欧洲白桦各无性系试管苗诱导生根的能力从易到难依次为:2/86、1/86、4N、G 1;以1/2M S为基本培养基。对于无性系4N,单独使用NAA(0.3 m g.L-1)比单独使用IBA和同时使用NAA与IBA更有利于试管苗不定根诱导;0.1 m g.L-1NAA与0.1 m g.L-1IBA...欧洲白桦各无性系试管苗诱导生根的能力从易到难依次为:2/86、1/86、4N、G 1;以1/2M S为基本培养基。对于无性系4N,单独使用NAA(0.3 m g.L-1)比单独使用IBA和同时使用NAA与IBA更有利于试管苗不定根诱导;0.1 m g.L-1NAA与0.1 m g.L-1IBA配合使用,诱导无性系1/86、2/86的试管苗生根,效果最佳。白桦试管苗木质化程度越高,其生根率有下降的趋势。试管苗移栽采用腐殖土∶细沙∶园土(1∶1∶1)或腐殖土∶园土(2∶1)作栽培基质效果较好。试管苗在生根培养基上培养的天数以及生根试管苗的生长状况对移栽成活率影响明显,试管苗不定根伸长最快的时期,移栽成活率最高。展开更多
Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population...Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population comprising of 79 progenies from the cross between Betula pendula and B.platyphylla was obtained.A set of 1 000 random oligonucleotide primers were screened,and 178 primers were selected to generate RAPD markers within a sample of 79 F\-1 progenies.A total of 296 segregating sites was identified.Among them,273 sites belonged to 1∶1 segregating site,and 36 sites were found distorted from the 1∶1 ratio.The 273 sites segregating 1∶1(testcross configuration) were used to construct parent-specific linkage group,among which 137 sites were found for B.pendula and 136 sites for B.platyphylla.The resulting linkage maps consisted of 117 marker sites in 12 groups(four or more sites per group),2 triples and 3 pairs for B.pendula,which covered the map distance about 1 533.3 cM(Kosambi units),and the average map distance between adjacent markers was 16.4 cM.In the B.platyphylla,162 linked marker sites were integrated into 12 groups(four or more sites per group),2 triples and 4 pairs,which covered the map distance about 1 847.8 cM,and the average map distance between adjacent markers was 19.2 cM.The calculated genome coverage rate of B.pendula and B.platyphylla were respectively 68.5% and 68.9%.Further study is required to integrate the two maps to one density map and to locate important genes on the maps.展开更多
文摘欧洲白桦各无性系试管苗诱导生根的能力从易到难依次为:2/86、1/86、4N、G 1;以1/2M S为基本培养基。对于无性系4N,单独使用NAA(0.3 m g.L-1)比单独使用IBA和同时使用NAA与IBA更有利于试管苗不定根诱导;0.1 m g.L-1NAA与0.1 m g.L-1IBA配合使用,诱导无性系1/86、2/86的试管苗生根,效果最佳。白桦试管苗木质化程度越高,其生根率有下降的趋势。试管苗移栽采用腐殖土∶细沙∶园土(1∶1∶1)或腐殖土∶园土(2∶1)作栽培基质效果较好。试管苗在生根培养基上培养的天数以及生根试管苗的生长状况对移栽成活率影响明显,试管苗不定根伸长最快的时期,移栽成活率最高。
文摘Based on the genetic inheritance and segregation of random amplified polymorphism DNA(RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo-testcross strategy.A segregating population comprising of 79 progenies from the cross between Betula pendula and B.platyphylla was obtained.A set of 1 000 random oligonucleotide primers were screened,and 178 primers were selected to generate RAPD markers within a sample of 79 F\-1 progenies.A total of 296 segregating sites was identified.Among them,273 sites belonged to 1∶1 segregating site,and 36 sites were found distorted from the 1∶1 ratio.The 273 sites segregating 1∶1(testcross configuration) were used to construct parent-specific linkage group,among which 137 sites were found for B.pendula and 136 sites for B.platyphylla.The resulting linkage maps consisted of 117 marker sites in 12 groups(four or more sites per group),2 triples and 3 pairs for B.pendula,which covered the map distance about 1 533.3 cM(Kosambi units),and the average map distance between adjacent markers was 16.4 cM.In the B.platyphylla,162 linked marker sites were integrated into 12 groups(four or more sites per group),2 triples and 4 pairs,which covered the map distance about 1 847.8 cM,and the average map distance between adjacent markers was 19.2 cM.The calculated genome coverage rate of B.pendula and B.platyphylla were respectively 68.5% and 68.9%.Further study is required to integrate the two maps to one density map and to locate important genes on the maps.
