采用生长曲线指导的富集培养法,从生活污水排放渠中分离筛选出高效异养硝化细菌,并对其多样性进行了分析。结果显示,从分离得到的27株异养硝化细菌中筛选出6株高效菌株Ni1-2、Ni1-8、Ni2-5、Ni2-7、Ni3-1和Ni3-4,其48 h氨氮去除率分别为...采用生长曲线指导的富集培养法,从生活污水排放渠中分离筛选出高效异养硝化细菌,并对其多样性进行了分析。结果显示,从分离得到的27株异养硝化细菌中筛选出6株高效菌株Ni1-2、Ni1-8、Ni2-5、Ni2-7、Ni3-1和Ni3-4,其48 h氨氮去除率分别为88.9%、76.6%、87.7%、93.1%、99.2%和91.4%;结合菌落形态、革兰氏染色反应、扫描电镜观察和16S r DNA序列分析,发现菌株的种类较为丰富,初步确定Ni1-2和Ni1-8为节杆菌属(Arthrobacter),Ni2-5和Ni3-1为产碱杆菌属(Alcaligenes),Ni2-7为无色杆菌属(Achromobacter),Ni3-4为芽孢杆菌属(Bacillus)。该结果可为高效异养硝化菌的分离筛选及其多样性分析提供参考。展开更多
研究了海洋细菌原玻璃蝇节杆菌(Arthrobacter protophormiae)对水溶液中Pb(Ⅱ)的吸附性能,探讨了溶液酸碱度、温度、菌体浓度和接触时间等因素对去除效率的影响。利用原子力显微镜技术探测吸附前后细胞表面的变化。结果表明,该海洋菌体...研究了海洋细菌原玻璃蝇节杆菌(Arthrobacter protophormiae)对水溶液中Pb(Ⅱ)的吸附性能,探讨了溶液酸碱度、温度、菌体浓度和接触时间等因素对去除效率的影响。利用原子力显微镜技术探测吸附前后细胞表面的变化。结果表明,该海洋菌体吸附铅离子的最佳酸碱度为p H 6,去除铅离子的最佳温度为55℃,且增大菌体浓度有利于铅离子的去除,准二级动力学模型较好地拟合了动力学数据。铅离子的吸附导致细菌细胞表面产生显著变化。展开更多
采用富集培养方法在低温条件下从生活垃圾土壤中分离得到1株能够降解纤维素细菌,命名为FLX-1。经形态学、生理生化学和分子生物学16S r DNA序列分析,将菌株FLX-1鉴定为节杆菌属(Arthrobacter sp.)细菌。为探索菌株FLX-1生长特性和最佳...采用富集培养方法在低温条件下从生活垃圾土壤中分离得到1株能够降解纤维素细菌,命名为FLX-1。经形态学、生理生化学和分子生物学16S r DNA序列分析,将菌株FLX-1鉴定为节杆菌属(Arthrobacter sp.)细菌。为探索菌株FLX-1生长特性和最佳产酶条件,利用响应面分析方法考查培养时间、温度、p H和接种量对菌株FLX-1产羧甲基纤维素(Carboxymethyl cellulose,CMC)酶活特性影响情况。结果表明,菌株FLX-1最佳产酶条件为培养时间74.1 h,温度10.5℃,p H 7.1,接种量2.1%。在最佳条件下,菌株FLX-1产CMC酶活值为14.12 U·m L-1。展开更多
A bacterial strain that was capable of degrading organic sulfur (dibenzothiophene) was isolated by enrichment techniques from the petroleum-contaminated soil collected from Zhongyuan Oil Field. The strain is named ZYX...A bacterial strain that was capable of degrading organic sulfur (dibenzothiophene) was isolated by enrichment techniques from the petroleum-contaminated soil collected from Zhongyuan Oil Field. The strain is named ZYX and is gram-positive. This strain undergoes bacilus-coccus morphological change, and forms yellow-pigment glossy circular colonies with 1.5 mm in diameter on average after 2 d incubation on Luria-Bertani(LB) plates. The full-length of 16S rDNA sequence of strain ZYX was determined and analyzed. Strain ZYX is found most relative with the genus of Arthrobacter. The similarity values between ZYX and Arthrobacter sp. P2 is 99.53%. The main morphological, biochemical and physiological features of strain ZYX accord with those of Arthrobacter. It is found that the optimal initial pH for growth is about 7.0, and the optimal concentration of dibenzothiophene(DBT) for growth is 0. 10 gL. Additionally, the results show that the best carbon source and nitrogen source are glycerol and glutamine, respectively.展开更多
文摘采用生长曲线指导的富集培养法,从生活污水排放渠中分离筛选出高效异养硝化细菌,并对其多样性进行了分析。结果显示,从分离得到的27株异养硝化细菌中筛选出6株高效菌株Ni1-2、Ni1-8、Ni2-5、Ni2-7、Ni3-1和Ni3-4,其48 h氨氮去除率分别为88.9%、76.6%、87.7%、93.1%、99.2%和91.4%;结合菌落形态、革兰氏染色反应、扫描电镜观察和16S r DNA序列分析,发现菌株的种类较为丰富,初步确定Ni1-2和Ni1-8为节杆菌属(Arthrobacter),Ni2-5和Ni3-1为产碱杆菌属(Alcaligenes),Ni2-7为无色杆菌属(Achromobacter),Ni3-4为芽孢杆菌属(Bacillus)。该结果可为高效异养硝化菌的分离筛选及其多样性分析提供参考。
文摘研究了海洋细菌原玻璃蝇节杆菌(Arthrobacter protophormiae)对水溶液中Pb(Ⅱ)的吸附性能,探讨了溶液酸碱度、温度、菌体浓度和接触时间等因素对去除效率的影响。利用原子力显微镜技术探测吸附前后细胞表面的变化。结果表明,该海洋菌体吸附铅离子的最佳酸碱度为p H 6,去除铅离子的最佳温度为55℃,且增大菌体浓度有利于铅离子的去除,准二级动力学模型较好地拟合了动力学数据。铅离子的吸附导致细菌细胞表面产生显著变化。
基金Project(50621063) supported by the National Natural Science Foundation of ChinaProject(05JJ30023) supportecd by Natural Science Foundation of Hunan Province, China
文摘A bacterial strain that was capable of degrading organic sulfur (dibenzothiophene) was isolated by enrichment techniques from the petroleum-contaminated soil collected from Zhongyuan Oil Field. The strain is named ZYX and is gram-positive. This strain undergoes bacilus-coccus morphological change, and forms yellow-pigment glossy circular colonies with 1.5 mm in diameter on average after 2 d incubation on Luria-Bertani(LB) plates. The full-length of 16S rDNA sequence of strain ZYX was determined and analyzed. Strain ZYX is found most relative with the genus of Arthrobacter. The similarity values between ZYX and Arthrobacter sp. P2 is 99.53%. The main morphological, biochemical and physiological features of strain ZYX accord with those of Arthrobacter. It is found that the optimal initial pH for growth is about 7.0, and the optimal concentration of dibenzothiophene(DBT) for growth is 0. 10 gL. Additionally, the results show that the best carbon source and nitrogen source are glycerol and glutamine, respectively.
