Xylo-oligosaccharides(XOSs)are a category of functional oligosaccharides primarily composed of 2-7 xylose units linked byβ-1,4 glycosidic bonds.They are recognized as soluble dietary fibers with prebiotic properties....Xylo-oligosaccharides(XOSs)are a category of functional oligosaccharides primarily composed of 2-7 xylose units linked byβ-1,4 glycosidic bonds.They are recognized as soluble dietary fibers with prebiotic properties.Recently, there has been significant interest in manufacturing XOSs from xylan extracted from lignocellulosic biomass using enzyme catalysis under mild conditions. In this work, the arabinofuranosidase Abf62A gene was cloned from Aspergillus usamii genomic DNA through sequential molecular processes and expressed in Pichia pastoris X33. The xylan (100 g/L) extracted xylan in wheat straw (WS) was biologically hydrolyzed into 50.32 g/L of XOSs by xylanase Xyn11A (300 U/g substrate) and arabinofuranase Abf62A (20 U/g substrate), which indicated a notable synergistic effect compared to the 34.42 g/L XOSs produced via Xyn11A. The 50.32 g/L of XOSs products comprised xylobiose (31.71 g/L), xylotriose (15.92 g/L), xylotetraose (1.65 g/L) and xylopentaose (1.04 g/L). Notably, the combined content of xylobiose and xylotriose accounted for up to 94.7%. The XOSs purified from the enzyme hydrolysate could effectually scavenge free radicals, and the antioxidant activity was more than 90%. In summary, XOSs were biologically manufactured from wheat straw xylan through the synergistic biocatalysis via xylanase and arabinofuranosidase Abf62A in a green and sustainable way, rending one kind of prebiotic oligosaccharides with substantial positive effects on human and animal health.展开更多
Soybean seed oil has always been the primary focus of researches on improving the quality of soybean.Triacylglycerol,which is composed of fatty acids and glycerol,serves as the main storage form of soybean seed oil.Th...Soybean seed oil has always been the primary focus of researches on improving the quality of soybean.Triacylglycerol,which is composed of fatty acids and glycerol,serves as the main storage form of soybean seed oil.Therefore,the biosynthesis of fatty acids and the assembly of triacylglycerol significantly affect the quality of soybean seed oil.The process of soybean seed oil synthesis was comprehensively analyzed by examining the synthesis and activation of acetyl-CoA,fatty acid biosynthesis and desaturation,triacylglycerol assembly,and other related processes,additionally,this analysis identified the key enzymes and transcription factors involved in each link of the oil synthesis process.It was found that the researches on the key enzymes of carbon source regulation,pyruvate dehydrogenase complex and acetyl-CoA carboxylase,in the genetic network of soybean seed oil synthesis,were somewhat limited.Analyzing their structures and functions would aid in further understanding the molecular mechanism of soybean fatty acid synthesis,which would be beneficial for enhancing soybean oil content and synthesis efficiency.Based on summarizing the results of genetic analysis of soybean oil and fatty acids,the prospects for mechanism analysis and advancements in biological breeding techniques for improving soybean oil quality at the molecular level were discussed.展开更多
Background:Mature cotton fiber secondary cell wall comprises largely of cellulose(>90%)and small amounts of xylan and lignin.Little is known about the cotton fiber xylan biosynthesis by far.Results:To comprehensive...Background:Mature cotton fiber secondary cell wall comprises largely of cellulose(>90%)and small amounts of xylan and lignin.Little is known about the cotton fiber xylan biosynthesis by far.Results:To comprehensively survey xylan biosynthetic genes in cotton fiber,we identified five IRX9,five IRX10,one IRX14,six IRX15,two FRA8,one PARVUS,eight GUX,four GXM,two RWA,two AXY9,13 TBL genes by using phylogenetic analysis coupled with expression profile analysis and co-expression analyses.In addition,we also identified two GT61 members,two GT47 members,and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized.These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis.Further complementation analysis indicated that one IRX10 like and two FRA8 related genes were able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in their respective Arabidopsis mutant.We conclude that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis.Conclusion:The list of 55 cotton genes presented here provides not only a solid basis to uncover the biosynthesis of xylan in cotton fiber,but also a genetic resource potentially useful for future studies aiming at fiber improvement via biotechnological approaches.展开更多
Cotton(Gossypium hirsutum L.) is the leading fiber crop and one of the mainstays of the economy in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear
