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D-塔格糖3-差向异构酶的理性设计与机制解析
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作者 郭丁钰 魏婉清 +4 位作者 宋伟 吴静 闻建 胡贵鹏 刘立明 《食品与发酵工业》 北大核心 2025年第4期58-64,共7页
D-阿洛酮糖是一种在自然界中存在极少的酮糖,因其具有与蔗糖相近的甜度但热量更低而备受关注。然而,D-阿洛酮糖的高效生物合成仍然面临挑战,是由于用于生产的酮糖3-差向异构酶的热稳定性较差。该研究通过理性设计,引入二硫键和属性嫁接... D-阿洛酮糖是一种在自然界中存在极少的酮糖,因其具有与蔗糖相近的甜度但热量更低而备受关注。然而,D-阿洛酮糖的高效生物合成仍然面临挑战,是由于用于生产的酮糖3-差向异构酶的热稳定性较差。该研究通过理性设计,引入二硫键和属性嫁接策略,成功获得了热稳定性更高的最佳突变体M03。最佳突变体M03的T m值从52.7℃提高到69.4℃;在无需外源添加金属离子的条件下,55℃时半衰期延长至254.3 min,是野生型的54.1倍;通过分子动力学模拟和结构分析揭示了性能提升的详细机制。该研究为工业化生产D-阿洛酮糖提供了一个有潜力的突变菌株。 展开更多
关键词 d-阿洛酮糖 d-塔格糖3-差向异构酶 热稳定性 蛋白质工程改造 分子动力学模拟
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Numerical Simulation of the Protective Effect of Complex Boundaries Toward Shock Waves in a 3D Explosive Field 被引量:3
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作者 吴开腾 宁建国 《Journal of Beijing Institute of Technology》 EI CAS 2003年第1期50-54,共5页
A numerical method is presented that simulates 3D explosive field problems. A code MMIC3D using this method can be used to simulate the propagation and reflected effects of all kinds of rigid boundaries to shock waves... A numerical method is presented that simulates 3D explosive field problems. A code MMIC3D using this method can be used to simulate the propagation and reflected effects of all kinds of rigid boundaries to shock waves produced by an explosive source. These numerical results indicate that the code MMIC3D has the ability in computing cases such as 3D shock waves produced by air explosion, vortex region of the shock wave, the Mach wave, and reflected waves behind rigid boundaries. 展开更多
关键词 explosive field shock wave MMIC3d numerical simulation complex boundaries
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Study of 3-D Numerical Simulation for Gas Transfer in the Goaf of the Coal Mining 被引量:12
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作者 WU Zheng-yan JIANG Shu-guang HE Xin-jian WANG Lan-yun LIN Bai-quan 《Journal of China University of Mining and Technology》 EI 2007年第2期152-157,共6页
In order to simulate field distribution rules,mathematical models for 3-D air flows and gas transfer in the goaf of the coal mining are established,based on theories of permeability and dynamic dispersion through poro... In order to simulate field distribution rules,mathematical models for 3-D air flows and gas transfer in the goaf of the coal mining are established,based on theories of permeability and dynamic dispersion through porous media. A gas dispersion equation in a 3-D field is calculated by use of numerical method on a weighted upstream multi-element balance. Based on data of an example with a U type ventilation mode,surface charts of air pressure distribution and gas concentration are drawn by Graphtool software. Finally,a comparison between actually measured results in the model test and the numerical simulation results is made to proves the numerical implementation feasible. 展开更多
关键词 3d numerical simulation of gas transfer in the goaf air pressure distribution in the goaf weighted upstream multi-element balance numerical simulation method
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Numerical Simulation of 2D and 3D Sloshing Waves in a Regularly and Randomly Excited Container 被引量:2
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作者 Eswaran M Akashdeep S. Virk Ujjwal K. Saha 《Journal of Marine Science and Application》 2013年第3期298-314,共17页
