Time-of-flight mass spectrometry (TOF-MS) is widely used in protein chemistry and proteomics research due to its high sensitivity , resolution and accuracy. Recently we have developed two Q-TOF-MS analytical systems t...Time-of-flight mass spectrometry (TOF-MS) is widely used in protein chemistry and proteomics research due to its high sensitivity , resolution and accuracy. Recently we have developed two Q-TOF-MS analytical systems titled as “ESI-TOF-MS detector of HPLC”, which have promising features and arrive at the coordinative level compared with the congener overseas production. The ESI-Orthogonal Acceleration-Reflecting-TOF mass spectrometry can detect the lysozyme sample concentration below 1 pmol/μL under the optimized conditions. The mass range of these instruments is from 50 to 6000dahon with a general ESI interface to the HPLC. The optimal mass resolution exceeds 6000(FWHM)and the signal/noise ratio of Reserpine (10 ng/L) is greater than 100.展开更多
采用高效液相色谱-光化学柱后在线衍生-荧光检测法检测鸡肉中16种磺胺类药物残留量。鸡肉样品经过乙腈提取并脱脂净化,色谱柱分离,光化学在线衍生并直接用荧光检测器检测。流动相为0.3%的冰乙酸和甲醇溶液梯度洗脱,流速0.7 m L/min、柱...采用高效液相色谱-光化学柱后在线衍生-荧光检测法检测鸡肉中16种磺胺类药物残留量。鸡肉样品经过乙腈提取并脱脂净化,色谱柱分离,光化学在线衍生并直接用荧光检测器检测。流动相为0.3%的冰乙酸和甲醇溶液梯度洗脱,流速0.7 m L/min、柱温36℃,激发波长320 nm、发射波长450 nm,16种磺胺类药物得到良好分离。16种磺胺药物在0.13~67.89μg/m L质量度范围内,线性良好（R≥0.994 0）,回收率在61.2%~106.6%之间,相对标准偏差为0.3%~13.9%,检出限为1.0~67.4μg/kg。该方法灵敏、准确、快速,可满足鸡肉中磺胺类残留的检测。展开更多
文摘Time-of-flight mass spectrometry (TOF-MS) is widely used in protein chemistry and proteomics research due to its high sensitivity , resolution and accuracy. Recently we have developed two Q-TOF-MS analytical systems titled as “ESI-TOF-MS detector of HPLC”, which have promising features and arrive at the coordinative level compared with the congener overseas production. The ESI-Orthogonal Acceleration-Reflecting-TOF mass spectrometry can detect the lysozyme sample concentration below 1 pmol/μL under the optimized conditions. The mass range of these instruments is from 50 to 6000dahon with a general ESI interface to the HPLC. The optimal mass resolution exceeds 6000(FWHM)and the signal/noise ratio of Reserpine (10 ng/L) is greater than 100.
文摘建立了高效液相色谱-荧光检测(HPLC-FLD)测定橄榄油中苯并[a]蒽、屈艹、苯并[b]荧蒽、苯并[a]芘4种多环芳烃(PAHs)的分析方法。橄榄油样品经异丙醇稀释,采用具有π-π特异性作用的固相萃取柱净化,Agilent ZORBAX Eclipse PAH色谱柱(100 m m×2.1 m m,1.8μm)分离,以水-乙腈为流动相,梯度洗脱,实现了4种化合物的基线分离,并用基质匹配校准溶液进行外标法定量。4种多环芳烃的线性范围为2.4~40μg/L,相关系数(r)为0.999 0~0.999 9,方法的定量限为0.147~0.413μg/L,加标回收率为95.5%~103.2%,日内和日间精密度(RSD)分别为0.10%~1.69%和2.48%~2.93%(n=5)。该法具有灵敏度高、检出限低、重复性好等特点,适用于橄榄油中4种PAHs快速、准确的定量检测。
