Aim Ovarian cancer has been one of most common cancers in women of all ages. Aspirin, previously known as an anti-inflammatory agent, was considered to be effective in ovarian cancer prevention and treatment. In this ...Aim Ovarian cancer has been one of most common cancers in women of all ages. Aspirin, previously known as an anti-inflammatory agent, was considered to be effective in ovarian cancer prevention and treatment. In this study, it aimed to comprehensively demonstrate the effects of aspirin on human ovarian cells HO8910 from many different stages of cell growth, differentiation and migration. Therefore, all relevant cell processes were ana- lyzed, including cell surface markers expression, cell viability, cell proliferation, cell migration, cell clustering; cell apoptosis and mitochondria membrane potential. The expression levels of relevant proteins were also provided. Finally, the potential mechanism for its inhibitory effects on cancer cells was demonstrated with cell apoptosis and cytokines regulation. Methods To explore the studies, the effects of aspirin on human ovarian cells HO8910 were comprehensively demonstrated, including cell characteristics involving cell surface markers expression CD44 ＋ and CD133 ＋, cell viability, cell proliferation, cell migration, cell clustering; cell apoptosis and mitochondria mem- brane potential; potential mechanism involving relevant proteins expression. The effects of Aspirin on human ovari- an cells were described from many different stages of cell growth, differentiation and migration. In addition, the po- tential mechanisms were demonstrated from cell apoptosis and proteins expression. Results The aspirin concentra- tions in treating cells HO8910 were range from 10, 5, 2 mmol · L^-1. VB2 was involved as controls with suggested concentration range from 40, 20, 10 mmol · L^-1. The cell surface markers percentages of CD44 ＋ and CD133 ＋ cells were decreased in both Aspirin and VB2 treated human ovarian cells （less than 80% ） , compared to that of control group （86%）. Cell proliferation viability, when the concentration of aspirin was 20 mmol · L^-1, the cell viability can be less than 40% of control and after 48 h and 72 h, the cell viability in Aspirin treated group contin- ued to be decreased, approaching 0%. For VB2, a concentration range wider than suggested concentration was in- volved in this detection （0. 25 - 100 μmol · L^-l）. After the first 24 h of VB2 treatment, the cell viability re- mained to be higher than 80 % of blank control. Colony formation of cells. The increased Aspirin concentration would lead to significantly decreased colony formation. Cell apoptosis, In aspirin treated groups, the ratio of cell ap- optosis （3%- 20% ） were greatly increased compared with control group （0.5%）, The expression of NK-KB and TNF-α,In Aspirin treated group, the expression level of TNF-α was increased with high concentration of aspirin, while the level of NK-KB was negative correlated with the level of TNF-α, which were down-regulated compared to control group. Conclusions In this study, the inhibitory effects of Aspirin on human ovarian cells HO8910 were demonstrated and analyzed. The cell viability, migration and colony formation would be suppressed after Aspirin treatment, without strict dose-effect. The cell apoptosis can be found after Aspirin treatment, as well as elevated ROS and reduced mitochondria membrane potential. The expression levels of relevant cytokines indicated down-reg- ulated NF-KB and up-regulated TNF-α, which may be the potential molecular mechanism for the tumor cell sup- pression effects of Aspirin. Cell migration with Transwell detection, The cells were treated with Aspirin and VB2 of different concentrations for 48 h （Table 2 and Figure 2 ）. Compared to the cell migration rate in control group （more than 80% ） , the cell migration was greatly reduced in both Aspirin and VB2 treated groups, which were both less than 40 %. Reactive oxygen, The reactive oxygen levels in Aspirin treated cells were greatly increased tobe 2-3 times compared to control group（Figure 3）. The dose-effect was not significant.展开更多
