目的:分析CD4^(+)T淋巴细胞计数和病毒载量与艾滋病患者机会性感染的相关性,为预防和治疗艾滋病患者的机会性感染提供参考。方法:选取2023年1月—12月现住址为吉安市的1377例接受抗艾滋病治疗的艾滋病患者作为研究对象,按照是否发生机...目的:分析CD4^(+)T淋巴细胞计数和病毒载量与艾滋病患者机会性感染的相关性,为预防和治疗艾滋病患者的机会性感染提供参考。方法:选取2023年1月—12月现住址为吉安市的1377例接受抗艾滋病治疗的艾滋病患者作为研究对象,按照是否发生机会性感染分为感染组与未感染组,采集所有艾滋病患者的年龄、性别、婚姻状况、文化程度、感染途径、临床分期等基本临床信息,以及血中CD4^(+)T淋巴细胞计数和病毒载量等指标的水平,分析机会性感染的发生与CD4^(+)T淋巴细胞计数、病毒载量的关系。结果:1377例接受抗病毒治疗的艾滋病患者中发生机会性感染的有130例(感染发生率为9.44%),感染主要累及呼吸系统(55例,占42.31%),其次为皮肤黏膜(25例,占19.23%);机会性感染患者的CD4^(+)T淋巴细胞计数水平显著低于非机会性感染患者(145.28±56.39 vs 211.25±57.10,P<0.05),而其病毒载量>104/mL的比例则明显高于非机会性感染患者(76.15%vs22.05%,P<0.05);Spearman相关分析结果显示,艾滋病患者发生机会性感染与病毒载量呈正相关(r=0.356,P<0.001),而与CD4^(+)T淋巴细胞计数呈负相关(r=-0.321,P<0.001)。结论:艾滋病患者发生机会性感染的概率较高,而CD4^(+)T淋巴细胞计数的下降和病毒载量的上升会增加机会性感染的发生风险,临床应加强对风险患者的管理和照护,以减少或及早发现机会性感染的发生。展开更多
BACKGROUND： The effect of pituitary adenylate cyclase activating polypeptide （PACAP） during traumatic brain injury （TBI） and whether it can modulate secondary injury has not been reported previously. The present ...BACKGROUND： The effect of pituitary adenylate cyclase activating polypeptide （PACAP） during traumatic brain injury （TBI） and whether it can modulate secondary injury has not been reported previously. The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.METHODS： Male Sprague Dawley rats were randomly divided into 3 treatment groups （n=6, each）： sham-operated, vehicle （normal saline）＋TBI, and PACAP＋TBI. Normal saline or PACAP （1 μg/5 μL） was administered intracerebroventricularly 20 minutes before TBI. Right parietal cortical contusion was produced via a weight-dropping method. Brains were extracted 24 hours after trauma. Histological changes in brains were examined by HE staining. The numbers of CD4＋ and CD8＋ T cells in blood and the spleen were detected via flow cytometry.RESULTS： In injured brain regions, edema, hemorrhage, inflammatory cell infiltration, and swollen and degenerated neurons were observed under a light microscope, and the neurons were disorderly arrayed in the hippocampi. Compared to the sham group, average CD4＋ CD8＋ lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBl＋vehicle, and CD4- CD8＋ were increased. In rats administered PACAP prior to TBI, damage was attenuated as evidenced by significantly increased CD4＋, and decreased CD8＋, T lymphocytes in blood and the spleen.CONCLUSION： Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.展开更多
