplings was higher than that of live trees. The mean percentage of debarked saplings which had already died was 81.6 %. Debarking of saplings increased with lower elevation taller sapling size, and marginally increased near grassland. Sapling density was lower in plots with low basa area of conspecific trees near grassland and differed among species. Sapling density marginally decreased with decreasing elevation and increasing stand tree density. Debarking of trees was positively related to small DBH and Io~ elevation, and marginally increased near grassland and differed among species. Conclusions： Our results suggest that tall saplings in subalpine forests of low elevation or near subalpine grassland were susceptible to debarking by deer and monitoring of these areas may permit the early detection of the impacts of deer in subalpine coniferous forests.
