Two experiments were conducted to study the effects of variety and maturity stage on chemical composition and in vitro dry matter degradability of oat and barley forages. In exp1, four oat varieties and one barley var...Two experiments were conducted to study the effects of variety and maturity stage on chemical composition and in vitro dry matter degradability of oat and barley forages. In exp1, four oat varieties and one barley variety were harvested at three maturity stages: stage 1, Two weeks before stage 2; stage 2, Mid-dough for oat and early-dough for barley; stage 3, One week after stage 2. All samples were analyzed for DM, CP, ether extract, ash, ADF(acid detergent fiber), NDF(neutral detergent fiber), ADL(acid detergent lignin), NDICP(neutral detergent insoluble crude protein) and IVDMD(In vitro dry matter digestibility). There was no difference in chemical components and IVDMD among oat variety. But maturity stage had a profound influence on some nutrient contents and IVDMD. With maturity advancing, CP decreased from15.5% to 11.03% and from 14.7% to 10.9% at stage 1 and stage 2 for oat and for barley, respectively, and IVDMD decreased slowly from 72.0% to 64.9% and from 73.5% to 68.8% for oat and for barley, respectively. For fiber fractions, NDF declined before stage 2 and then level off after stage 2, while ADF changed little throughout maturation. The ADL trended to increase with increased maturity. The results from the present experiment revealed that Bailor has relatively greater IVDMD value and kept lower ADL content than any other cultivars. The observed variation in chemical composition and ruminal digestibility among various oat cultivars would allow breeders choose suitable oat forages for cattle diet. In experiment. 2, the DaisyⅡ incubator was used to compare IVDMD values of forages of seven oat cultivars using rumen fluid or faecal liquor as the inoculum source. There was a strong linear relationship between the two estimates (R 2 = 0.809; P < 0.001), which indicated that fecal liquor method has the potential to be used instead of rumen fluid for estimation of in vitro digestibility of forage. It is suggested the method for determination of IVDMD using faecal liquor was appropriate for batch operation with precision and efficiency.展开更多
In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15℃ and 12 h-...In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15℃ and 12 h-light per day for 7 days, and then subjected to herbicide treatment, prometryn （with the concentrations of 0, 0.15%, 0.30%, and 0.45%） or isoproturon （with the concentrations of 0, 0.30%, 0.60%, and 0.90%）, and the SOD activity, the CAT activity, the POD activity, the MDA content, proline content, soluble protein content, electrical conductivity, and the rate of O2 were determined and analyzed. The results showed that the low-temperature was the most important, and the treatment-time of low-temperature was another significant influencing factor on the physiological and biochemical indices of barley seedlings. However, all of the physiological and biochemical indices determined were not affected by the kinds of herbicides and herbicide concentrations, and the SOD was stable and should play the more prominent role on extracting of free radicals according to the stepwise regression and correlation. The herbicide concentrations should be increased in the future research for truly reflecting the effects of the herbicide concentration on the physiological and biochemical indices of barley seedlings.展开更多
The purpose of this study was to explore the quality of the Tibetan native hulless barley variety in depth and to evaluate the characteristics of its processing quality using ratio analysis.For this study,10 native ba...The purpose of this study was to explore the quality of the Tibetan native hulless barley variety in depth and to evaluate the characteristics of its processing quality using ratio analysis.For this study,10 native barley varieties were chosen with the detection of 24 quality indexes in order to build a system of comprehensive evaluation.The results of the factor analysis indicated that seven common factors with an eigenvalue greater than 1 were extracted,cumulatively accounting for 96.21%of the total variance.The first common factor,including ASP,GLU,SER,GLY,ARG,TYR and CYS contents,accounted for 33.82% of the variance.The second common factor,including ash,the total starch,soluble fiber,VB_(3),Cu,Mn,Na and beta-glucan contents,accounted for 19.46%of the variance.The third common factor,including the total dietary fiber,α-VE,K,Zn and glutelin.The fourth common factor,including B,Ba and prolamin,explained the barley starch character of the rheological property.The fifth common factor included crude fiber.The sixth and the seventh common factors did not account for a substantial amount of variance.According to the comprehensive evaluation model,the score consequence was as the following:Zangqing25>Pengnaigabu>Lhasa changhei>2004Qing21>Lhasa duanbai>Liangamu>Zhikonggaxia>lianmubai>Jiangreejiu>Longzihei.展开更多
