Purpose: To establish a HPLC testing method of the content of bulk picoplatin and its impurities. Method: the separation was perform on a C18 column(4.6 mm×250 mm, 5 m) with potassium dihydrogen phosphate-aceton-...Purpose: To establish a HPLC testing method of the content of bulk picoplatin and its impurities. Method: the separation was perform on a C18 column(4.6 mm×250 mm, 5 m) with potassium dihydrogen phosphate-aceton-itrile as the mobile phase at a flow rate of 1.0 mL/min. The detecting wavelength was set at 210 nm, and the column temperature was set at 30℃. Result: in the method validation, the linear relationship modulus of picoplatin is 0.9999, the systemic precision is 0.44%, the method precision is 0.74%, the average recovery rate is 99.62%, the LOD and LOQ of picoplatin is 0.2 ng and 1.0 ng. The average resolution of picoplatin and its impurities is more than 2. Conclusion: The established method is good specificity, high sensitivity, and good repeatability which could provide scientific evidence for the quality control of picoplatin and its impurities.展开更多
In order to control the silver content in the preparation process of platinum group anti-cancer drugs, we put two kinds of color reagent to color in the production process of the platinum anti-cancer drugs by UV spect...In order to control the silver content in the preparation process of platinum group anti-cancer drugs, we put two kinds of color reagent to color in the production process of the platinum anti-cancer drugs by UV spectra measurement to control drugs production of platinum anticancer, thus we could control the silver content in the drugs so that it meets the pharmacopoeia standards of US and展开更多
为了更深入地探究花色苷在抗肝癌活性功能研究领域的全球进展及发展趋势,采用文献计量学的方法,对2003-2023年Web of Science核心数据库中收录的相关文献进行了全面的热点追踪与趋势剖析。采用VOSviewer软件,分析了花色苷抗肝癌研究的...为了更深入地探究花色苷在抗肝癌活性功能研究领域的全球进展及发展趋势,采用文献计量学的方法,对2003-2023年Web of Science核心数据库中收录的相关文献进行了全面的热点追踪与趋势剖析。采用VOSviewer软件,分析了花色苷抗肝癌研究的文献数量、发表国家、核心作者群体、研究机构分布,还深入挖掘了该领域的研究热点。结果显示,近年来关于花色苷抗肝癌活性的学术论文发表数量持续攀升,反映出该领域日益增长的研究兴趣与重要性。在地域分布上,中国与美国是这一研究领域内发表论文最为活跃的两个国家。埃及知识库与开罗大学凭借其丰富的科研成果,位列研究机构发文量榜首。通过对高频关键词的细致分类与归纳,可以清晰识别出当前研究的三大核心热点:“anthocyanins”(花色苷)、“anticancer”(抗癌)以及“apoptosis”(细胞凋亡)。这些热点不仅揭示了花色苷作为抗癌物质,特别是在抗肝癌方面的巨大潜力,也指明了未来研究的关键方向与重点。展开更多
对醋柴胡多糖(polysaccharide from vinegar-baked Bupleuri Radix,VBRBP)进行分离纯化,初步分析其结构特征并探究其联合化疗药的协同抗肝癌活性。通过水提醇沉、膜分离及纤维素层析从醋柴胡中分离纯化得到醋柴胡均一多糖,采用高效凝胶...对醋柴胡多糖(polysaccharide from vinegar-baked Bupleuri Radix,VBRBP)进行分离纯化,初步分析其结构特征并探究其联合化疗药的协同抗肝癌活性。通过水提醇沉、膜分离及纤维素层析从醋柴胡中分离纯化得到醋柴胡均一多糖,采用高效凝胶渗透色谱法、PMP柱前衍生化法、傅里叶变换红外光谱、气质联用和核磁共振波谱等方法对醋柴胡均一多糖进行结构鉴定,并测定其联合不同化疗药物对HepG2和Huh7两种肝癌细胞株的增殖抑制作用。结果表明,经过分离纯化得到的醋柴胡均一多糖5-1(polysaccharide 5-1 from vinegar-baked Bupleuri Radix,VBRBP5-1)是一种中性多糖,相对分子量为30.78 kDa,由甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成,摩尔比为1.96∶2.38∶58.48∶22.85∶14.32;主链是由1-α-D-葡萄糖、1,6-α-D-葡萄糖、1,4,6-α-D-葡萄糖、1,4-α-D-葡萄糖、1,3,6-β-D-半乳糖残基构成,支链主要含有1-β-D-半乳糖、1,4-β-D-半乳糖和1-α-L-阿拉伯糖、1,3,5-α-L-阿拉伯糖、1,5-α-L-阿拉伯糖残基片段。VBRBP5-1能增强10-羟基喜树碱、甲氨蝶呤和顺铂对肝癌细胞的抑制率,显示出协同增效作用,其中对顺铂的协同增效作用在两种肝癌细胞中均优于其他抗肿瘤药物。本研究解析了VBRBP5-1的结构并探索了其协同抗肝癌活性,为醋柴胡多糖的构效关系阐释及后续深入开发和利用提供参考。展开更多
文摘Purpose: To establish a HPLC testing method of the content of bulk picoplatin and its impurities. Method: the separation was perform on a C18 column(4.6 mm×250 mm, 5 m) with potassium dihydrogen phosphate-aceton-itrile as the mobile phase at a flow rate of 1.0 mL/min. The detecting wavelength was set at 210 nm, and the column temperature was set at 30℃. Result: in the method validation, the linear relationship modulus of picoplatin is 0.9999, the systemic precision is 0.44%, the method precision is 0.74%, the average recovery rate is 99.62%, the LOD and LOQ of picoplatin is 0.2 ng and 1.0 ng. The average resolution of picoplatin and its impurities is more than 2. Conclusion: The established method is good specificity, high sensitivity, and good repeatability which could provide scientific evidence for the quality control of picoplatin and its impurities.
文摘In order to control the silver content in the preparation process of platinum group anti-cancer drugs, we put two kinds of color reagent to color in the production process of the platinum anti-cancer drugs by UV spectra measurement to control drugs production of platinum anticancer, thus we could control the silver content in the drugs so that it meets the pharmacopoeia standards of US and
文摘为了更深入地探究花色苷在抗肝癌活性功能研究领域的全球进展及发展趋势,采用文献计量学的方法,对2003-2023年Web of Science核心数据库中收录的相关文献进行了全面的热点追踪与趋势剖析。采用VOSviewer软件,分析了花色苷抗肝癌研究的文献数量、发表国家、核心作者群体、研究机构分布,还深入挖掘了该领域的研究热点。结果显示,近年来关于花色苷抗肝癌活性的学术论文发表数量持续攀升,反映出该领域日益增长的研究兴趣与重要性。在地域分布上,中国与美国是这一研究领域内发表论文最为活跃的两个国家。埃及知识库与开罗大学凭借其丰富的科研成果,位列研究机构发文量榜首。通过对高频关键词的细致分类与归纳,可以清晰识别出当前研究的三大核心热点:“anthocyanins”(花色苷)、“anticancer”(抗癌)以及“apoptosis”(细胞凋亡)。这些热点不仅揭示了花色苷作为抗癌物质,特别是在抗肝癌方面的巨大潜力,也指明了未来研究的关键方向与重点。
