Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendo...Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendorff model and to discuss the mechanisms. SD rat were randomly divided into 4groups: control group(perfused with KH buffer),10^(-4)mol·L^(-1),5×10^(-4)mol·L^(-1),and 10^(-3)mol·L^(-1)(perfused with betaine in different concentration respectively for 10 min before ischemia followed by KH buffer perfused). During the experiment we detected LVEDP,LVDP,dp/dt,and store the tissue to do western blot and TTC staining. The result showed that in control group the recovery rate is 19.6%,after pretreatment of betaine the recovery rate all increased significantly and in a dose-dependent manner. The LVEDP is markedly decreased in betaine treatment groups compare to control group. Coronary flow only in highest betaine treatment group(10^(-3)mol·L^-) is markedly increased compare to control group,other two groups have no change. Western blotting showed that SOD level is significantly increased in betaine treatment group compare to control group and in a dose dependent manner. TTC staining suggested that infarction size is reduced in betaine treatment group compare to control group. Above all,the results suggest that betaine have protect effect on I/R injury in heart and this effect may relate to ROS signaling pathway.展开更多
OBJECTIVE To observe the effects of betaine on learning memory in SHR rats through behavioral experiments,and then to investigate the mechanisms of betaine to improve the cognitive impairment caused by hyperten⁃sion.M...OBJECTIVE To observe the effects of betaine on learning memory in SHR rats through behavioral experiments,and then to investigate the mechanisms of betaine to improve the cognitive impairment caused by hyperten⁃sion.METHODS 4-month-old male SHR rats were randomly divided into 4 groups,including betaine 10,30 and 100 mg·kg-1 groups and SHR group,and administered by gavage,10 mL·kg-1 once a day for 4 weeks.The control group was WKY rats,and the same amount of saline was given by gavage.The learning and memory behaviors of the rats were analyzed and evaluated through behavioral testing.RESULTS The results of Morris water maze showed that persistent hypertension decreased the time in the target quadrant and the number of platform crossing of the rats,and the intervention with different con⁃centrations of betaine increased the time in the target quadrant and the number of platform crossing of SHR in a dose-dependent manner.Then,persistent hypertension increased the escape latency of rats,and betaine inhibited this change.The results of new object recognition showed that the recognition index of SHR group decreased,and the recognition index of SHR rats increased with different concentrations of betaine intervention in a dose-dependent manner.The Y-maze results showed that persistent hypertension decreased the alternation in rats,and the alternation in SHR rats increased after the intervention with different concentrations of betaine,and in a dose-dependent manner.CON⁃CLUSION Persistent hypertension can cause learning memory impairment in rats.Betaine intervention has an ameliorative effect on the reduction of learning memory ability in rats caused by hypertension.展开更多
Many plants accumulate compatible solutes in response to the imposition of environmental stresses.Glycine betaine, which is one of compatible solutes in cell of plants,has been shown to have surviving ability for plan...Many plants accumulate compatible solutes in response to the imposition of environmental stresses.Glycine betaine, which is one of compatible solutes in cell of plants,has been shown to have surviving ability for plant from salt stress.Effect of glycine betaine on improving plant salt resistance was discussed in plants under salt stress.The accumulation of glycine betaine protects plants against the damaging effects of stress.Strategies of glycine betaine against the damaging effects of stress were analyzed to clarify the roles of glycine betaine in salt stress tolerance of plants.展开更多
Background:Today,stress moderators are employed to mitigate crop loss due to the adverse effects of environmental stress.The current research aimed to investigate the impacts of time and stress moderator types on agro...Background:Today,stress moderators are employed to mitigate crop loss due to the adverse effects of environmental stress.The current research aimed to investigate the impacts of time and stress moderator types on agro-physiological responses of cotton on conventional and double-cropping systems during 2017 and 2018 under saline conditions.A split-plot factorial experiment was carried out in a randomized complete block design with three replications.Cultivation system[conventional(recommended planting date)and double-cropping systems(sowing after harvest of wheat)]were considered as the main plots,and stress moderator type at four levels[water control,2 mmol·L^(-1) Salicylic acid(SA),100 mmol·L^(-1) Glycine betaine(GB),and 100μmol·L^(-1) sodium nitroprusside(SNP)]and application time(flowering and flowering+bolling stages)were regarded as subplots.Results:Plant height,reproductive branch number,the number of bolls,10-boll weight,1000-seed weight,biological yield,seed cotton yield,lint yield,chlorophyll a,chlorophyll b,carotenoids,total chlorophyll,sodium,potassium,and proline content were less in double-cropping system comparing with conventional system.Spraying with the stress moderators alleviated soil salinity effects on yield,yield components,and biochemical traits of cotton.SNP spraying led to maximum plant height,branch number,the number of bolls per plant,10-boll weight and seed cotton yield.SA spraying yielded the highest 1000-seed weight,biological yield,lint percentage and lint yield.The highest chlorophyll a,chlorophyll b,carotenoids,and total chlorophyll content resulted from SNP spraying.Yield,yield components,and biochemical traits did not respond to the stress moderator types in double-cropping system.However,the highest chlorophyll a,carotenoids,proline content,the number of bolls per plant,and seed cotton yield resulted from SNP spraying in conventional system.No statistically significant differences were observed between spraying with SNP and SA in most studied traits.Conclusions:The results suggest that the optimum cotton planting time and SNP spraying could be recommended for producing the most suitable yield under saline conditions.Highlights:External application of stress modulators increases salinity stress tolerance.Spraying with sodium nitroprusside has more moderating effect.Agro-physiological response of cotton to moderators is stronger in early sowing.Maximum seed cotton yield was achieved at early sowing and spraying sodium nitroprusside.Delayed cultivation reduces cotton yield.展开更多
基金The project supported by National Natural Science Foundation of China(81400182)National Training Programs of Innovation and Entrepreneurship for Undergraduates(201610439129)
文摘Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendorff model and to discuss the mechanisms. SD rat were randomly divided into 4groups: control group(perfused with KH buffer),10^(-4)mol·L^(-1),5×10^(-4)mol·L^(-1),and 10^(-3)mol·L^(-1)(perfused with betaine in different concentration respectively for 10 min before ischemia followed by KH buffer perfused). During the experiment we detected LVEDP,LVDP,dp/dt,and store the tissue to do western blot and TTC staining. The result showed that in control group the recovery rate is 19.6%,after pretreatment of betaine the recovery rate all increased significantly and in a dose-dependent manner. The LVEDP is markedly decreased in betaine treatment groups compare to control group. Coronary flow only in highest betaine treatment group(10^(-3)mol·L^-) is markedly increased compare to control group,other two groups have no change. Western blotting showed that SOD level is significantly increased in betaine treatment group compare to control group and in a dose dependent manner. TTC staining suggested that infarction size is reduced in betaine treatment group compare to control group. Above all,the results suggest that betaine have protect effect on I/R injury in heart and this effect may relate to ROS signaling pathway.