A distinct set of homoeologous cellulose synthase catalytic subunit(CesA) genes are coordinately up-regulated with the onset of secondary wall formation in cotton fiber as shown by quantitative-RT-
Cotton has enormous economic potential,providing high-quality protein,oil,and fibre.But the comprehensive utilization of cottonseed is limited by the presence of pigment gland and its inclusion.Pigment gland is a comm...Cotton has enormous economic potential,providing high-quality protein,oil,and fibre.But the comprehensive utilization of cottonseed is limited by the presence of pigment gland and its inclusion.Pigment gland is a common characteristic of Gossypium genus and its relatives,appearing as visible dark opaque dots in most tissues and organs of cotton plants.Secondary metabolites,such as gossypol,synthesized and stored in the cavities of pigment glands act as natural phytoalexins,but are toxic to humans and other monogastric animals.However,only a few cotton genes have been identified as being associated with pigment gland morphogenesis to date,and the developmental processes and regulatory mechanism involved in pigment gland formation remain largely unclear.Here,the research progress on the process of pigment gland morphogenesis and the genetic basis of cotton pigment glands is reviewed,for providing a theoretical basis for cultivating cotton with the ideal pigment gland trait.展开更多
Cellulose synthesis could play an important rolein determining certain aspects of cotton fiberquality,and despite it’s abundance in nature,little is known about the biosynthesis of thispolymer.Recent advances in unde...Cellulose synthesis could play an important rolein determining certain aspects of cotton fiberquality,and despite it’s abundance in nature,little is known about the biosynthesis of thispolymer.Recent advances in understanding thesynthesis of cellulose come from the analysis ofArabidopsis thaliana cellulose-deficient mutants,and the isolation of a number of genes展开更多
Background Cotton is extremely affected by severe natural stresses.Drought is one of the most serious abiotic stress that adversely influences cotton growth,productivity,and fiber quality.Previous studies indicate tha...Background Cotton is extremely affected by severe natural stresses.Drought is one of the most serious abiotic stress that adversely influences cotton growth,productivity,and fiber quality.Previous studies indicate that basic leucinezipper(bZIP)transcription factors are involved in the response of plants to various stresses.However,the molecular function and regulatory mechanism of GhVIP1 in response to drought stress are still unknown.Results In this research,GhVIP1 was cloned from a drought-tolerant variety.Expression of GhVIP1 was up-regulated in response to multiple abiotic stresses,especially under drought stress.And GhVIP1 was highly expressed in the root,stem,and 10 days post-anthesis ovule.Inhibiting the expression of GhVIP1 in cotton using the virus-induced gene silencing method resulted in higher electrical conductivity in leaves,but lower water content under drought stress compared with the WT plant.Overexpression of GhVIP1 in Arabidopsis enhanced plant drought tolerance through increasing the seed germination rate and improving the development of root.The exogenous expression of GhVIP1 up-regulated the transcription of genes associated with drought response and proline biosynthesis during drought stress in Arabidopsis.Conclusion In summary,these results indicated that GhVIP1 played a positive role in plants’response to drought stress.The use of GhVIP1 via modern biotechnology might facilitate the improvement of drought tolerance in cotton cultivars.展开更多
The developing cotton fiber is considered as amodel system for studying the function ofmicrotubules in plant cell elongation and cellwall biosynthesis.It has been shown thatmicrotubules undergo active dynamic changesd...The developing cotton fiber is considered as amodel system for studying the function ofmicrotubules in plant cell elongation and cellwall biosynthesis.It has been shown thatmicrotubules undergo active dynamic changesduring fiber development.At the molecularlevel,Dixon et al demonstrated that two alpha-tubulin and two beta-tubulin展开更多
Cotton fibers are single-cell trichomes derivedfrom the epidermis of cotton ovules.Analysis oftranscripts in developing fiber cells may providevaluable information to understand mechanismsregulation fiber initiation a...Cotton fibers are single-cell trichomes derivedfrom the epidermis of cotton ovules.Analysis oftranscripts in developing fiber cells may providevaluable information to understand mechanismsregulation fiber initiation and growth,as well asto cotton breeding.We have made an array ofmore than 5000 clones isolated from a cottonovule(and the fiber)cDNA library.展开更多
所属学科：生物物理学、生物化学及分子生物学,细胞生物学,生物技术与生物工程开始日期：2017-03-22结束日期：2017-03-24所在国家：英国所在城市：英国具体地点：Warwick,U.K.主办单位：Royal Society of Chemistry联系电话：＋44（0）...所属学科：生物物理学、生物化学及分子生物学,细胞生物学,生物技术与生物工程开始日期：2017-03-22结束日期：2017-03-24所在国家：英国所在城市：英国具体地点：Warwick,U.K.主办单位：Royal Society of Chemistry联系电话：＋44（0）1223 434048会议网站：http：//www.rsc.展开更多