目的探讨半乳凝素-10(galectin-10)在CD4+CD25+CD127low/-调节性T细胞(regulatory T cell,Treg)中的表达及其与Foxp3的相关性。方法通过流式细胞术分选健康人外周血和胃癌患者癌旁淋巴结中Treg细胞与CD4+CD25-CD127+T细胞,经微量抽提RNA...目的探讨半乳凝素-10(galectin-10)在CD4+CD25+CD127low/-调节性T细胞(regulatory T cell,Treg)中的表达及其与Foxp3的相关性。方法通过流式细胞术分选健康人外周血和胃癌患者癌旁淋巴结中Treg细胞与CD4+CD25-CD127+T细胞,经微量抽提RNA后,用RT-PCR检测galectin-10和Foxp3的mRNA表达水平。结果galectin-10和Foxp3的mRNA显著表达于Treg细胞,几乎不表达于CD4+CD25-CD127+T细胞。健康人外周血和胃癌患者癌旁淋巴结中的结果一致。结论ga-lectin-10基因高表达于Treg细胞中,可以作为Treg细胞一项新的标志物。展开更多
文摘目的:分析CD4^(+)T淋巴细胞计数和病毒载量与艾滋病患者机会性感染的相关性,为预防和治疗艾滋病患者的机会性感染提供参考。方法:选取2023年1月—12月现住址为吉安市的1377例接受抗艾滋病治疗的艾滋病患者作为研究对象,按照是否发生机会性感染分为感染组与未感染组,采集所有艾滋病患者的年龄、性别、婚姻状况、文化程度、感染途径、临床分期等基本临床信息,以及血中CD4^(+)T淋巴细胞计数和病毒载量等指标的水平,分析机会性感染的发生与CD4^(+)T淋巴细胞计数、病毒载量的关系。结果:1377例接受抗病毒治疗的艾滋病患者中发生机会性感染的有130例(感染发生率为9.44%),感染主要累及呼吸系统(55例,占42.31%),其次为皮肤黏膜(25例,占19.23%);机会性感染患者的CD4^(+)T淋巴细胞计数水平显著低于非机会性感染患者(145.28±56.39 vs 211.25±57.10,P<0.05),而其病毒载量>104/mL的比例则明显高于非机会性感染患者(76.15%vs22.05%,P<0.05);Spearman相关分析结果显示,艾滋病患者发生机会性感染与病毒载量呈正相关(r=0.356,P<0.001),而与CD4^(+)T淋巴细胞计数呈负相关(r=-0.321,P<0.001)。结论:艾滋病患者发生机会性感染的概率较高,而CD4^(+)T淋巴细胞计数的下降和病毒载量的上升会增加机会性感染的发生风险,临床应加强对风险患者的管理和照护,以减少或及早发现机会性感染的发生。
文摘BACKGROUND： The effect of pituitary adenylate cyclase activating polypeptide （PACAP） during traumatic brain injury （TBI） and whether it can modulate secondary injury has not been reported previously. The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.METHODS： Male Sprague Dawley rats were randomly divided into 3 treatment groups （n=6, each）： sham-operated, vehicle （normal saline）＋TBI, and PACAP＋TBI. Normal saline or PACAP （1 μg/5 μL） was administered intracerebroventricularly 20 minutes before TBI. Right parietal cortical contusion was produced via a weight-dropping method. Brains were extracted 24 hours after trauma. Histological changes in brains were examined by HE staining. The numbers of CD4＋ and CD8＋ T cells in blood and the spleen were detected via flow cytometry.RESULTS： In injured brain regions, edema, hemorrhage, inflammatory cell infiltration, and swollen and degenerated neurons were observed under a light microscope, and the neurons were disorderly arrayed in the hippocampi. Compared to the sham group, average CD4＋ CD8＋ lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBl＋vehicle, and CD4- CD8＋ were increased. In rats administered PACAP prior to TBI, damage was attenuated as evidenced by significantly increased CD4＋, and decreased CD8＋, T lymphocytes in blood and the spleen.CONCLUSION： Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.
文摘目的探讨半乳凝素-10(galectin-10)在CD4+CD25+CD127low/-调节性T细胞(regulatory T cell,Treg)中的表达及其与Foxp3的相关性。方法通过流式细胞术分选健康人外周血和胃癌患者癌旁淋巴结中Treg细胞与CD4+CD25-CD127+T细胞,经微量抽提RNA后,用RT-PCR检测galectin-10和Foxp3的mRNA表达水平。结果galectin-10和Foxp3的mRNA显著表达于Treg细胞,几乎不表达于CD4+CD25-CD127+T细胞。健康人外周血和胃癌患者癌旁淋巴结中的结果一致。结论ga-lectin-10基因高表达于Treg细胞中,可以作为Treg细胞一项新的标志物。