In this paper,various aspects of the 2D and 3D nonlinear liquid sloshing problems in vertically excited containers have been studied numerically along with the help of a modified-transformation.Based on this new numer... In this paper,various aspects of the 2D and 3D nonlinear liquid sloshing problems in vertically excited containers have been studied numerically along with the help of a modified-transformation.Based on this new numerical algorithm,a numerical study on a regularly and randomly excited container in vertical direction was conducted utilizing four different cases: The first case was performed utilizing a 2D container with regular excitations.The next case examined a regularly excited 3D container with two different initial conditions for the liquid free surface,and finally,3D container with random excitation in the vertical direction.A grid independence study was performed along with a series of validation tests.An iteration error estimation method was used to stop the iterative solver(used for solving the discretized governing equations in the computational domain) upon reaching steady state of results at each time step.In the present case,this method was found to produce quite accurate results and to be more time efficient as compared to other conventional stopping procedures for iterative solvers.The results were validated with benchmark results.The wave elevation time history,phase plane diagram and surface plots represent the wave nonlinearity during its motion. 展开更多
关键词 3d container free surface σ-transformation sloshing wave finite difference method Numerical simulation
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Effect of Valence Band Tail Width on the Open Circuit Voltage of P3HT:PCBM Bulk Heterojunction Solar Cell:AMPS-1D Simulation Study 被引量:5
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作者 Bushra Mohamed Omer 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第8期216-220,共5页
The effect of the valence band tail width on the open circuit voltage of P3HT:PCBM bulk heterojunction solar cell is investigated by using the AMPS-1D computer program. An effective medium model with exponential vale... The effect of the valence band tail width on the open circuit voltage of P3HT:PCBM bulk heterojunction solar cell is investigated by using the AMPS-1D computer program. An effective medium model with exponential valence and conduction band tail states is used to simulate the photovoltaic cell. The simulation result shows that the open circuit voltage depends Iinearly on the logarithm of the generation rate and the slope depends on the width of the valence band tail. The open circuit voltage decreases with the increasing width of the band tail. The dark and light ideality factors increase with the width of the valence band tail. 展开更多
关键词 Effect of Valence Band Tail Width on the Open Circuit Voltage of P3HT:PCBM Bulk Heterojunction Solar Cell:AMPS-1d simulation Study HT
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Magnetic field of mathematical modeling and simulation of 3D magnetic pole array spherical actuator
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作者 孟宏君 王占林 焦中夏 《Journal of Beijing Institute of Technology》 EI CAS 2015年第1期97-104,共8页
In this paper a new spherical actuator is designed and its advantages are compared to an existing spherical actuator, which function is limited by several design bottlenecks. First the output torque is too small. Seco... In this paper a new spherical actuator is designed and its advantages are compared to an existing spherical actuator, which function is limited by several design bottlenecks. First the output torque is too small. Second, the attitude is difficult to be accurately detected. The new three-dimen- sional magnetic pole array can solve these major problems. The new actuator features an outer rotor with multiple permanent magnet (PM) poles. Using an analytical solution and the finite element so- lution simulation, the feasibility of the approach is verified. A prototype was developed, tested, and experiments were conducted to obtain the practical value of the magnetic flux density. 展开更多
关键词 3-d magnetic pole array spherical actuator simulation HALBACH motor design
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Numerical simulation of protection range in exploiting the upper protective layer with a bow pseudo-incline technique 被引量:15
