A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H202. This finding leads to a novel approa...A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H202. This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx) which, on oxidation of ethanol, produces H202. The method has higher sensitivity, wider analytical range (0.1-8 mmol/L), and a lower detection limit (0.05 mmol/L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.展开更多
基金Project(21005067)supported by the National Natural Science Foundation of ChinaProject(11JJ4015)supported by Natural Science Foundation of Hunan Province,China
文摘A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H202. This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx) which, on oxidation of ethanol, produces H202. The method has higher sensitivity, wider analytical range (0.1-8 mmol/L), and a lower detection limit (0.05 mmol/L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.