Root C and root-released C are closely related to soil organic matter content and mechanistic simulation modeling has proven to be useful for studying root and soil organic C dynamics in plant-soil ecosystems. A compu...Root C and root-released C are closely related to soil organic matter content and mechanistic simulation modeling has proven to be useful for studying root and soil organic C dynamics in plant-soil ecosystems. A computer model was designed in this study to simulate the dynamics of root C and root released C decomposition in situ and the dynamics of different forms of C in soil under two barley cultivars （Abee and Samson）. The results showed that on the 15th day, about 48% of the total 14C fixed in roots was respired for Abee and 42% for Samson. This indicated that the turnover rate of root ^14C of Abee was higher than that of Samson. The percentage of water-soluble organic ^14C, active microbial ^14C and stable ^14C over the total fixed ^14C were not different between two barley cultivars. From the analysis of the model for two barley cultivars, the total ~4C transformed into different soil pools （excluding CO：-C and root C pools） for the two barley cultivars was similar （26% for Abee and 25% for Samson）, but the difference of ^14C remaining in soil between the two barley cultivars was mainly because of the difference of ^14C remaining in roots which have not been yet decomposed. Some of the information which could not be measured in the laboratory conditions was obtained in this studv.展开更多
Barley Fusarium head blight(FHB),caused by species of the Fusarium fungus,is a devastating disease that is reemerging worldwide in recent years.In this study,a novel gene,HvORG4,was cloned from barley by using cDNA li...Barley Fusarium head blight(FHB),caused by species of the Fusarium fungus,is a devastating disease that is reemerging worldwide in recent years.In this study,a novel gene,HvORG4,was cloned from barley by using cDNA library and suppression subtractive hybridization(SSH) library strategies.The SSH library and cDNA library were constructed from the Chinese barley cultivar Jing02-461(resistance to FHB) infected by Fusarium graminearum isolate Huanggang-1.For the SSH analysis,more than 120 differentially expressed cDNAs were identified and sequenced.One of them showed high homology to the AtORG4 gene and was used as a probe to screen the cDNA library of Jing02-461.Six positive clones were identified and one of them contained a full-length cDNA,which was named HvORG4.Sequence analysis showed that HvORG4 encoded a deduced basic protein of 197 amino acids.Northern blotting analysis showed that HvORG4 was constitutively expressed in root and stalk,not in leaf or spike,and strongly induced in barley spikelets in response to infection with F.graminearum isolate Huanggang-1.Its homology and expression profile suggest that the HvORG4 might function as a transcription factor,playing an important role in signal transduction pathway for defense against FHB in barley.展开更多
Barley varieties Aramir and Golf were crossed with Hordeum bulbosum. Haploid embryos as well as diploid embryos of their parents were cultured for callus induction. Calli induced were irradiated in doses of 0, 5, 10, ...Barley varieties Aramir and Golf were crossed with Hordeum bulbosum. Haploid embryos as well as diploid embryos of their parents were cultured for callus induction. Calli induced were irradiated in doses of 0, 5, 10, 25 and 50 GY. Results showed that seed set and callus induction frequency of haploid and diploid embryos in combination Aramir × bulbosum were higher than that in Golf x bulbosum, Diploid and haploid calli from embryos involved Golf were more sensitive than that involved Aramir. Acorrding to dead callus frequency and regeneration frequency, the optimum dose for irradiating barley callus was above 5 GY, less than 10 GY.展开更多
Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease c...Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.展开更多
文摘Two experiments were conducted to study the effects of variety and maturity stage on chemical composition and in vitro dry matter degradability of oat and barley forages. In exp1, four oat varieties and one barley variety were harvested at three maturity stages: stage 1, Two weeks before stage 2; stage 2, Mid-dough for oat and early-dough for barley; stage 3, One week after stage 2. All samples were analyzed for DM, CP, ether extract, ash, ADF(acid detergent fiber), NDF(neutral detergent fiber), ADL(acid detergent lignin), NDICP(neutral detergent insoluble crude protein) and IVDMD(In vitro dry matter digestibility). There was no difference in chemical components and IVDMD among oat variety. But maturity stage had a profound influence on some nutrient contents and IVDMD. With maturity advancing, CP decreased from15.5% to 11.03% and from 14.7% to 10.9% at stage 1 and stage 2 for oat and for barley, respectively, and IVDMD decreased slowly from 72.0% to 64.9% and from 73.5% to 68.8% for oat and for barley, respectively. For fiber fractions, NDF declined before stage 2 and then level off after stage 2, while ADF changed little throughout maturation. The ADL trended to increase with increased maturity. The results from the present experiment revealed that Bailor has relatively greater IVDMD value and kept lower ADL content than any other cultivars. The observed variation in chemical composition and ruminal digestibility among various oat cultivars would allow breeders choose suitable oat forages for cattle diet. In experiment. 2, the DaisyⅡ incubator was used to compare IVDMD values of forages of seven oat cultivars using rumen fluid or faecal liquor as the inoculum source. There was a strong linear relationship between the two estimates (R 2 = 0.809; P < 0.001), which indicated that fecal liquor method has the potential to be used instead of rumen fluid for estimation of in vitro digestibility of forage. It is suggested the method for determination of IVDMD using faecal liquor was appropriate for batch operation with precision and efficiency.