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作者 HU Guo-zhong WANG Hong-tu LI Xiao-hong FAN Xiao-gang YUAN Zhi-gang 《Mining Science and Technology》 EI CAS 2009年第1期58-64,共7页
The developing processes of stress and deformation fields of a protected layer after mining an upper-protective layer with a bow pseudo-incline technique were simulated to locate the protection region. The pressure re... The developing processes of stress and deformation fields of a protected layer after mining an upper-protective layer with a bow pseudo-incline technique were simulated to locate the protection region. The pressure relief of the protected layer was analyzed after mining the upper-protective layer. The pressure relief angle along the strike and incline were located according to the roles of protection of the deformation and stress pressure-relief of the protective layer after mining. This results show that the upper-protective layer with the bow pseudo-incline technique have an upper and downside pressure relief angle of 85 and 68 degrees respectively; the distribution of strike pressure relief angles along the pseudo-incline working face is uneven and their values range from 38.3 to 51 degrees. The pressure relief angle of the inclined middle location was the largest. The distribution of the protection region of the upper-protective layer with the bow pseudo-incline teelmique located by practical tests and numerical simulation is essentially consistent, compared with the results obtained by these methods. 展开更多
关键词 bow pseudo-incline technique upper-protective layer protection region 3d numerical simulation
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Fast and Stable Surface Feature Simulation for Particle-Based Fluids 被引量:2
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作者 Xiaokun Wang Yanrui Xu +1 位作者 Xiaojuan Ban Pengfei Ye 《Journal of Beijing Institute of Technology》 EI CAS 2019年第1期57-66,共10页
In order to efficiently and realistically capture microscopic features of fluid surface,a fast and stable surface feature simulation approach for particle-based fluids is presented in this paper.This method employs a ... In order to efficiently and realistically capture microscopic features of fluid surface,a fast and stable surface feature simulation approach for particle-based fluids is presented in this paper.This method employs a steady tension and adhesion model to construct surface features with the consideration of the adsorption effect of fluid to solid.Molecular cohesion and surface area minimization are appended for surface tension,and adhesion is added to better show the microscopic characteristics of fluid surface.Besides,the model is integrated to an implicit incompressible smoothed particle hydrodynamics(SPH)method to improve the efficiency and stability of simulation.The experimental results demonstrate that the method can better simulates surface features in a variety of scenarios stably and efficiently. 展开更多
关键词 virtual REALITY 3d visualization FLUId simulation surface FEATURE
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Three-dimensional simulation of fabrication process-dependent effects on single event effects of SiGe heterojunction bipolar transistor 被引量:2
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作者 张晋新 贺朝会 +3 位作者 郭红霞 李培 郭宝龙 吴宪祥 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第8期542-550,共9页
