OBJECTIVE To investigate the effects of IMM-H004 on permanent focal cerebral ischemia injury and associated cardiopulmonary complications,further elucidating the molecular mechanisms.METHODS The effects of IMM-H004 we...OBJECTIVE To investigate the effects of IMM-H004 on permanent focal cerebral ischemia injury and associated cardiopulmonary complications,further elucidating the molecular mechanisms.METHODS The effects of IMM-H004 were investigated in wild-type(WT) and CKLF1-/-rats.The effects of IMM-H004 on ischemic stroke injury and its cardiopulmonary complications were determined using 2,3,5-triphenyltetrazolium chloride(TTC) staining,behavior tests,magnetic resonance imaging(MRI)scans,enzyme-linked immunosorbent assay(ELISA),Nissl staining,and histo-pathological examination.Multiple molecular experiments including immunohistological staining,immunofluorescence staining,quantitative RT-PCR,Western blotting,and co-immunoprecipitation assays were used to elucidate the underlying mechanisms.RESULTS IMM-H004 treatment provided significant protection against ischemic stroke-induced brain injury and associated cardiopulmonary complications,through CKLF1-depedent-anti-inflammation pathway in rats.IMM-H004 downregulated the amount of CKLF1 and disturbed the combination between CKLF1 and C-C chemokine receptor type 4,suppressing the inflammatory response and protecting the damaged organs in ischemic setting.CONCLUSION This preclinical study established efficacy of IMM-H004 as a potential therapeutic medicine for ischemic stroke and associated cardiopulmonary complications.The protective effects of IMM-H004 may due to its specific mechanism through CKLF1.These results support further efforts to develop IMM-H004 for human clinical trials in acute cerebral ischemia,especially for patients who are not suitable for reperfusion therapy.展开更多
Background and Objective Ablation within the pulmonary sinus of Valsalva (PSV) becomes increasingly common in certain ventricular outflow arrhythmia. Understanding the regional anatomy is intensively concerned to avoi...Background and Objective Ablation within the pulmonary sinus of Valsalva (PSV) becomes increasingly common in certain ventricular outflow arrhythmia. Understanding the regional anatomy is intensively concerned to avoid procedure complications. The purpose of this study is to describe the anatomic relationships of PSV to its adjacent structures using computed tomographic coronary angiograms (CTCA).展开更多
OBJECTIVE To explore whether J24924could prevent the development of pristane-induced lupus in a mouse model,and whether it could protect renal and lower the cardiovascular risk.METHODS The effect of J24924 was assesse...OBJECTIVE To explore whether J24924could prevent the development of pristane-induced lupus in a mouse model,and whether it could protect renal and lower the cardiovascular risk.METHODS The effect of J24924 was assessed in female BALB/c mice intraperitoneal injected with 0.5 m L of pristane,and serum autoantibodies were tested every month,blood pressure wasmeasured every 2 months,while serum inflammatory markers,spleen pathologic characteristics,renal injury and vascular function were observed at 6 month.RESULTS J24924 could decrease serum autoantibodies and serum inflammatory markers in the SLE mice and improved the spleen pathologic characteristics,and at the same time improved the renal injury and decreased inflammatory responses in kidneys,reduced blood pressure and improved vascular endothelial function.Western blotting assays revealed that inhibition for the activation of NF-κB and Rho/ROCKs signaling pathways and the downstream signaling molecules might be the potential mechanisms of J24924.CONCLUSION Our findings suggestthat therapy of J24924 may be a strategy to prevent SLE and ameliorate associated kidney and cardiovascular complications.展开更多
In order to increase productivity and reduce energy consumption of steelmaking-continuous casting(SCC) production process, especially with complicated technological routes, the cross entropy(CE) method was adopted to ...In order to increase productivity and reduce energy consumption of steelmaking-continuous casting(SCC) production process, especially with complicated technological routes, the cross entropy(CE) method was adopted to optimize the SCC production scheduling(SCCPS) problem. Based on the CE method, a matrix encoding scheme was proposed and a backward decoding method was used to generate a reasonable schedule. To describe the distribution of the solution space, a probability distribution model was built and used to generate individuals. In addition, the probability updating mechanism of the probability distribution model was proposed which helps to find the optimal individual gradually. Because of the poor stability and premature convergence of the standard cross entropy(SCE) algorithm, the improved cross entropy(ICE) algorithm was proposed with the following improvements: individual generation mechanism combined with heuristic rules, retention mechanism of the optimal individual, local search mechanism and dynamic parameters of the algorithm. Simulation experiments validate that the CE method is effective in solving the SCCPS problem with complicated technological routes and the ICE algorithm proposed has superior performance to the SCE algorithm and the genetic algorithm(GA).展开更多