基金Supported by the National Natural Science Foundation of China (31000712)
文摘In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15℃ and 12 h-light per day for 7 days, and then subjected to herbicide treatment, prometryn （with the concentrations of 0, 0.15%, 0.30%, and 0.45%） or isoproturon （with the concentrations of 0, 0.30%, 0.60%, and 0.90%）, and the SOD activity, the CAT activity, the POD activity, the MDA content, proline content, soluble protein content, electrical conductivity, and the rate of O2 were determined and analyzed. The results showed that the low-temperature was the most important, and the treatment-time of low-temperature was another significant influencing factor on the physiological and biochemical indices of barley seedlings. However, all of the physiological and biochemical indices determined were not affected by the kinds of herbicides and herbicide concentrations, and the SOD was stable and should play the more prominent role on extracting of free radicals according to the stepwise regression and correlation. The herbicide concentrations should be increased in the future research for truly reflecting the effects of the herbicide concentration on the physiological and biochemical indices of barley seedlings.
基金Supported by Chnia Agriculture Research Systemthe Scientific Research Fund of the Key Technology and Research and Development of Barley Characteristic Agricultural Products Processing(XZ201901NA04)Development and Industrialization Application of Xizang Highland Barley Baijiu(XZ202001ZY0017N)。
文摘The purpose of this study was to explore the quality of the Tibetan native hulless barley variety in depth and to evaluate the characteristics of its processing quality using ratio analysis.For this study,10 native barley varieties were chosen with the detection of 24 quality indexes in order to build a system of comprehensive evaluation.The results of the factor analysis indicated that seven common factors with an eigenvalue greater than 1 were extracted,cumulatively accounting for 96.21%of the total variance.The first common factor,including ASP,GLU,SER,GLY,ARG,TYR and CYS contents,accounted for 33.82% of the variance.The second common factor,including ash,the total starch,soluble fiber,VB_(3),Cu,Mn,Na and beta-glucan contents,accounted for 19.46%of the variance.The third common factor,including the total dietary fiber,α-VE,K,Zn and glutelin.The fourth common factor,including B,Ba and prolamin,explained the barley starch character of the rheological property.The fifth common factor included crude fiber.The sixth and the seventh common factors did not account for a substantial amount of variance.According to the comprehensive evaluation model,the score consequence was as the following:Zangqing25>Pengnaigabu>Lhasa changhei>2004Qing21>Lhasa duanbai>Liangamu>Zhikonggaxia>lianmubai>Jiangreejiu>Longzihei.
文摘Root C and root-released C are closely related to soil organic matter content and mechanistic simulation modeling has proven to be useful for studying root and soil organic C dynamics in plant-soil ecosystems. A computer model was designed in this study to simulate the dynamics of root C and root released C decomposition in situ and the dynamics of different forms of C in soil under two barley cultivars （Abee and Samson）. The results showed that on the 15th day, about 48% of the total 14C fixed in roots was respired for Abee and 42% for Samson. This indicated that the turnover rate of root ^14C of Abee was higher than that of Samson. The percentage of water-soluble organic ^14C, active microbial ^14C and stable ^14C over the total fixed ^14C were not different between two barley cultivars. From the analysis of the model for two barley cultivars, the total ~4C transformed into different soil pools （excluding CO：-C and root C pools） for the two barley cultivars was similar （26% for Abee and 25% for Samson）, but the difference of ^14C remaining in soil between the two barley cultivars was mainly because of the difference of ^14C remaining in roots which have not been yet decomposed. Some of the information which could not be measured in the laboratory conditions was obtained in this studv.
文摘Barley Fusarium head blight(FHB),caused by species of the Fusarium fungus,is a devastating disease that is reemerging worldwide in recent years.In this study,a novel gene,HvORG4,was cloned from barley by using cDNA library and suppression subtractive hybridization(SSH) library strategies.The SSH library and cDNA library were constructed from the Chinese barley cultivar Jing02-461(resistance to FHB) infected by Fusarium graminearum isolate Huanggang-1.For the SSH analysis,more than 120 differentially expressed cDNAs were identified and sequenced.One of them showed high homology to the AtORG4 gene and was used as a probe to screen the cDNA library of Jing02-461.Six positive clones were identified and one of them contained a full-length cDNA,which was named HvORG4.Sequence analysis showed that HvORG4 encoded a deduced basic protein of 197 amino acids.Northern blotting analysis showed that HvORG4 was constitutively expressed in root and stalk,not in leaf or spike,and strongly induced in barley spikelets in response to infection with F.graminearum isolate Huanggang-1.Its homology and expression profile suggest that the HvORG4 might function as a transcription factor,playing an important role in signal transduction pathway for defense against FHB in barley.
文摘Barley varieties Aramir and Golf were crossed with Hordeum bulbosum. Haploid embryos as well as diploid embryos of their parents were cultured for callus induction. Calli induced were irradiated in doses of 0, 5, 10, 25 and 50 GY. Results showed that seed set and callus induction frequency of haploid and diploid embryos in combination Aramir × bulbosum were higher than that in Golf x bulbosum, Diploid and haploid calli from embryos involved Golf were more sensitive than that involved Aramir. Acorrding to dead callus frequency and regeneration frequency, the optimum dose for irradiating barley callus was above 5 GY, less than 10 GY.
基金Supported by the National Items of Research and Industrial Development of Transgenic Plants(J99-B-013)
文摘Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.
