【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechan...【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechanism of A.subulatus Michx.【Method】After cultured for 24 d under the action of Hoagland nutrient solution and gradient Cd concentrations(0,30,60 and 90 mg/L),A.subulatus Michx.were harvested,and its leaf,stem and root were treated by differential centrifugation,chemical reagent extraction,and digested with graphite digester,respectively,then the Cd content in the root,stem and leaf were determined by atomic absorption spectroscopy.【Result】The experimental results indicated that the bioaccumulation capacity of Cd in A.subulatus Michx.was root>stem>leaf,and the maximum Cd concentration in the root,stem and leaf of A.subulatus Michx.were 130.74,78.69 and 56.62 mg/kg(fresh matter),respectively.Most of Cd stored in the cell wall and the soluble fractions of the root and leaf of A.subulatus Michx.,with only a smaller portion Cd in organelle fraction.Analysis result of subcellular Cd content showed that 52.27%-58.61%of Cd for root was mainly stored in the soluble fraction,but 42.10%-63.28%of Cd for leaf was mainly stored in the cell wall fraction.The concentration of pectates and protein integrated-Cd was higher in the root and leaf compared to other chemical forms Cd.Pectates and protein integrated-Cd was the main chemical forms Cd in the root and leaf of A.subulatus Michx.,and their percentages were 68.91%-74.80%and 57.38%-83.80%,respectively.Cd treatment could significantly increase the proportion of water-soluble organic acid Cd from 13.64%to 22.72%in root and undissolved phosphate Cd from 10.02%to 32.78%in leaf with increasing Cd concentration in the culture medium.【Conclusion】The root,stem and leaf of A.subulatus Michx.has strong bioaccumulation capacity to Cd,Cd is primarily stored in the soluble fractions of the root and cell wall fractions of the leaf,and less toxic pectates and protein integrated-Cd is the main chemical forms Cd in the root and leaf of A.subulatus Michx.,this might be the main mechanism of Cd tolerance in A.subulatus Michx.展开更多
遥感卫星的多光谱数据应用于找矿已取得显著成效,2004年7月中国卫星地面站开始提供ASTER(Advanced Space-borne Thermal Emission and Reflection Radiometer,高级星载热发射反照辐射计)数据,因涵盖波长范围宽[VNIR(Visible and Near In...遥感卫星的多光谱数据应用于找矿已取得显著成效,2004年7月中国卫星地面站开始提供ASTER(Advanced Space-borne Thermal Emission and Reflection Radiometer,高级星载热发射反照辐射计)数据,因涵盖波长范围宽[VNIR(Visible and Near Infra-red)、SWIR(Short wave-length Infra-red)、TIR(Thermal Infra-red)]、波段多(14个波段)、性价比合理等因素,ASTER数据的研究迅速发展。长久以来,对覆盖区进行蚀变遥感异常信息提取一直是遥感找矿的关注点之一。笔者等利用ASTER数据对浅覆盖区——包古图斑岩铜矿的Ⅱ号、Ⅴ号斑岩体进行蚀变遥感异常提取,提取的蚀变异常与野外地质情况吻合性好。分别提取了光谱特征谱带差异明显的2组蚀变矿物的异常信息:第一组是蒙脱石、埃洛石、伊利石与绢云母;第二组是方解石、黑云母与绿泥石。提出了需要进一步工作的异常靶区。展开更多
基金Guangxi Natural Science Foundation(2024GXNSFAA010469,2021GXNSFBA196028)Science and Technology Development Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021YT137,Guinongke 2022JM86)。
文摘【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechanism of A.subulatus Michx.【Method】After cultured for 24 d under the action of Hoagland nutrient solution and gradient Cd concentrations(0,30,60 and 90 mg/L),A.subulatus Michx.were harvested,and its leaf,stem and root were treated by differential centrifugation,chemical reagent extraction,and digested with graphite digester,respectively,then the Cd content in the root,stem and leaf were determined by atomic absorption spectroscopy.【Result】The experimental results indicated that the bioaccumulation capacity of Cd in A.subulatus Michx.was root>stem>leaf,and the maximum Cd concentration in the root,stem and leaf of A.subulatus Michx.were 130.74,78.69 and 56.62 mg/kg(fresh matter),respectively.Most of Cd stored in the cell wall and the soluble fractions of the root and leaf of A.subulatus Michx.,with only a smaller portion Cd in organelle fraction.Analysis result of subcellular Cd content showed that 52.27%-58.61%of Cd for root was mainly stored in the soluble fraction,but 42.10%-63.28%of Cd for leaf was mainly stored in the cell wall fraction.The concentration of pectates and protein integrated-Cd was higher in the root and leaf compared to other chemical forms Cd.Pectates and protein integrated-Cd was the main chemical forms Cd in the root and leaf of A.subulatus Michx.,and their percentages were 68.91%-74.80%and 57.38%-83.80%,respectively.Cd treatment could significantly increase the proportion of water-soluble organic acid Cd from 13.64%to 22.72%in root and undissolved phosphate Cd from 10.02%to 32.78%in leaf with increasing Cd concentration in the culture medium.【Conclusion】The root,stem and leaf of A.subulatus Michx.has strong bioaccumulation capacity to Cd,Cd is primarily stored in the soluble fractions of the root and cell wall fractions of the leaf,and less toxic pectates and protein integrated-Cd is the main chemical forms Cd in the root and leaf of A.subulatus Michx.,this might be the main mechanism of Cd tolerance in A.subulatus Michx.
文摘遥感卫星的多光谱数据应用于找矿已取得显著成效,2004年7月中国卫星地面站开始提供ASTER(Advanced Space-borne Thermal Emission and Reflection Radiometer,高级星载热发射反照辐射计)数据,因涵盖波长范围宽[VNIR(Visible and Near Infra-red)、SWIR(Short wave-length Infra-red)、TIR(Thermal Infra-red)]、波段多(14个波段)、性价比合理等因素,ASTER数据的研究迅速发展。长久以来,对覆盖区进行蚀变遥感异常信息提取一直是遥感找矿的关注点之一。笔者等利用ASTER数据对浅覆盖区——包古图斑岩铜矿的Ⅱ号、Ⅴ号斑岩体进行蚀变遥感异常提取,提取的蚀变异常与野外地质情况吻合性好。分别提取了光谱特征谱带差异明显的2组蚀变矿物的异常信息:第一组是蒙脱石、埃洛石、伊利石与绢云母;第二组是方解石、黑云母与绿泥石。提出了需要进一步工作的异常靶区。