1 Du M F,Liu X G,Liu X M,Yin X M,Han S Y,Song Q S,An S H.Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori.Sci Rep,2015,DOI：10.1038/srep08110题目甘油-3-磷...1 Du M F,Liu X G,Liu X M,Yin X M,Han S Y,Song Q S,An S H.Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori.Sci Rep,2015,DOI：10.1038/srep08110题目甘油-3-磷酸酰基转移酶参与家蚕性信息素的生物合成过程摘要昆虫雌蛾通过释放性信息素吸引雄蛾与之交配。展开更多
文摘Xylo-oligosaccharides(XOSs)are a category of functional oligosaccharides primarily composed of 2-7 xylose units linked byβ-1,4 glycosidic bonds.They are recognized as soluble dietary fibers with prebiotic properties.Recently, there has been significant interest in manufacturing XOSs from xylan extracted from lignocellulosic biomass using enzyme catalysis under mild conditions. In this work, the arabinofuranosidase Abf62A gene was cloned from Aspergillus usamii genomic DNA through sequential molecular processes and expressed in Pichia pastoris X33. The xylan (100 g/L) extracted xylan in wheat straw (WS) was biologically hydrolyzed into 50.32 g/L of XOSs by xylanase Xyn11A (300 U/g substrate) and arabinofuranase Abf62A (20 U/g substrate), which indicated a notable synergistic effect compared to the 34.42 g/L XOSs produced via Xyn11A. The 50.32 g/L of XOSs products comprised xylobiose (31.71 g/L), xylotriose (15.92 g/L), xylotetraose (1.65 g/L) and xylopentaose (1.04 g/L). Notably, the combined content of xylobiose and xylotriose accounted for up to 94.7%. The XOSs purified from the enzyme hydrolysate could effectually scavenge free radicals, and the antioxidant activity was more than 90%. In summary, XOSs were biologically manufactured from wheat straw xylan through the synergistic biocatalysis via xylanase and arabinofuranosidase Abf62A in a green and sustainable way, rending one kind of prebiotic oligosaccharides with substantial positive effects on human and animal health.
基金Supported by the Heilongjiang Natural Science Foundation Joint Guidance Project(LH2021C028)。
文摘Soybean seed oil has always been the primary focus of researches on improving the quality of soybean.Triacylglycerol,which is composed of fatty acids and glycerol,serves as the main storage form of soybean seed oil.Therefore,the biosynthesis of fatty acids and the assembly of triacylglycerol significantly affect the quality of soybean seed oil.The process of soybean seed oil synthesis was comprehensively analyzed by examining the synthesis and activation of acetyl-CoA,fatty acid biosynthesis and desaturation,triacylglycerol assembly,and other related processes,additionally,this analysis identified the key enzymes and transcription factors involved in each link of the oil synthesis process.It was found that the researches on the key enzymes of carbon source regulation,pyruvate dehydrogenase complex and acetyl-CoA carboxylase,in the genetic network of soybean seed oil synthesis,were somewhat limited.Analyzing their structures and functions would aid in further understanding the molecular mechanism of soybean fatty acid synthesis,which would be beneficial for enhancing soybean oil content and synthesis efficiency.Based on summarizing the results of genetic analysis of soybean oil and fatty acids,the prospects for mechanism analysis and advancements in biological breeding techniques for improving soybean oil quality at the molecular level were discussed.
基金National Natural Science Foundation of China,China[grant numbers:3167173531970516]+1 种基金Hubei provincial Natural Science Foundation,China[grant number:2016CFA071]self-determined research funds of Central China Normal University from the colleges’basic research and operation of Ministry of Education,China(CCNU18TS021).
文摘Background:Mature cotton fiber secondary cell wall comprises largely of cellulose(>90%)and small amounts of xylan and lignin.Little is known about the cotton fiber xylan biosynthesis by far.Results:To comprehensively survey xylan biosynthetic genes in cotton fiber,we identified five IRX9,five IRX10,one IRX14,six IRX15,two FRA8,one PARVUS,eight GUX,four GXM,two RWA,two AXY9,13 TBL genes by using phylogenetic analysis coupled with expression profile analysis and co-expression analyses.In addition,we also identified two GT61 members,two GT47 members,and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized.These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis.Further complementation analysis indicated that one IRX10 like and two FRA8 related genes were able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in their respective Arabidopsis mutant.We conclude that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis.Conclusion:The list of 55 cotton genes presented here provides not only a solid basis to uncover the biosynthesis of xylan in cotton fiber,but also a genetic resource potentially useful for future studies aiming at fiber improvement via biotechnological approaches.
