直链淀粉含量的快速高通量测定方法对国际标准法(简称"国际法",BS EN ISO6647-2:2015《稻米直链淀粉含量的测定第2部分常规方法》)进行了改良,可以极大地提高水稻育种材料的筛选效率、适应当前水稻稻米品质育种的新需求。结...直链淀粉含量的快速高通量测定方法对国际标准法(简称"国际法",BS EN ISO6647-2:2015《稻米直链淀粉含量的测定第2部分常规方法》)进行了改良,可以极大地提高水稻育种材料的筛选效率、适应当前水稻稻米品质育种的新需求。结果表明,高通量法测得的吸光度与直链淀粉含量有良好的相关性,相关系数达0.9911,该方法直链淀粉含量的测定值与国际法的测定值无显著差异;同时,高通量法还具有简单、易操作、样品量少、批量(96个样本)测定等优点,能用于水稻诱变群体后代或品种选育过程中的海量株系大规模初筛,可极大地提高稻米品质研究的效率。展开更多
The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many o...The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.展开更多
文摘直链淀粉含量的快速高通量测定方法对国际标准法(简称"国际法",BS EN ISO6647-2:2015《稻米直链淀粉含量的测定第2部分常规方法》)进行了改良,可以极大地提高水稻育种材料的筛选效率、适应当前水稻稻米品质育种的新需求。结果表明,高通量法测得的吸光度与直链淀粉含量有良好的相关性,相关系数达0.9911,该方法直链淀粉含量的测定值与国际法的测定值无显著差异;同时,高通量法还具有简单、易操作、样品量少、批量(96个样本)测定等优点,能用于水稻诱变群体后代或品种选育过程中的海量株系大规模初筛,可极大地提高稻米品质研究的效率。
文摘The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.
