目的建立并评价基于光激化学发光分析(LICA)平台的牛奶过敏单组分(Bos d 5)特异性IgG_4抗体(sIgG_4)定量检测方法(sIgG_4-LICA)。方法以生物素标记的Bos d 5抗原、待检血清、抗人IgG_4抗体包被的发光微球和链霉亲合素包被的感光微球组...目的建立并评价基于光激化学发光分析(LICA)平台的牛奶过敏单组分(Bos d 5)特异性IgG_4抗体(sIgG_4)定量检测方法(sIgG_4-LICA)。方法以生物素标记的Bos d 5抗原、待检血清、抗人IgG_4抗体包被的发光微球和链霉亲合素包被的感光微球组成分析体系,基于LICA平台,采用间接法模式,建立Bos d 5 sIgG_4抗体定量检测方法(sIgG_4-LICA),并对其进行条件优化和性能评价。结果 sIgG_4-LICA方法的批内精密度、日间精密度和批间精密度分别为1.78%～3.13%、6.65%～8.41%和7.94%～12.30%,功能灵敏度为4.71 ng/mL,线性范围为28.13～1 800 ng/mL,线性回归方程为Y=0.98X-1.31(r^2=0.997),最大稀释度为1∶64,血红蛋白、三酰甘油、总胆红素、抗坏血酸及生物素的干扰率为-6.38%～8.60%。结论本研究建立的Bos d 5 sIgG_4抗体定量检测方法性能良好,可满足临床监测sIgG_4抗体的需要。展开更多
The Counter-Propagation Deduction (CPD) method suggested by the authors is a new one for analyzing electromagnetic (EM) field in stratified media. The mechanism or the method is briefly introduced, and the rield in a ...The Counter-Propagation Deduction (CPD) method suggested by the authors is a new one for analyzing electromagnetic (EM) field in stratified media. The mechanism or the method is briefly introduced, and the rield in a layered medium in the presence or slant incidence of a perpendicularly or parallelly polarized electric wave is investigated. Some illustrative examples show that the method is capable of providing the direct mathematic expressions of the fields in an arbitrary layer, and is convenient for numerical evaluation through an unified program, irrespective of the number of medium layers.展开更多
Background:Trees and forests in drylands help mitigate the challenges through provision of economic products and vital environmental services such as habitat for biodiversity,prevention of erosion and desertification,...Background:Trees and forests in drylands help mitigate the challenges through provision of economic products and vital environmental services such as habitat for biodiversity,prevention of erosion and desertification,regulation of water,microclimate,and soil fertility.The condition and changes in dry forests can be assessed by using ecological indicators able to quantify spatial and temporal changes in vegetation.One of the ways to determine the condition of the forest is to study the dominant tree species and its regeneration.Our study aimed to assess whether the abundance of Prosopis flexuosa saplings is affected by environmental and biological factors.Results:To evaluate the first variables we used data from remote sensing such as satellite images and Aster Global Digital Model(GDEM).The second set of variables was about exotic and native ungulates and we used feces of these animals and camera traps to take data.We found that sapling abundance related positively to sandy substrates and negatively to Wetness Index.On the other hand,in relation to biological variables,the abundance of saplings was positively affected by density of adult trees and by number of seeds dispersed by equines,but space use by Lama guanicoe had a negative relationship with saplings.This research shows that P.flexuosa saplings are benefited from sandy substrates and the conditions around adult trees.In addition to this,we found that exotic ungulates in low densities have neutral(i.e.cattle)or positive(i.e.equines)effects on sapling abundance.Conclusions:Based on these findings,we conclude that regeneration of the population of P.flexuosa in our study area has no major problems.In addition,we corroborated that the presence of exotic and domestic ungulates in low densities does not have deleterious consequences for saplings of the dominant tree,P.flexuosa.展开更多
Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qi...Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qinghai Province. This paper was studied for the morphological characteristics of the skin and hair coat of yak and their adaptability to the cold living environment in Qinghai-Tibetan plateau. The results indicated that it should be of vital importance marker for the morphological characteristics of the skin and hair coat with two type of yaks at 3 and 12 age in months and their regulations of growth and development to adapt to the cold living environment in Qinghai-Tibetan plateau,The main morphological indexes of the skin and hair coat of the rearing new yak strain were higher than those of the domestic yaks at the same age in months, or speak precisely, the new yak strain was more powerful adaptable to the alpine cold living environment than that of the domestic yaks. The resvlts above provided scientific basis for the native strain breeding of yak and the new yak strain rearing.展开更多