基金This work was supported by the National Natural Science Foundation of China (No 30370904and No 30671258)the National High Technology Research and Development Program(863 project)of China (No 2006AA10Z121)the Program for New Century Excellent Talents in University(No NCET-07-0712)
文摘Cotton(Gossypium hirsutum L.) is the leading fiber crop and one of the mainstays of the economy in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear
文摘A distinct set of homoeologous cellulose synthase catalytic subunit(CesA) genes are coordinately up-regulated with the onset of secondary wall formation in cotton fiber as shown by quantitative-RT-
基金National Key Technology R&D Program of China(2022YFF1001403)National Science Foundation of China(32101764).
文摘Cotton has enormous economic potential,providing high-quality protein,oil,and fibre.But the comprehensive utilization of cottonseed is limited by the presence of pigment gland and its inclusion.Pigment gland is a common characteristic of Gossypium genus and its relatives,appearing as visible dark opaque dots in most tissues and organs of cotton plants.Secondary metabolites,such as gossypol,synthesized and stored in the cavities of pigment glands act as natural phytoalexins,but are toxic to humans and other monogastric animals.However,only a few cotton genes have been identified as being associated with pigment gland morphogenesis to date,and the developmental processes and regulatory mechanism involved in pigment gland formation remain largely unclear.Here,the research progress on the process of pigment gland morphogenesis and the genetic basis of cotton pigment glands is reviewed,for providing a theoretical basis for cultivating cotton with the ideal pigment gland trait.
文摘Cellulose synthesis could play an important rolein determining certain aspects of cotton fiberquality,and despite it’s abundance in nature,little is known about the biosynthesis of thispolymer.Recent advances in understanding thesynthesis of cellulose come from the analysis ofArabidopsis thaliana cellulose-deficient mutants,and the isolation of a number of genes
基金supported by the Young Scientists Fund of the National Natural Science Foundation of China(32101797)Central Public-interest Scientific Institution Basal Research Fund(No.1610162023020).
文摘Background Cotton is extremely affected by severe natural stresses.Drought is one of the most serious abiotic stress that adversely influences cotton growth,productivity,and fiber quality.Previous studies indicate that basic leucinezipper(bZIP)transcription factors are involved in the response of plants to various stresses.However,the molecular function and regulatory mechanism of GhVIP1 in response to drought stress are still unknown.Results In this research,GhVIP1 was cloned from a drought-tolerant variety.Expression of GhVIP1 was up-regulated in response to multiple abiotic stresses,especially under drought stress.And GhVIP1 was highly expressed in the root,stem,and 10 days post-anthesis ovule.Inhibiting the expression of GhVIP1 in cotton using the virus-induced gene silencing method resulted in higher electrical conductivity in leaves,but lower water content under drought stress compared with the WT plant.Overexpression of GhVIP1 in Arabidopsis enhanced plant drought tolerance through increasing the seed germination rate and improving the development of root.The exogenous expression of GhVIP1 up-regulated the transcription of genes associated with drought response and proline biosynthesis during drought stress in Arabidopsis.Conclusion In summary,these results indicated that GhVIP1 played a positive role in plants’response to drought stress.The use of GhVIP1 via modern biotechnology might facilitate the improvement of drought tolerance in cotton cultivars.
文摘The developing cotton fiber is considered as amodel system for studying the function ofmicrotubules in plant cell elongation and cellwall biosynthesis.It has been shown thatmicrotubules undergo active dynamic changesduring fiber development.At the molecularlevel,Dixon et al demonstrated that two alpha-tubulin and two beta-tubulin
文摘Cotton fibers are single-cell trichomes derivedfrom the epidermis of cotton ovules.Analysis oftranscripts in developing fiber cells may providevaluable information to understand mechanismsregulation fiber initiation and growth,as well asto cotton breeding.We have made an array ofmore than 5000 clones isolated from a cottonovule(and the fiber)cDNA library.
文摘所属学科：生物物理学、生物化学及分子生物学,细胞生物学,生物技术与生物工程开始日期：2017-03-22结束日期：2017-03-24所在国家：英国所在城市：英国具体地点：Warwick,U.K.主办单位：Royal Society of Chemistry联系电话：＋44（0）1223 434048会议网站：http：//www.rsc.
文摘1 Du M F,Liu X G,Liu X M,Yin X M,Han S Y,Song Q S,An S H.Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori.Sci Rep,2015,DOI：10.1038/srep08110题目甘油-3-磷酸酰基转移酶参与家蚕性信息素的生物合成过程摘要昆虫雌蛾通过释放性信息素吸引雄蛾与之交配。