Objective: The present study was designed to evaluate the effect a commonly prescribed Non Steroidal Anti Inflammatory Drug(NSAID) i.e.aspirin on brush border membrane in terms of changes in the intestinal transport l...Objective: The present study was designed to evaluate the effect a commonly prescribed Non Steroidal Anti Inflammatory Drug(NSAID) i.e.aspirin on brush border membrane in terms of changes in the intestinal transport level of glucose which is monosaccharide with absolute requirement in the body and hence its absorption is directly proportional on the morphology of the intestinal mucosa.Method: Albino rats(Rattus Norvegicus) were divided into two different groups,Group I(Control),Group II(aspirin-treated,50 mg aspirin/kg of body weight).The treatment was continued for 28 days.On the 29th day after overnight fasting,intestine was removed from animals of both groups.Changes in transport of glucose-D in intestine were studied.Result: The results indicated a significant decrease in the transport of glucose-D in aspirin treated group as compared to the control group.Conclusion: Cautious use of NSAID is recommended in commonly observed symptom such as headache and to those patients who are given as a prophylaxis for thrombosis.展开更多
A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of aspirin (ASA) and its metabolite salicylic acid (SA) in human plasma has been...A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of aspirin (ASA) and its metabolite salicylic acid (SA) in human plasma has been developed and validated.The plasma ASA and SA were extracted using a liquid-liquid extraction (LLE) and the sample extract was injected onto the LC-MS/MS system.The limits of quantitation(LLOQ) for ASA and SA are both 25 ng/mL.This method allowed the reproducible and accurate quantification with the concentration rang of 25-10 000 ng/mL.This method could be applied to the quantitation aspirin and salicylic acid in human plasma.展开更多
自发性脑出血是全球范围内致死致残的重要疾病之一,尽管其外科手术治疗已日趋成熟,但相应的术后管理方案尚不明晰。其中,脑出血术后存活患者发生主要心脑及外周血管事件的风险显著增加,但现行指南尚未明确能否以及何时启动抗血小板治疗...自发性脑出血是全球范围内致死致残的重要疾病之一,尽管其外科手术治疗已日趋成熟,但相应的术后管理方案尚不明晰。其中,脑出血术后存活患者发生主要心脑及外周血管事件的风险显著增加,但现行指南尚未明确能否以及何时启动抗血小板治疗。近期发表的自发性脑出血患者神经外科手术后早期抗血小板治疗(early-start antiplatelet treatment after neurosurgery in patients with spontaneous intracerebral hemorrhage,E-start)研究表明,对于具有高缺血事件风险的脑出血患者,术后早期启动抗血小板治疗可带来显著获益,且不增加出血风险。作为国内首个脑出血围手术期管理的高级别循证证据,E-start研究有助于提高脑出血救治与管理水平,改善患者预后。展开更多
颅内动脉粥样硬化性狭窄（intracranial atherosclerotic stenosis,ICAS）是卒中的主要病因,也是卒中复发的高危因素。华法林-阿司匹林治疗症状性颅内动脉疾病试验（Warfarin and Aspirin for Symptomatic Intracranial Disease,WASID...颅内动脉粥样硬化性狭窄（intracranial atherosclerotic stenosis,ICAS）是卒中的主要病因,也是卒中复发的高危因素。华法林-阿司匹林治疗症状性颅内动脉疾病试验（Warfarin and Aspirin for Symptomatic Intracranial Disease,WASID）研究对象为50%～99%颅内血管狭窄的患者,展开更多
文摘Aim Ovarian cancer has been one of most common cancers in women of all ages. Aspirin, previously known as an anti-inflammatory agent, was considered to be effective in ovarian cancer prevention and treatment. In this study, it aimed to comprehensively demonstrate the effects of aspirin on human ovarian cells HO8910 from many different stages of cell growth, differentiation and migration. Therefore, all relevant cell processes were ana- lyzed, including cell surface markers expression, cell viability, cell proliferation, cell migration, cell clustering; cell apoptosis and mitochondria membrane potential. The expression levels of relevant proteins were also provided. Finally, the potential mechanism for its inhibitory effects on cancer cells was demonstrated with cell apoptosis and cytokines regulation. Methods To explore the studies, the effects of aspirin on human ovarian cells HO8910 were comprehensively demonstrated, including cell characteristics involving cell surface markers expression CD44 ＋ and CD133 ＋, cell viability, cell proliferation, cell migration, cell clustering; cell apoptosis and mitochondria mem- brane potential; potential mechanism involving relevant proteins expression. The effects of Aspirin on human ovari- an cells were described from many different stages of cell growth, differentiation and migration. In addition, the po- tential mechanisms were demonstrated from cell apoptosis and proteins expression. Results The aspirin concentra- tions in treating cells HO8910 were range from 10, 5, 2 mmol · L^-1. VB2 was involved as controls with suggested concentration range from 40, 20, 10 mmol · L^-1. The cell surface markers percentages of CD44 ＋ and CD133 ＋ cells were decreased in both Aspirin and VB2 treated human ovarian cells （less than 80% ） , compared to that of control group （86%）. Cell proliferation viability, when the concentration of aspirin was 20 mmol · L^-1, the cell viability can be less than 40% of control and after 48 h and 72 h, the cell viability in Aspirin treated group contin- ued to be decreased, approaching 0%. For VB2, a concentration range wider than suggested concentration was in- volved in this detection （0. 