文摘目的建立并评价基于光激化学发光分析(LICA)平台的牛奶过敏单组分(Bos d 5)特异性IgG_4抗体(sIgG_4)定量检测方法(sIgG_4-LICA)。方法以生物素标记的Bos d 5抗原、待检血清、抗人IgG_4抗体包被的发光微球和链霉亲合素包被的感光微球组成分析体系,基于LICA平台,采用间接法模式,建立Bos d 5 sIgG_4抗体定量检测方法(sIgG_4-LICA),并对其进行条件优化和性能评价。结果 sIgG_4-LICA方法的批内精密度、日间精密度和批间精密度分别为1.78%～3.13%、6.65%～8.41%和7.94%～12.30%,功能灵敏度为4.71 ng/mL,线性范围为28.13～1 800 ng/mL,线性回归方程为Y=0.98X-1.31(r^2=0.997),最大稀释度为1∶64,血红蛋白、三酰甘油、总胆红素、抗坏血酸及生物素的干扰率为-6.38%～8.60%。结论本研究建立的Bos d 5 sIgG_4抗体定量检测方法性能良好,可满足临床监测sIgG_4抗体的需要。
文摘The Counter-Propagation Deduction (CPD) method suggested by the authors is a new one for analyzing electromagnetic (EM) field in stratified media. The mechanism or the method is briefly introduced, and the rield in a layered medium in the presence or slant incidence of a perpendicularly or parallelly polarized electric wave is investigated. Some illustrative examples show that the method is capable of providing the direct mathematic expressions of the fields in an arbitrary layer, and is convenient for numerical evaluation through an unified program, irrespective of the number of medium layers.
基金supported by“The chica,the retamo,and the algarrobo:umbrella species for the conservation of the Native Forest of the Ischigualasto Provincial Park and nearby zones.Biological interactions,effects of human activities and their mitigation”,Plan for the Conservation of Native Forests Law 26.331.
文摘Background:Trees and forests in drylands help mitigate the challenges through provision of economic products and vital environmental services such as habitat for biodiversity,prevention of erosion and desertification,regulation of water,microclimate,and soil fertility.The condition and changes in dry forests can be assessed by using ecological indicators able to quantify spatial and temporal changes in vegetation.One of the ways to determine the condition of the forest is to study the dominant tree species and its regeneration.Our study aimed to assess whether the abundance of Prosopis flexuosa saplings is affected by environmental and biological factors.Results:To evaluate the first variables we used data from remote sensing such as satellite images and Aster Global Digital Model(GDEM).The second set of variables was about exotic and native ungulates and we used feces of these animals and camera traps to take data.We found that sapling abundance related positively to sandy substrates and negatively to Wetness Index.On the other hand,in relation to biological variables,the abundance of saplings was positively affected by density of adult trees and by number of seeds dispersed by equines,but space use by Lama guanicoe had a negative relationship with saplings.This research shows that P.flexuosa saplings are benefited from sandy substrates and the conditions around adult trees.In addition to this,we found that exotic ungulates in low densities have neutral(i.e.cattle)or positive(i.e.equines)effects on sapling abundance.Conclusions:Based on these findings,we conclude that regeneration of the population of P.flexuosa in our study area has no major problems.In addition,we corroborated that the presence of exotic and domestic ungulates in low densities does not have deleterious consequences for saplings of the dominant tree,P.flexuosa.
文摘Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qinghai Province. This paper was studied for the morphological characteristics of the skin and hair coat of yak and their adaptability to the cold living environment in Qinghai-Tibetan plateau. The results indicated that it should be of vital importance marker for the morphological characteristics of the skin and hair coat with two type of yaks at 3 and 12 age in months and their regulations of growth and development to adapt to the cold living environment in Qinghai-Tibetan plateau,The main morphological indexes of the skin and hair coat of the rearing new yak strain were higher than those of the domestic yaks at the same age in months, or speak precisely, the new yak strain was more powerful adaptable to the alpine cold living environment than that of the domestic yaks. The resvlts above provided scientific basis for the native strain breeding of yak and the new yak strain rearing.