The fabrication process dependent effects on single event effects (SEEs) are investigated in a commercial silicon- germanium heterojunction bipolar transistor (SiGe HBT) using three-dimensional (3D) TCAD simulat... The fabrication process dependent effects on single event effects (SEEs) are investigated in a commercial silicon- germanium heterojunction bipolar transistor (SiGe HBT) using three-dimensional (3D) TCAD simulations. The influences of device structure and doping concentration on SEEs are discussed via analysis of current transient and charge collection induced by ions strike. The results show that the SEEs representation of current transient is different from representation of the charge collection for the same process parameters. To be specific, the area of C/S junction is the key parameter that affects charge collection of SEE. Both current transient and charge collection are dependent on the doping of collector and substrate. The base doping slightly influences transient currents of base, emitter, and collector terminals. However, the SEEs of SiGe HBT are hardly affected by the doping of epitaxial base and the content of Ge. 展开更多
关键词 SiGe HBT single event effects fabrication process dependence 3d simulation
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Simulation of Polymer Melt Flow Fields in Intermeshing Co-Rotating Three-Screw Extruders 被引量:5
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作者 胡冬冬 陈晋南 《Journal of Beijing Institute of Technology》 EI CAS 2006年第3期360-365,共6页
Three-dimension isothermal flows of polymer melt in the kneading blocks of triangularly-arranged and parallelly-arranged intermeshing co-rotating three-screw extruders are simulated using the finite element package PO... Three-dimension isothermal flows of polymer melt in the kneading blocks of triangularly-arranged and parallelly-arranged intermeshing co-rotating three-screw extruders are simulated using the finite element package POLYFLOW. Based on the velocity fields calculated, the particle trajectories in both machines are visualized using particle tracking technique. The numerical results indicate that the flow patterns in three-screw extruders are similar to those in twin-screw extruders. The triangularly-arranged three-screw extruder has the largest pumping capacity and also the highest extrusion stability in terms of flowrate fluctuation with screw rotation. The instantaneous mixing and cumulative residence time distribution (RTD) characteristics are also analyzed and compared with traditional intermeshing co-rotating twin-screw extruders. It is shown that the start section of the cumulative RTD curve for the triangularly-arranged machine has a small shoulder, which is attributed to the faster flow in the central region of this type of extruder. 展开更多
关键词 numerical simulation 3-d isothermal flow three-screw extruders
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Numerical simulation of the flow field of a flat torque converter 被引量:6
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作者 闫清东 刘城 魏巍 《Journal of Beijing Institute of Technology》 EI CAS 2012年第3期309-314,共6页
A flexible flat torque converter was proposed to fulfill the requirement of miniaturization and power density maximization for automobiles.Constructed by two arcs joined by lines,the torus was designed directly from d... A flexible flat torque converter was proposed to fulfill the requirement of miniaturization and power density maximization for automobiles.Constructed by two arcs joined by lines,the torus was designed directly from design path.The influence of flatness on the performance of the torque converter was evaluated.The software CFX and standard k-ε model were adopted to simulate the internal flow fields of the torque converter under different flatness ratios.The results indicated that the performance of the torque converter got worse as the flatness declined,but the capacity of pump increased.The efficiency and the torque ratio dropped slightly as the flatness ratio decreased.So the torque converter could be squashed appropriately to get high power density without too much efficiency sacrifice.But when the flatness ratio was below 0.2,there was a significant drop in the efficiency. 展开更多
关键词 torque converter 3d flow simulation flatness ratio efficiency high power density
