To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conduct...To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before harvest.The appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of storage.The results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli color.Furthermore,during the growth stage,total sugar accumulation was higher in the 5-ARG group.In addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest broccoli.In conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of broccoli.Therefore,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.展开更多
In order to study the responding of different growth state on low temperature in Broccoli (Brassica oleracea var. italica), we took prematurity broccoli hybrid as the objects. It was found that growth state was varied...In order to study the responding of different growth state on low temperature in Broccoli (Brassica oleracea var. italica), we took prematurity broccoli hybrid as the objects. It was found that growth state was varied under different sowing time , moreover, stem became wider with the increasing of light density at the same leaf age. Seedling age of responding to low temperature vernalization only when they grew five leaves above and with over 3.03 ±0.07 centimeter stalk width in 'Qingfeng Broccoli 103'. The older leaf age was and the stronger plant was, the more sensitive and the shorter demanded duration time on low temperature was, and the shorter time required when the plants entered into critical period of floral bud differentiation.展开更多
Eight F<sub>1</sub>-hybrid cultivars of broccoli were studied.We obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one cultivar.The leavesof propagated plan...Eight F<sub>1</sub>-hybrid cultivars of broccoli were studied.We obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one cultivar.The leavesof propagated plantlets in vitro were cut into 1—2mm pieces,isolated with an enzyme solutioncontaining 2% cellulase and 1%macerase on a rotary shaker（50 rpm,21℃,3h,2500 lux light）,and purified with a 0.5M sucrose solution.The purified protoplasts were placed on a drop of 1%agarose.2—3 ml liquid medium was added around the agarose drops,and all of the cultures wereincubated at 25℃ under light（4000 lux）for 16 hours.3—5 days after isolation the cell divisionwas found.About 7 days after incubation 4 multicellular colonies were formed.After 3—5 wksome p-calli were developed.When the p-calli were 2—3 mm in diameter it was transferred to asolidified medium.Once they were developed to 1 cm in diameter they were transferred on a re-generation medium.About 5 months after incubation some roots and shoots grown from the calliwere展开更多
The screening for Black rot(Xanthomonas campestris PV.campestris)resistance in broccoli (Brassica oleracea L.Var.italica Planch) lines plays an important role in breeding for Black rot resistance and is of great value...The screening for Black rot(Xanthomonas campestris PV.campestris)resistance in broccoli (Brassica oleracea L.Var.italica Planch) lines plays an important role in breeding for Black rot resistance and is of great value to practical utilization. The broccoli in bred line"91-9318-4"was used as the experimental material in the trial.X 0-2 strain of Xanthomonas campestris PV.campestris was and purified from the infected broccoli plants in the field.The spraying method was adopted in the inoculation.The trial was designed as the randomized blocks of two factors with three replications.Factor A was the foliar age and factor B was the concetrations of inoculum.Factor A had three levels A 1,A 2 and A 3 (the 2nd 4th and 6th true leaf stage),factor B had three levels B 1,B 2 and B 3(10 6 counts/mL,10 7 counts/mL and 10 8 counts/mL). Results of this experiment showed that the best method for screening Black rot resistance in broccoli is ①foliar age:the 4th true leaf stage;②concetration of inoculums;10 7 counts/mL;③temperature at 20～30℃ (from the end of June to the end of July at he local climate condition.) 26 breeding materials of broccoli were identified for resistance to Black rot by means of the acquired method.One resistant breeding material was obtained,the identifying results showed that this screening method is practical.展开更多
该研究采用远红外干燥、热风干燥和真空干燥对西兰花进行干燥处理,研究不同温度(60、65、70、75、80℃)对西兰花干燥特性和理化性质的影响。结果表明,与真空干燥相比,远红外干燥和热风干燥下将西兰花干燥至终点时所需时间更短、干燥速...该研究采用远红外干燥、热风干燥和真空干燥对西兰花进行干燥处理,研究不同温度(60、65、70、75、80℃)对西兰花干燥特性和理化性质的影响。结果表明,与真空干燥相比,远红外干燥和热风干燥下将西兰花干燥至终点时所需时间更短、干燥速率更快;干燥西兰花是一个从介质吸收能量实现脱水的非自发过程,且温度越高,有效水分扩散系数越大,活化能越低,越易干燥;8种数学模型拟合结果表明,Wang and Singh经验模型是描述西兰花干燥的最佳数学模型;干燥条件对西兰花色泽有显著影响,真空干燥时色泽保护效果更好;65℃热风干燥复水比(8.82±0.11)、膨胀力[(10.33±0.34)mL/g]最高;80℃热风干燥西兰花复水比(6.98±0.2)、膨胀力[(7.77±0.12)mL/g]最低。该研究为西兰花的加工、贮藏提供了参考价值。展开更多
文摘To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before harvest.The appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of storage.The results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli color.Furthermore,during the growth stage,total sugar accumulation was higher in the 5-ARG group.In addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest broccoli.In conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of broccoli.Therefore,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.
