Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could f...Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.展开更多
Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of ...Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.展开更多
为建立适宜的仙客来耐热评价体系并筛选耐热性种质,本研究选取20个主栽仙客来品种,进行每日4 h 40℃高温胁迫处理,在胁迫0、5、10、15 d后系统测定形态与生理生化指标,通过相关性分析、隶属函数分析、聚类分析和逐步回归分析及多指标建...为建立适宜的仙客来耐热评价体系并筛选耐热性种质,本研究选取20个主栽仙客来品种,进行每日4 h 40℃高温胁迫处理,在胁迫0、5、10、15 d后系统测定形态与生理生化指标,通过相关性分析、隶属函数分析、聚类分析和逐步回归分析及多指标建模等方法综合评价品种耐热性。结果表明:高温胁迫显著改变植株表型及生理参数,品种间响应模式存在显著差异(P<0.05);隶属函数分析显示耐热性综合评价值(D值)变异范围为0.271~0.695,其中CP16(D=0.695)耐热性最优,CP6(D=0.271)最敏感;聚类分析将供试品种分为3类:耐热型(CP16)、中间型(CP2、CP3、CP7等13个品种)及敏感型(CP1、CP4、CP5、CP6、CP18、CP20);通过多元回归分析,建立了仙客来品种的耐热性评价模型,筛选出5个评价指标(叶宽、叶片含水率、Pro、SOD、Fv/Fo)。本研究建立的评价体系为仙客来耐热品种选育提供了理论依据与技术支撑。展开更多
在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其...在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其中1.0 m g/L TDZ对叶片再生芽的诱导效果最好,而子叶以0.5 m g/LTDZ、子叶节和叶柄以0.2 m g/L TDZ诱导再生芽的效果较好。展开更多
文摘Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.
文摘Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.
文摘为建立适宜的仙客来耐热评价体系并筛选耐热性种质,本研究选取20个主栽仙客来品种,进行每日4 h 40℃高温胁迫处理,在胁迫0、5、10、15 d后系统测定形态与生理生化指标,通过相关性分析、隶属函数分析、聚类分析和逐步回归分析及多指标建模等方法综合评价品种耐热性。结果表明:高温胁迫显著改变植株表型及生理参数,品种间响应模式存在显著差异(P<0.05);隶属函数分析显示耐热性综合评价值(D值)变异范围为0.271~0.695,其中CP16(D=0.695)耐热性最优,CP6(D=0.271)最敏感;聚类分析将供试品种分为3类:耐热型(CP16)、中间型(CP2、CP3、CP7等13个品种)及敏感型(CP1、CP4、CP5、CP6、CP18、CP20);通过多元回归分析,建立了仙客来品种的耐热性评价模型,筛选出5个评价指标(叶宽、叶片含水率、Pro、SOD、Fv/Fo)。本研究建立的评价体系为仙客来耐热品种选育提供了理论依据与技术支撑。
文摘在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其中1.0 m g/L TDZ对叶片再生芽的诱导效果最好,而子叶以0.5 m g/LTDZ、子叶节和叶柄以0.2 m g/L TDZ诱导再生芽的效果较好。