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Fully Nonlinear Simulation for Fluid/Structure Impact:A Review 被引量:2
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作者 Shili Sun Guoxiong Wu 《Journal of Marine Science and Application》 2014年第3期237-244,共8页
This paper presents a review of the work on fluid/structure impact based on inviscid and imcompressible liquid and irrotational flow. The focus is on the velocity potential theory together with boundary element method... This paper presents a review of the work on fluid/structure impact based on inviscid and imcompressible liquid and irrotational flow. The focus is on the velocity potential theory together with boundary element method (BEM). Fully nonlinear boundary conditions are imposed on the unknown free surface and the wetted surface of the moving body. The review includes (1) vertical and oblique water entry of a body at constant or a prescribed varying speed, as well as free fall motion, (2) liquid droplets or column impact as well as wave impact on a body, (3) similarity solution of an expanding body. It covers two dimensional (2D), axisymmetric and three dimensional (3D) cases. Key techniques used in the numerical simulation are outlined, including mesh generation on the multivalued free surface, the stretched coordinate system for expanding domain, the auxiliary function method for decoupling the mutual dependence of the pressure and the body motion, and treatment for the jet or the thin liquid film developed during impact. 展开更多
关键词 fluid/structure impact boundary element method 3d surface mesh generation water entry wave impact similarity solution fully nonlinear simulation
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Parallel numerical simulations for quantized vortices in Bose-Einstein condensates
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作者 黄朝晖 王德生 《Chinese Physics B》 SCIE EI CAS CSCD 2007年第1期32-37,共6页
We employ the parallel computing technology to study numerically the three-dimensional structure of quantized vortices of Bose-Einstein condensates, For anisotropic cases, the bending process of vortices is described ... We employ the parallel computing technology to study numerically the three-dimensional structure of quantized vortices of Bose-Einstein condensates, For anisotropic cases, the bending process of vortices is described in detail by the decrease of Gross-Pitaevskii energy. A completely straight vortex and the steady and symmetrical multiple-vortex configurations are obtained. We analyse the effect of initial conditions and angular velocity on the number and shape of vortices. 展开更多
关键词 3d numerical simulations quantized vortices Bose-Einstein condensates parallel computing
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Three-dimensional Monte Carlo simulation of bulk fin field effect transistor
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作者 王骏成 杜刚 +2 位作者 魏康亮 张兴 刘晓彦 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第11期421-426,共6页
In this paper, we investigate the performance of the bulk fin field effect transistor (FinFET) through a three- dimensional (3D) full band Monte Carlo simulator with quantum correction. Several scattering mechanis... In this paper, we investigate the performance of the bulk fin field effect transistor (FinFET) through a three- dimensional (3D) full band Monte Carlo simulator with quantum correction. Several scattering mechanisms, such as the acoustic and optical phonon scattering, the ionized impurity scattering, the impact ionization scattering and the surface roughness scattering are considered in our simulator. The effects of the substrate bias and the surface roughness scattering near the Si/SiO2 interface on the performance of bulk FinFET are mainly discussed in our work. Our results show that the on-current of bulk FinFET is sensitive to the surface roughness and that we can reduce the substrate leakage current by modulating the substrate bias voltage. 展开更多
