A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complet...A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.展开更多
vegetation restoration is a main ecological remediation technology for greening saline and alkaline soils.The objectives of this study were to determine the effect of1-aminobenzotriazole(ABT-1) on the growth and physi...vegetation restoration is a main ecological remediation technology for greening saline and alkaline soils.The objectives of this study were to determine the effect of1-aminobenzotriazole(ABT-1) on the growth and physiology of Tamarix chinensis under salt stress and to determine a suitable ABT-1 concentration and soil salinity(Sc) for propagating T.chihehsis-cuttings.Cuttings were soaked in water and ABT-1 solutions at three concentrations(50,100,and 200 mg L^(-1)) and propagated in pots containing four soil salinity levels,mild(0.3%),moderate(0.6%),and severe(0.9% and 1.2%),and compared with a control.The cuttings were measured to determine growth indices and physiological and biochemical indices(e.g.,chlorophyll content,superoxide dismutase activity,peroxidase activity,and malondialdehyde content).ABT-1 was effective in improving survival,growth,and physiological processes of cuttings under salt stress.However,there was a threshold effect when using ABT-1 to facilitate propagation under salt stress.ABT-1 effects were insignificant when applied at low concentrations(<100 mg L^(-1)).At a high concentration(> 100 mg L^(-1)),ABT-1 limited growth and physiological activities.Under a salt stress level(Sc ≤0.9%),ABT applied at a 100 mg L^(-1)concentration increased chlorophyll content and superoxide dismutase and peroxidase activities in the leaves and reduced malondialdehyde accumulation and membrane lipid peroxidation effects.As a result,ABT-1 enhanced the resistance of T.chinensis to salt stress.However,under high salt stress(>0.9%) and ABT-1 concentration(> 100 mg L^(-1)),the physiological regulatory ability of T.chinensis seedlings weakened.T.chinensis grew well at a salt stress ≤0.9% and ABT ≤100 mg L^(-1) and exhibited relatively high physiological regulatory ability and high salt adaptability.展开更多
基金supported financially by the project‘Research and Development on Technology and Key Equipment for New Type of Industrialized and Healthy Animal Husbandry'funded by the Chinese Ministry of Science and Technology(2006BAD 14B02-6)11th Five Years Key Programs for Science and Technology Development of China(2006BAD01A08-07)+1 种基金Hubei Province Key Project of Science and Technology (2006AA201B24)Wuhan City Key Project of Industrialization(200720112026)
文摘A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.
基金supported financially by the National Natural Science Foundation of China (No.31770761)the Forestry Science and Technology Innovation Project of Shandong province (2019LY006)+1 种基金the Natural Science Foundation of Shandong province (No.ZR2017LEE023)the Taishan Scholars Program of Shandong province,P.R.China(No.TSQN201909152)。
文摘vegetation restoration is a main ecological remediation technology for greening saline and alkaline soils.The objectives of this study were to determine the effect of1-aminobenzotriazole(ABT-1) on the growth and physiology of Tamarix chinensis under salt stress and to determine a suitable ABT-1 concentration and soil salinity(Sc) for propagating T.chihehsis-cuttings.Cuttings were soaked in water and ABT-1 solutions at three concentrations(50,100,and 200 mg L^(-1)) and propagated in pots containing four soil salinity levels,mild(0.3%),moderate(0.6%),and severe(0.9% and 1.2%),and compared with a control.The cuttings were measured to determine growth indices and physiological and biochemical indices(e.g.,chlorophyll content,superoxide dismutase activity,peroxidase activity,and malondialdehyde content).ABT-1 was effective in improving survival,growth,and physiological processes of cuttings under salt stress.However,there was a threshold effect when using ABT-1 to facilitate propagation under salt stress.ABT-1 effects were insignificant when applied at low concentrations(<100 mg L^(-1)).At a high concentration(> 100 mg L^(-1)),ABT-1 limited growth and physiological activities.Under a salt stress level(Sc ≤0.9%),ABT applied at a 100 mg L^(-1)concentration increased chlorophyll content and superoxide dismutase and peroxidase activities in the leaves and reduced malondialdehyde accumulation and membrane lipid peroxidation effects.As a result,ABT-1 enhanced the resistance of T.chinensis to salt stress.However,under high salt stress(>0.9%) and ABT-1 concentration(> 100 mg L^(-1)),the physiological regulatory ability of T.chinensis seedlings weakened.T.chinensis grew well at a salt stress ≤0.9% and ABT ≤100 mg L^(-1) and exhibited relatively high physiological regulatory ability and high salt adaptability.