The fatigue life of aeroengine turbine disc presents great dispersion due to the randomness of the basic variables,such as applied load,working temperature,geometrical dimensions and material properties.In order to am...The fatigue life of aeroengine turbine disc presents great dispersion due to the randomness of the basic variables,such as applied load,working temperature,geometrical dimensions and material properties.In order to ameliorate reliability analysis efficiency without loss of reliability,the distributed collaborative response surface method(DCRSM) was proposed,and its basic theories were established in this work.Considering the failure dependency among the failure modes,the distributed response surface was constructed to establish the relationship between the failure mode and the relevant random variables.Then,the failure modes were considered as the random variables of system response to obtain the distributed collaborative response surface model based on structure failure criterion.Finally,the given turbine disc structure was employed to illustrate the feasibility and validity of the presented method.Through the comparison of DCRSM,Monte Carlo method(MCM) and the traditional response surface method(RSM),the results show that the computational precision for DCRSM is more consistent with MCM than RSM,while DCRSM needs far less computing time than MCM and RSM under the same simulation conditions.Thus,DCRSM is demonstrated to be a feasible and valid approach for improving the computational efficiency of reliability analysis for aeroengine turbine disc fatigue life with multiple random variables,and has great potential value for the complicated mechanical structure with multi-component and multi-failure mode.展开更多
OBJECTIVE To investigate the potency of Urena lobata leaves extract on the inhibition of hepatic complication on diabetic rats.METHODS This study uses control group post test only with male Sprague dawley rats.Diabeti...OBJECTIVE To investigate the potency of Urena lobata leaves extract on the inhibition of hepatic complication on diabetic rats.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in concentrations of 250,500 and 1000mg·kg-1 bw for 4 weeks.After scarifying,liver organ and blood were collected and then superoxyde dismutase(SOD)hepar level,malondialdehyda(MDA),tumor necrosis factor-alpha(TNF-α),serum glutamic oxaloacetic transaminase(SGOT)and serum glutamic piruvic transaminase(SGPT)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0,05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD hepar level about 90%,100% and 120%respectively compared to diabetic group(P<0.05),while the MDA hepar level was decreased by 40%,50% and 70% respectively(P<0.05),whereas the TNF-αhepar level was decreased by 30%,50% and 70%respectively(P<0.05).The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw decrease SGOT level approximately10%,30% and 50% compared to control group(P<0.05),while the SGPT level was decreased by 10%,20% and 40%respectively(P<0.05).In diabetic groups,SOD heparlevel was decreased compared to normal group(P<0.05)whereas the MDA and TNF-αwere increased(P<0.05).Meanwhile SGOT level and SGPT were increased in diabetic group(P<0.05).CONCLUSION U.lobataleaves extract could inhibit hepatic complication on diabetic rats by increasing of SOD hepar level,decreasing of MDA hepar level,TNF-α,SGOT and SGPT.This effect may be related to active compounds that act as an antioxidant and anti-inflammatory in U.lobata extract.展开更多
基金The project supported by ZYBZH-Y-HUN-24National Natural Science Foundation of China(81730096+6 种基金U1402221)National Mega-project for Innovative Drugs(2018ZX09711001-002-0072018ZX09711001-003-0052018ZX09711001-009-013)CAMS Innovation Fund for Medical Sciences(2016-I2M-1-004)Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study(BZ0150)PUMC Graduate Educationand Teaching Reform Project(10023201600801)
文摘OBJECTIVE To investigate the effects of IMM-H004 on permanent focal cerebral ischemia injury and associated cardiopulmonary complications,further elucidating the molecular mechanisms.METHODS The effects of IMM-H004 were investigated in wild-type(WT) and CKLF1-/-rats.The effects of IMM-H004 on ischemic stroke injury and its cardiopulmonary complications were determined using 2,3,5-triphenyltetrazolium chloride(TTC) staining,behavior tests,magnetic resonance imaging(MRI)scans,enzyme-linked immunosorbent assay(ELISA),Nissl staining,and histo-pathological examination.Multiple molecular experiments including immunohistological staining,immunofluorescence staining,quantitative RT-PCR,Western blotting,and co-immunoprecipitation assays were used to elucidate the underlying mechanisms.RESULTS IMM-H004 treatment provided significant protection against ischemic stroke-induced brain injury and associated cardiopulmonary complications,through CKLF1-depedent-anti-inflammation pathway in rats.IMM-H004 downregulated the amount of CKLF1 and disturbed the combination between CKLF1 and C-C chemokine receptor type 4,suppressing the inflammatory response and protecting the damaged organs in ischemic setting.CONCLUSION This preclinical study established efficacy of IMM-H004 as a potential therapeutic medicine for ischemic stroke and associated cardiopulmonary complications.The protective effects of IMM-H004 may due to its specific mechanism through CKLF1.These results support further efforts to develop IMM-H004 for human clinical trials in acute cerebral ischemia,especially for patients who are not suitable for reperfusion therapy.
文摘Background and Objective Ablation within the pulmonary sinus of Valsalva (PSV) becomes increasingly common in certain ventricular outflow arrhythmia. Understanding the regional anatomy is intensively concerned to avoid procedure complications. The purpose of this study is to describe the anatomic relationships of PSV to its adjacent structures using computed tomographic coronary angiograms (CTCA).
