Increased circulating branched-chain amino acids(BCAAs)have been involved in the pathogenesis of obesity and insulin resistance.However,evidence relating berberine(BBR),gut microbiota,BCAAs,and insulin resis⁃tance is ...Increased circulating branched-chain amino acids(BCAAs)have been involved in the pathogenesis of obesity and insulin resistance.However,evidence relating berberine(BBR),gut microbiota,BCAAs,and insulin resis⁃tance is limited.Here,we showed that BBR could effectively rectify steatohepatitis and glucose intolerance in high-fat diet(HFD)-fed mice.BBR reorganized gut microbiota populations under both the normal chow diet(NCD)and HFD.Particu⁃larly,BBR noticeably decreased the relative abundance of BCAA-producing bacteria,including order Clostridiales;fami⁃lies Streptococcaceae,Clostridiaceae,and Prevotellaceae;and genera Streptococcus and Prevotella.Compared with the HFD group,predictive metagenomics indicated a reduction in the proportion of gut microbiota genes involved in BCAA biosynthesis but the enrichment genes for BCAA degradation and transport by BBR treatment.Accordingly,the elevated serum BCAAs of HFD group were significantly decreased by BBR.Furthermore,the Western blotting results implied that BBR could promote the BCAA catabolism in the liver and epididymal white adipose tissues of HFD-fed mice by acti⁃vation of the multienzyme branched-chain α-ketoacid dehydrogenase complex,whereas by inhibition of the phosphoryla⁃tion state of BCKDHA(E1α subunit)and branched-chain α-ketoacid dehydrogenase kinase.The ex vivo assay further confirmed that BBR could increase BCAA catabolism in both AML12 hepatocytes and 3T3-L1 adipocytes.Finally,data from healthy subjects and diabetics confirmed that BBR could improve glycemic control and modulate circulating BCAAs.Besides,functional microbiomics integrated high-throughput microbial genomics,metabolomics and molecular biotechnology has also been successfully applied to reveal the anti-obesity mechanism of hydroxysafflor yellow A.展开更多
Different from the organization structure of complex projects in Western countries, the Liang Zong hierarchical organization structure of complex projects in China has two different chains, the chief-engineer chain an...Different from the organization structure of complex projects in Western countries, the Liang Zong hierarchical organization structure of complex projects in China has two different chains, the chief-engineer chain and the general-director chain,to handle the trade-off between technical and management decisions. However, previous works on organization search have mainly focused on the single-chain hierarchical organization in which all decisions are regarded as homogeneous. The heterogeneity and the interdependency between technical decisions and management decisions have been neglected. A two-chain hierarchical organization structure mapped from a real complex project is constructed. Then, a discrete decision model for a Liang Zong two-chain hierarchical organization in an NK model framework is proposed. This model proves that this kind of organization structure can reduce the search space by a large amount and that the search process should reach a final stable state more quickly. For a more complicated decision mechanism, a multi-agent simulation based on the above NK model is used to explore the effect of the two-chain organization structure on the speed, stability, and performance of the search process. The results provide three insights into how, compared with the single-chain hierarchical organization, the two-chain organization can improve the search process: it can reduce the number of iterations efficiently; the search is more stable because the search space is a smoother hill-like fitness landscape; in general, the search performance can be improved.However, when the organization structure is very complicated, the performance of a two-chain organization is inferior to that of a single-chain organization. These findings about the efficiency of the unique Chinese-style organization structure can be used to guide organization design for complex projects.展开更多
E-supply chain is the trend of the supply chain ma na gement. However, building E-supply chain depends on business process improvemen t and re-engineering, which becomes one of the important aspects in supply chai n m...E-supply chain is the trend of the supply chain ma na gement. However, building E-supply chain depends on business process improvemen t and re-engineering, which becomes one of the important aspects in supply chai n management. However, the supply chain process re-engineering and improvement is constrained because of lack of integrating operation framework and standards based on information management. We find that the idea of CALS origins from DoD of USA can solve the problems very well. CALS is the abbreviation of "continues acquisition and life- cycle support". It is an information strategy adopted by many developed counties. The final objective of the CALS is that government a nd industry can generate, exchange, manage and use all the digital information t hroughout the lifecycle of a product in virtue of adopting the international and national standards, improving existing business process and introducing advance d technology. The Integrated Digital Environment (IDE) created by CALS provides a brand new operation mode for consumer, contractor and supplier in supply chain . Besides, a series mutual standard is involved in CALS system. These standards and the standardization technology would be applied widely in the supply chain. This study mainly focuses on supply chain process in CALS environment, and puts forward a framework model of supply chain process. Then the study analyzes the c haracteristics of the model, which includes the application of standards and inf ormation technology, and finally discusses on methods to achieve the supply chai n process in CALS environment.展开更多