基金National Natural Science Foundation of China(81400182)and Natural Science Foundation of Shandong Province(BS2014YY045)。
文摘OBJECTIVE To observe the effects of betaine on learning memory in SHR rats through behavioral experiments,and then to investigate the mechanisms of betaine to improve the cognitive impairment caused by hyperten⁃sion.METHODS 4-month-old male SHR rats were randomly divided into 4 groups,including betaine 10,30 and 100 mg·kg-1 groups and SHR group,and administered by gavage,10 mL·kg-1 once a day for 4 weeks.The control group was WKY rats,and the same amount of saline was given by gavage.The learning and memory behaviors of the rats were analyzed and evaluated through behavioral testing.RESULTS The results of Morris water maze showed that persistent hypertension decreased the time in the target quadrant and the number of platform crossing of the rats,and the intervention with different con⁃centrations of betaine increased the time in the target quadrant and the number of platform crossing of SHR in a dose-dependent manner.Then,persistent hypertension increased the escape latency of rats,and betaine inhibited this change.The results of new object recognition showed that the recognition index of SHR group decreased,and the recognition index of SHR rats increased with different concentrations of betaine intervention in a dose-dependent manner.The Y-maze results showed that persistent hypertension decreased the alternation in rats,and the alternation in SHR rats increased after the intervention with different concentrations of betaine,and in a dose-dependent manner.CON⁃CLUSION Persistent hypertension can cause learning memory impairment in rats.Betaine intervention has an ameliorative effect on the reduction of learning memory ability in rats caused by hypertension.
基金Supported by 11th Five Years Key Programs for Science and Technology Development of China(2006BAD03A0306)
文摘Many plants accumulate compatible solutes in response to the imposition of environmental stresses.Glycine betaine, which is one of compatible solutes in cell of plants,has been shown to have surviving ability for plant from salt stress.Effect of glycine betaine on improving plant salt resistance was discussed in plants under salt stress.The accumulation of glycine betaine protects plants against the damaging effects of stress.Strategies of glycine betaine against the damaging effects of stress were analyzed to clarify the roles of glycine betaine in salt stress tolerance of plants.
文摘Background:Today,stress moderators are employed to mitigate crop loss due to the adverse effects of environmental stress.The current research aimed to investigate the impacts of time and stress moderator types on agro-physiological responses of cotton on conventional and double-cropping systems during 2017 and 2018 under saline conditions.A split-plot factorial experiment was carried out in a randomized complete block design with three replications.Cultivation system[conventional(recommended planting date)and double-cropping systems(sowing after harvest of wheat)]were considered as the main plots,and stress moderator type at four levels[water control,2 mmol·L^(-1) Salicylic acid(SA),100 mmol·L^(-1) Glycine betaine(GB),and 100μmol·L^(-1) sodium nitroprusside(SNP)]and application time(flowering and flowering+bolling stages)were regarded as subplots.Results:Plant height,reproductive branch number,the number of bolls,10-boll weight,1000-seed weight,biological yield,seed cotton yield,lint yield,chlorophyll a,chlorophyll b,carotenoids,total chlorophyll,sodium,potassium,and proline content were less in double-cropping system comparing with conventional system.Spraying with the stress moderators alleviated soil salinity effects on yield,yield components,and biochemical traits of cotton.SNP spraying led to maximum plant height,branch number,the number of bolls per plant,10-boll weight and seed cotton yield.SA spraying yielded the highest 1000-seed weight,biological yield,lint percentage and lint yield.The highest chlorophyll a,chlorophyll b,carotenoids,and total chlorophyll content resulted from SNP spraying.Yield,yield components,and biochemical traits did not respond to the stress moderator types in double-cropping system.However,the highest chlorophyll a,carotenoids,proline content,the number of bolls per plant,and seed cotton yield resulted from SNP spraying in conventional system.No statistically significant differences were observed between spraying with SNP and SA in most studied traits.Conclusions:The results suggest that the optimum cotton planting time and SNP spraying could be recommended for producing the most suitable yield under saline conditions.Highlights:External application of stress modulators increases salinity stress tolerance.Spraying with sodium nitroprusside has more moderating effect.Agro-physiological response of cotton to moderators is stronger in early sowing.Maximum seed cotton yield was achieved at early sowing and spraying sodium nitroprusside.Delayed cultivation reduces cotton yield.
