The control and prevention of pasteurellosis in rabbits which makes the hosts unsuitable for experimental use raised the needs to improve and simplify the procedures of Enzyme-Linked Immunosorbent Assay (ELISA) for de...The control and prevention of pasteurellosis in rabbits which makes the hosts unsuitable for experimental use raised the needs to improve and simplify the procedures of Enzyme-Linked Immunosorbent Assay (ELISA) for detect of antibody against P.multocida. A comparison on the sensitivity and specificity of bacterial culture of antemortem and postmortem samples, complement fixation test and enzyme-linked immunosorbent assay of 11 apparently healthy adult rabbits was conducted. The incidence rates showed 45.45%,54.54% and 72.73% respectively. The sensitivity for the three methods were 0.63, 0.67,and 1.00,and specificity for them were 1.00, 0.67 and 1.00 respectively. Somatic serotypes of isolates of P.multocida from rabbits of three groups (rabbits of group 2 were with clinic signs, those of groups 1 and 3 were apparently healthy) revealed no remarkable differences,and the predominant types were type 3 and type 3, 4. This was somewhat different from the reports derived from other states. As the antigen of different serotype used in ELISA may have different sensitivity and specificity, which is affected also by different preparation method, a type non-specific antigen should be selected to meet such request. The trial of accomplishment of ELISA without positive and negative controls was presented for discussion.展开更多
In the last ten-odd years, Chinese oceanographers have accomplished alarge amount of work in analysing the characteristics,distribution andvariation of temperature, salinity, density and water masses in shallow seaare...In the last ten-odd years, Chinese oceanographers have accomplished alarge amount of work in analysing the characteristics,distribution andvariation of temperature, salinity, density and water masses in shallow seaareas near China and in their forecasting methods, and have formally pub-lished agreat number of treatises. If these treatises, which are scattered展开更多
文摘The control and prevention of pasteurellosis in rabbits which makes the hosts unsuitable for experimental use raised the needs to improve and simplify the procedures of Enzyme-Linked Immunosorbent Assay (ELISA) for detect of antibody against P.multocida. A comparison on the sensitivity and specificity of bacterial culture of antemortem and postmortem samples, complement fixation test and enzyme-linked immunosorbent assay of 11 apparently healthy adult rabbits was conducted. The incidence rates showed 45.45%,54.54% and 72.73% respectively. The sensitivity for the three methods were 0.63, 0.67,and 1.00,and specificity for them were 1.00, 0.67 and 1.00 respectively. Somatic serotypes of isolates of P.multocida from rabbits of three groups (rabbits of group 2 were with clinic signs, those of groups 1 and 3 were apparently healthy) revealed no remarkable differences,and the predominant types were type 3 and type 3, 4. This was somewhat different from the reports derived from other states. As the antigen of different serotype used in ELISA may have different sensitivity and specificity, which is affected also by different preparation method, a type non-specific antigen should be selected to meet such request. The trial of accomplishment of ELISA without positive and negative controls was presented for discussion.
文摘为了给《2020年天文和天体物理学十年调查》中的中型任务概念即＂探测器概念研究＂（Probe Mission Concept Studies）提供输入,美国NASA征集了相关任务概念建议,即＂天体探测器任务概念研究＂（Astrophysics Probes Mission Concept Studies）。此次征集作为NASA＂空间和地球科学＂计划框架下的一个研究机会公告（Research Opportunities in Space and Earth Sciences,ROSES）,
文摘In the last ten-odd years, Chinese oceanographers have accomplished alarge amount of work in analysing the characteristics,distribution andvariation of temperature, salinity, density and water masses in shallow seaareas near China and in their forecasting methods, and have formally pub-lished agreat number of treatises. If these treatises, which are scattered