关键词 bulk fin field effect transistor (FinFET) three-dimensional 3d Monte Carlo simulation surface roughness scattering substrate bias effect
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基于RNG k-ε湍流模型的3D采空区瓦斯上浮贮移 被引量:40
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作者 李宗翔 顾润红 +2 位作者 张晓明 毕强 温永宇 《煤炭学报》 EI CAS CSCD 北大核心 2014年第5期880-885,共6页
为研究工作面后方采空区3D空间瓦斯上浮特征,根据质量守恒、动量守恒和Fick定律,建立综放采空区风流-瓦斯变密度混合气体非线性渗流-扩散控制方程。结合大兴矿N2-706工作面实例,运用CFD软件模拟三维采空区瓦斯运移及分布的状态;流场冒... 为研究工作面后方采空区3D空间瓦斯上浮特征,根据质量守恒、动量守恒和Fick定律,建立综放采空区风流-瓦斯变密度混合气体非线性渗流-扩散控制方程。结合大兴矿N2-706工作面实例,运用CFD软件模拟三维采空区瓦斯运移及分布的状态;流场冒落非均质按照"O"型分布描述,计算采用RNGk-ε湍流模型,并考虑重力场条件。理论计算调整与现场实际条件和瓦斯监测记录数据相拟合,指出三维采空区形成的瓦斯分布及上浮态势,是风流移动、瓦斯组分扩散-弥散和含瓦斯风流密度差引起上浮迁移的结果,也是流场瓦斯不断解吸涌出与漏风不断流入所形成的一种动态平衡结果。研究结果表明,采空区高位抽采流量与抽采获得的瓦斯体积分数近似呈反比例函数关系、与回风巷瓦斯体积分数呈负指数函数关系。 展开更多
关键词 3d采空区 瓦斯上浮 CFd仿真 k-ε湍流模型 瓦斯抽采
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基于3D扫描的马铃薯建模及运动仿真分析 被引量:8
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作者 蒙建国 王春光 +2 位作者 谢胜仕 宿金殿 祁少华 《农机化研究》 北大核心 2015年第10期64-67,共4页
以马铃薯块茎形状不规则为前提,提出了一种新的马铃薯建模方法—3D扫描数据采集法,基于3D扫描数据采集软件中的采集功能,实现对不规则马铃薯的三维建模。随机选择两种形状不同、质量相近的马铃薯块茎,通过3D扫描技术建立虚拟网格模型,... 以马铃薯块茎形状不规则为前提,提出了一种新的马铃薯建模方法—3D扫描数据采集法,基于3D扫描数据采集软件中的采集功能,实现对不规则马铃薯的三维建模。随机选择两种形状不同、质量相近的马铃薯块茎,通过3D扫描技术建立虚拟网格模型,并导入离散元分析软件,建立其虚拟模型,设置摆动筛的筛面倾角5.8°、振幅3 5 mm、振动频率5.5 Hz,基于离散元素法对摆动筛的运动进行仿真分析,分析马铃薯在筛面上的运动轨迹及受力变化,为研究马铃薯块茎在筛面上的运动规律提供理论依据和方法。 展开更多
关键词 马铃薯 建模 仿真 3d扫描 离散元
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考虑平稳风修正和塔架干扰的风力机叶片3-D风场模拟 被引量:2
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作者 柯世堂 曹九发 +1 位作者 王珑 王同光 《南京航空航天大学学报》 EI CAS CSCD 北大核心 2015年第1期153-159,共7页
为准确模拟风力机纵向、横向和垂直向的3-D随机风场,以南京航空航天大学自主研发的NH1500风力机为例,首先采用考虑沿高度变化的指数模型、塔影效应影响的潜流模型和上游风机尾流影响的涡流粘度模型对平稳风进行修正。然后基于空间变化... 为准确模拟风力机纵向、横向和垂直向的3-D随机风场,以南京航空航天大学自主研发的NH1500风力机为例,首先采用考虑沿高度变化的指数模型、塔影效应影响的潜流模型和上游风机尾流影响的涡流粘度模型对平稳风进行修正。然后基于空间变化的改进Von Karman风谱模型,结合谐波叠加法模拟风力机的来流脉动风速时程,再利用改进的叶素-动量理论获得考虑叶片旋转效应和塔架-叶片相干效应的风力机纵向、横向和垂直向3-D随机风场。该方法充分考虑风力机风场模拟的外界干扰因素和自身特性,提高了风力机系统风场模拟的准确度。算例分析表明,本文方法可以准确地模拟给定风环境的风力机系统3-D风场。 展开更多
关键词 风力机 3-d风场模拟 平稳风修正 塔架干扰 改进的叶素-动量理论
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基于GIS和3D技术的西江航道信息系统 被引量:2
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作者 刘俊涛 邓明文 +2 位作者 姜冲 李杰 纪红军 《水利科技与经济》 2012年第7期102-104,共3页
本系统以西江航道的长洲段为研究对象,以基础地形图和各类航道相关的数据要素为平台,以航道相关地理信息的空间数据和属性数据为核心,利用计算机技术、地理信息系统(GIS)技术、数据库技术、三维虚拟仿真技术,建立起一个能快速提供真实... 本系统以西江航道的长洲段为研究对象,以基础地形图和各类航道相关的数据要素为平台,以航道相关地理信息的空间数据和属性数据为核心,利用计算机技术、地理信息系统(GIS)技术、数据库技术、三维虚拟仿真技术,建立起一个能快速提供真实准确的地理信息、便于查询统计及决策分析的信息系统;同时能对CAD数据、GIS数据、遥感影像等数据进行快速的读入,将制作的专题图导出。为航道的数据查询、三维模拟显示、专题分析与制作提供强大的解决方案。 展开更多
关键词 数据查询 三维模拟 专题制图 西江航道
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软件工程化方法在武器3D实体建模中的应用研究 被引量:1
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作者 赵文婷 康凤举 +1 位作者 魏佳宁 宋强 《鱼雷技术》 2008年第1期56-59,共4页
针对复杂武器3D实体建模的需求,分析并比较了软件工程化中的瀑布模型、变换模型和增量模型等3种模型开发方法,首次将增量模型方法引入到武器3D实体建模中。基本思路是,首先根据需求分析得到的信息将武器3D实体模型划分为不同的增量构件... 针对复杂武器3D实体建模的需求,分析并比较了软件工程化中的瀑布模型、变换模型和增量模型等3种模型开发方法,首次将增量模型方法引入到武器3D实体建模中。基本思路是,首先根据需求分析得到的信息将武器3D实体模型划分为不同的增量构件,然后对增量构件进行详细设计并完成基于MultiGen Creator的构件开发与测试,最后进行增量构件组装和模型测试。结果表明,应用该方法有利于提高模型的可重用性和开发效率,同时使开发的模型满足视景仿真系统的逼真性和实时性的要求。 展开更多
关键词 3d实体建模 软件工程化 增量模型 瀑布模型 变换模型 视景仿真系统
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基于蒙特卡罗模拟3-D PET图像重建的研究
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作者 林桂粉 罗立民 《原子能科学技术》 EI CAS CSCD 北大核心 2008年第12期1143-1148,共6页
应用蒙特卡罗模拟软件GATE对Ge Discovery LS PET/CT扫描系统进行模拟,获得三维投影数据,分别采用三维有序子集最大期望值法(3-D OSEM)与三维滤波反投影法(3-D FBP)进行图像重建,从空间分辨率、成像的对比度以及信噪比等方面对3-D OSEM... 应用蒙特卡罗模拟软件GATE对Ge Discovery LS PET/CT扫描系统进行模拟,获得三维投影数据,分别采用三维有序子集最大期望值法(3-D OSEM)与三维滤波反投影法(3-D FBP)进行图像重建,从空间分辨率、成像的对比度以及信噪比等方面对3-D OSEM法与3-D FBP法的重建结果进行比较。结果显示,对蒙特卡罗方法模拟获得的三维投影数据,采用3-D OSEM法重建比3-D FBP法重建得到的图像质量好。 展开更多
关键词 蒙特卡罗模拟 GATE软件 图像重建 3-d OSEM 3-d FBP
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