文摘Increased circulating branched-chain amino acids(BCAAs)have been involved in the pathogenesis of obesity and insulin resistance.However,evidence relating berberine(BBR),gut microbiota,BCAAs,and insulin resis⁃tance is limited.Here,we showed that BBR could effectively rectify steatohepatitis and glucose intolerance in high-fat diet(HFD)-fed mice.BBR reorganized gut microbiota populations under both the normal chow diet(NCD)and HFD.Particu⁃larly,BBR noticeably decreased the relative abundance of BCAA-producing bacteria,including order Clostridiales;fami⁃lies Streptococcaceae,Clostridiaceae,and Prevotellaceae;and genera Streptococcus and Prevotella.Compared with the HFD group,predictive metagenomics indicated a reduction in the proportion of gut microbiota genes involved in BCAA biosynthesis but the enrichment genes for BCAA degradation and transport by BBR treatment.Accordingly,the elevated serum BCAAs of HFD group were significantly decreased by BBR.Furthermore,the Western blotting results implied that BBR could promote the BCAA catabolism in the liver and epididymal white adipose tissues of HFD-fed mice by acti⁃vation of the multienzyme branched-chain α-ketoacid dehydrogenase complex,whereas by inhibition of the phosphoryla⁃tion state of BCKDHA(E1α subunit)and branched-chain α-ketoacid dehydrogenase kinase.The ex vivo assay further confirmed that BBR could increase BCAA catabolism in both AML12 hepatocytes and 3T3-L1 adipocytes.Finally,data from healthy subjects and diabetics confirmed that BBR could improve glycemic control and modulate circulating BCAAs.Besides,functional microbiomics integrated high-throughput microbial genomics,metabolomics and molecular biotechnology has also been successfully applied to reveal the anti-obesity mechanism of hydroxysafflor yellow A.
基金supported by the National Natural Science Foundation of China(7157105771390522)the Key Lab for Public Engineering Audit of Jiangsu Province,Nanjing Audit University(GGSS2016-08)
文摘Different from the organization structure of complex projects in Western countries, the Liang Zong hierarchical organization structure of complex projects in China has two different chains, the chief-engineer chain and the general-director chain,to handle the trade-off between technical and management decisions. However, previous works on organization search have mainly focused on the single-chain hierarchical organization in which all decisions are regarded as homogeneous. The heterogeneity and the interdependency between technical decisions and management decisions have been neglected. A two-chain hierarchical organization structure mapped from a real complex project is constructed. Then, a discrete decision model for a Liang Zong two-chain hierarchical organization in an NK model framework is proposed. This model proves that this kind of organization structure can reduce the search space by a large amount and that the search process should reach a final stable state more quickly. For a more complicated decision mechanism, a multi-agent simulation based on the above NK model is used to explore the effect of the two-chain organization structure on the speed, stability, and performance of the search process. The results provide three insights into how, compared with the single-chain hierarchical organization, the two-chain organization can improve the search process: it can reduce the number of iterations efficiently; the search is more stable because the search space is a smoother hill-like fitness landscape; in general, the search performance can be improved.However, when the organization structure is very complicated, the performance of a two-chain organization is inferior to that of a single-chain organization. These findings about the efficiency of the unique Chinese-style organization structure can be used to guide organization design for complex projects.
文摘E-supply chain is the trend of the supply chain ma na gement. However, building E-supply chain depends on business process improvemen t and re-engineering, which becomes one of the important aspects in supply chai n management. However, the supply chain process re-engineering and improvement is constrained because of lack of integrating operation framework and standards based on information management. We find that the idea of CALS origins from DoD of USA can solve the problems very well. CALS is the abbreviation of "continues acquisition and life- cycle support". It is an information strategy adopted by many developed counties. The final objective of the CALS is that government a nd industry can generate, exchange, manage and use all the digital information t hroughout the lifecycle of a product in virtue of adopting the international and national standards, improving existing business process and introducing advance d technology. The Integrated Digital Environment (IDE) created by CALS provides a brand new operation mode for consumer, contractor and supplier in supply chain . Besides, a series mutual standard is involved in CALS system. These standards and the standardization technology would be applied widely in the supply chain. This study mainly focuses on supply chain process in CALS environment, and puts forward a framework model of supply chain process. Then the study analyzes the c haracteristics of the model, which includes the application of standards and inf ormation technology, and finally discusses on methods to achieve the supply chai n process in CALS environment.