25 - 100 μmol · L^-l）. After the first 24 h of VB2 treatment, the cell viability re- mained to be higher than 80 % of blank control. Colony formation of cells. The increased Aspirin concentration would lead to significantly decreased colony formation. Cell apoptosis, In aspirin treated groups, the ratio of cell ap- optosis （3%- 20% ） were greatly increased compared with control group （0.5%）, The expression of NK-KB and TNF-α,In Aspirin treated group, the expression level of TNF-α was increased with high concentration of aspirin, while the level of NK-KB was negative correlated with the level of TNF-α, which were down-regulated compared to control group. Conclusions In this study, the inhibitory effects of Aspirin on human ovarian cells HO8910 were demonstrated and analyzed. The cell viability, migration and colony formation would be suppressed after Aspirin treatment, without strict dose-effect. The cell apoptosis can be found after Aspirin treatment, as well as elevated ROS and reduced mitochondria membrane potential. The expression levels of relevant cytokines indicated down-reg- ulated NF-KB and up-regulated TNF-α, which may be the potential molecular mechanism for the tumor cell sup- pression effects of Aspirin. Cell migration with Transwell detection, The cells were treated with Aspirin and VB2 of different concentrations for 48 h （Table 2 and Figure 2 ）. Compared to the cell migration rate in control group （more than 80% ） , the cell migration was greatly reduced in both Aspirin and VB2 treated groups, which were both less than 40 %. Reactive oxygen, The reactive oxygen levels in Aspirin treated cells were greatly increased tobe 2-3 times compared to control group（Figure 3）. The dose-effect was not significant.
文摘Objective: The present study was designed to evaluate the effect a commonly prescribed Non Steroidal Anti Inflammatory Drug(NSAID) i.e.aspirin on brush border membrane in terms of changes in the intestinal transport level of glucose which is monosaccharide with absolute requirement in the body and hence its absorption is directly proportional on the morphology of the intestinal mucosa.Method: Albino rats(Rattus Norvegicus) were divided into two different groups,Group I(Control),Group II(aspirin-treated,50 mg aspirin/kg of body weight).The treatment was continued for 28 days.On the 29th day after overnight fasting,intestine was removed from animals of both groups.Changes in transport of glucose-D in intestine were studied.Result: The results indicated a significant decrease in the transport of glucose-D in aspirin treated group as compared to the control group.Conclusion: Cautious use of NSAID is recommended in commonly observed symptom such as headache and to those patients who are given as a prophylaxis for thrombosis.
文摘A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of aspirin (ASA) and its metabolite salicylic acid (SA) in human plasma has been developed and validated.The plasma ASA and SA were extracted using a liquid-liquid extraction (LLE) and the sample extract was injected onto the LC-MS/MS system.The limits of quantitation(LLOQ) for ASA and SA are both 25 ng/mL.This method allowed the reproducible and accurate quantification with the concentration rang of 25-10 000 ng/mL.This method could be applied to the quantitation aspirin and salicylic acid in human plasma.
文摘自发性脑出血是全球范围内致死致残的重要疾病之一,尽管其外科手术治疗已日趋成熟,但相应的术后管理方案尚不明晰。其中,脑出血术后存活患者发生主要心脑及外周血管事件的风险显著增加,但现行指南尚未明确能否以及何时启动抗血小板治疗。近期发表的自发性脑出血患者神经外科手术后早期抗血小板治疗(early-start antiplatelet treatment after neurosurgery in patients with spontaneous intracerebral hemorrhage,E-start)研究表明,对于具有高缺血事件风险的脑出血患者,术后早期启动抗血小板治疗可带来显著获益,且不增加出血风险。作为国内首个脑出血围手术期管理的高级别循证证据,E-start研究有助于提高脑出血救治与管理水平,改善患者预后。
文摘颅内动脉粥样硬化性狭窄（intracranial atherosclerotic stenosis,ICAS）是卒中的主要病因,也是卒中复发的高危因素。华法林-阿司匹林治疗症状性颅内动脉疾病试验（Warfarin and Aspirin for Symptomatic Intracranial Disease,WASID）研究对象为50%～99%颅内血管狭窄的患者,