基金The project supported by National Science and Technology Major Project(2013ZX09103001-008,2013ZX09402203)the National Natural Science Foundation of China(81573645)
文摘OBJECTIVE To explore whether J24924could prevent the development of pristane-induced lupus in a mouse model,and whether it could protect renal and lower the cardiovascular risk.METHODS The effect of J24924 was assessed in female BALB/c mice intraperitoneal injected with 0.5 m L of pristane,and serum autoantibodies were tested every month,blood pressure wasmeasured every 2 months,while serum inflammatory markers,spleen pathologic characteristics,renal injury and vascular function were observed at 6 month.RESULTS J24924 could decrease serum autoantibodies and serum inflammatory markers in the SLE mice and improved the spleen pathologic characteristics,and at the same time improved the renal injury and decreased inflammatory responses in kidneys,reduced blood pressure and improved vascular endothelial function.Western blotting assays revealed that inhibition for the activation of NF-κB and Rho/ROCKs signaling pathways and the downstream signaling molecules might be the potential mechanisms of J24924.CONCLUSION Our findings suggestthat therapy of J24924 may be a strategy to prevent SLE and ameliorate associated kidney and cardiovascular complications.
基金Project(ZR2014FM036)supported by Shandong Provincial Natural Science Foundation of ChinaProject(ZR2010FZ001)supported by the Key Program of Shandong Provincial Natural Science Foundation of China
文摘In order to increase productivity and reduce energy consumption of steelmaking-continuous casting(SCC) production process, especially with complicated technological routes, the cross entropy(CE) method was adopted to optimize the SCC production scheduling(SCCPS) problem. Based on the CE method, a matrix encoding scheme was proposed and a backward decoding method was used to generate a reasonable schedule. To describe the distribution of the solution space, a probability distribution model was built and used to generate individuals. In addition, the probability updating mechanism of the probability distribution model was proposed which helps to find the optimal individual gradually. Because of the poor stability and premature convergence of the standard cross entropy(SCE) algorithm, the improved cross entropy(ICE) algorithm was proposed with the following improvements: individual generation mechanism combined with heuristic rules, retention mechanism of the optimal individual, local search mechanism and dynamic parameters of the algorithm. Simulation experiments validate that the CE method is effective in solving the SCCPS problem with complicated technological routes and the ICE algorithm proposed has superior performance to the SCE algorithm and the genetic algorithm(GA).
基金Project(51335003)supported by the National Natural Science Foundation of ChinaProject(20111102110011)supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China
文摘The fatigue life of aeroengine turbine disc presents great dispersion due to the randomness of the basic variables,such as applied load,working temperature,geometrical dimensions and material properties.In order to ameliorate reliability analysis efficiency without loss of reliability,the distributed collaborative response surface method(DCRSM) was proposed,and its basic theories were established in this work.Considering the failure dependency among the failure modes,the distributed response surface was constructed to establish the relationship between the failure mode and the relevant random variables.Then,the failure modes were considered as the random variables of system response to obtain the distributed collaborative response surface model based on structure failure criterion.Finally,the given turbine disc structure was employed to illustrate the feasibility and validity of the presented method.Through the comparison of DCRSM,Monte Carlo method(MCM) and the traditional response surface method(RSM),the results show that the computational precision for DCRSM is more consistent with MCM than RSM,while DCRSM needs far less computing time than MCM and RSM under the same simulation conditions.Thus,DCRSM is demonstrated to be a feasible and valid approach for improving the computational efficiency of reliability analysis for aeroengine turbine disc fatigue life with multiple random variables,and has great potential value for the complicated mechanical structure with multi-component and multi-failure mode.
基金The project supported by Ministry Education of Indonesia
文摘OBJECTIVE To investigate the potency of Urena lobata leaves extract on the inhibition of hepatic complication on diabetic rats.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in concentrations of 250,500 and 1000mg·kg-1 bw for 4 weeks.After scarifying,liver organ and blood were collected and then superoxyde dismutase(SOD)hepar level,malondialdehyda(MDA),tumor necrosis factor-alpha(TNF-α),serum glutamic oxaloacetic transaminase(SGOT)and serum glutamic piruvic transaminase(SGPT)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0,05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD hepar level about 90%,100% and 120%respectively compared to diabetic group(P<0.05),while the MDA hepar level was decreased by 40%,50% and 70% respectively(P<0.05),whereas the TNF-αhepar level was decreased by 30%,50% and 70%respectively(P<0.05).The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw decrease SGOT level approximately10%,30% and 50% compared to control group(P<0.05),while the SGPT level was decreased by 10%,20% and 40%respectively(P<0.05).In diabetic groups,SOD heparlevel was decreased compared to normal group(P<0.05)whereas the MDA and TNF-αwere increased(P<0.05).Meanwhile SGOT level and SGPT were increased in diabetic group(P<0.05).CONCLUSION U.lobataleaves extract could inhibit hepatic complication on diabetic rats by increasing of SOD hepar level,decreasing of MDA hepar level,TNF-α,SGOT and SGPT.This effect may be related to active compounds that act as an antioxidant and anti-inflammatory in U.lobata extract.