文摘In order to study the responding of different growth state on low temperature in Broccoli (Brassica oleracea var. italica), we took prematurity broccoli hybrid as the objects. It was found that growth state was varied under different sowing time , moreover, stem became wider with the increasing of light density at the same leaf age. Seedling age of responding to low temperature vernalization only when they grew five leaves above and with over 3.03 ±0.07 centimeter stalk width in 'Qingfeng Broccoli 103'. The older leaf age was and the stronger plant was, the more sensitive and the shorter demanded duration time on low temperature was, and the shorter time required when the plants entered into critical period of floral bud differentiation.
文摘Eight F<sub>1</sub>-hybrid cultivars of broccoli were studied.We obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one cultivar.The leavesof propagated plantlets in vitro were cut into 1—2mm pieces,isolated with an enzyme solutioncontaining 2% cellulase and 1%macerase on a rotary shaker（50 rpm,21℃,3h,2500 lux light）,and purified with a 0.5M sucrose solution.The purified protoplasts were placed on a drop of 1%agarose.2—3 ml liquid medium was added around the agarose drops,and all of the cultures wereincubated at 25℃ under light（4000 lux）for 16 hours.3—5 days after isolation the cell divisionwas found.About 7 days after incubation 4 multicellular colonies were formed.After 3—5 wksome p-calli were developed.When the p-calli were 2—3 mm in diameter it was transferred to asolidified medium.Once they were developed to 1 cm in diameter they were transferred on a re-generation medium.About 5 months after incubation some roots and shoots grown from the calliwere
文摘The screening for Black rot(Xanthomonas campestris PV.campestris)resistance in broccoli (Brassica oleracea L.Var.italica Planch) lines plays an important role in breeding for Black rot resistance and is of great value to practical utilization. The broccoli in bred line"91-9318-4"was used as the experimental material in the trial.X 0-2 strain of Xanthomonas campestris PV.campestris was and purified from the infected broccoli plants in the field.The spraying method was adopted in the inoculation.The trial was designed as the randomized blocks of two factors with three replications.Factor A was the foliar age and factor B was the concetrations of inoculum.Factor A had three levels A 1,A 2 and A 3 (the 2nd 4th and 6th true leaf stage),factor B had three levels B 1,B 2 and B 3(10 6 counts/mL,10 7 counts/mL and 10 8 counts/mL). Results of this experiment showed that the best method for screening Black rot resistance in broccoli is ①foliar age:the 4th true leaf stage;②concetration of inoculums;10 7 counts/mL;③temperature at 20～30℃ (from the end of June to the end of July at he local climate condition.) 26 breeding materials of broccoli were identified for resistance to Black rot by means of the acquired method.One resistant breeding material was obtained,the identifying results showed that this screening method is practical.
文摘该研究采用远红外干燥、热风干燥和真空干燥对西兰花进行干燥处理,研究不同温度(60、65、70、75、80℃)对西兰花干燥特性和理化性质的影响。结果表明,与真空干燥相比,远红外干燥和热风干燥下将西兰花干燥至终点时所需时间更短、干燥速率更快;干燥西兰花是一个从介质吸收能量实现脱水的非自发过程,且温度越高,有效水分扩散系数越大,活化能越低,越易干燥;8种数学模型拟合结果表明,Wang and Singh经验模型是描述西兰花干燥的最佳数学模型;干燥条件对西兰花色泽有显著影响,真空干燥时色泽保护效果更好;65℃热风干燥复水比(8.82±0.11)、膨胀力[(10.33±0.34)mL/g]最高;80℃热风干燥西兰花复水比(6.98±0.2)、膨胀力[(7.77±0.12)mL/g]最低。该研究为西兰花的加工、贮藏提供了参考价值。
