A new technique, involving tool making, clearing, staining, cutting and mounting specimens on slides, was described for studying Collembola taxonomy. It could resolve the problem of observing Collembola glass specimen...A new technique, involving tool making, clearing, staining, cutting and mounting specimens on slides, was described for studying Collembola taxonomy. It could resolve the problem of observing Collembola glass specimens under standard optical microscopy, because the phase-contrast microscope has not been available for all researchers. A type of micro cutting probe （about 1 μm in tip diameter） was designed, and it made the cutting method go from two-handed to one-handed, which was a huge step forward in the cutting method of Collembola. The micro cutting probe was custom designed for Collembola using two electric grinders, followed by ferric tannate staining with a stable blue color, mounting in specialized solutions and sealing with a neutral quick-drying gum. We also described a method to make glass capillary extractor and glass capillary brush to make sure a better condition of cleared specimens.展开更多
为了研究海拔大尺度变化条件下土壤跳虫结构及多样性的变化动态,于2014年7月和9月对贡嘎山东坡1600、2000、2400、2800、3200 m 5个不同海拔梯度土壤跳虫群落进行了调查,共获得土壤跳虫个体2647只,平均密度为6925 ind.m^(-2),分属于4目1...为了研究海拔大尺度变化条件下土壤跳虫结构及多样性的变化动态,于2014年7月和9月对贡嘎山东坡1600、2000、2400、2800、3200 m 5个不同海拔梯度土壤跳虫群落进行了调查,共获得土壤跳虫个体2647只,平均密度为6925 ind.m^(-2),分属于4目10科29属,其中符(?)属(Folsomia)为优势类群,占总捕获个体数的59.9%。PCA分析结果表明,海拔3200和1600 m生境土壤跳虫群落结构差异最明显,其余海拔梯度间的群落差异相对较小。土壤跳虫类群数、密度和Shannon-Wiener指数随海拔升高总体上呈上升趋势,而优势度指数变化趋势相反,其中Shannon-Wiener指数和优势度指数变化具有显著差异。月份的变化仅对海拔3200 m土壤跳虫密度有显著影响。多元回归分析结果表明,土壤pH值、全钾含量和速效氮含量是影响土壤弹尾虫群落多样性的主要因子。展开更多
文摘A new technique, involving tool making, clearing, staining, cutting and mounting specimens on slides, was described for studying Collembola taxonomy. It could resolve the problem of observing Collembola glass specimens under standard optical microscopy, because the phase-contrast microscope has not been available for all researchers. A type of micro cutting probe （about 1 μm in tip diameter） was designed, and it made the cutting method go from two-handed to one-handed, which was a huge step forward in the cutting method of Collembola. The micro cutting probe was custom designed for Collembola using two electric grinders, followed by ferric tannate staining with a stable blue color, mounting in specialized solutions and sealing with a neutral quick-drying gum. We also described a method to make glass capillary extractor and glass capillary brush to make sure a better condition of cleared specimens.
