目的:评估以胶原、羟基磷灰石、硫酸软骨素等3种天然骨骼基本成分构建成的三维多孔骨修复材料的理化性能和体内生物学性能,观察其作为骨形态发生蛋白(bone morphogenetic protein 2,BMP-2)载体的效果。方法:以胶原、羟基磷灰石、硫酸软...目的:评估以胶原、羟基磷灰石、硫酸软骨素等3种天然骨骼基本成分构建成的三维多孔骨修复材料的理化性能和体内生物学性能,观察其作为骨形态发生蛋白(bone morphogenetic protein 2,BMP-2)载体的效果。方法:以胶原、羟基磷灰石、硫酸软骨素及BMP-2为原料,通过化学交联和冷冻干燥的方法构建具有一定三维结构的骨修复材料。通过HE染色、扫描电镜观察材料的结构性能;通过表面能谱、X线衍射观察材料的理化性能;将骨髓基质干细胞(marrow stromal cells,MSCs)种植在材料表面,观察MSCs在材料表面的粘附、增生和分化;将该复合材料种植在大鼠体内,观察材料在体内的降解和异位成骨情况。结果:骨修复材料在植入局部保持完整的支架结构,具有利于细胞粘附和增殖的多孔结构。通过肌肉埋植,在异位诱导形成了骨组织,并且随着骨组织的形成,支架逐渐降解吸收。结论:胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白是具有良好的生物相容性和骨诱导特性的骨修复材料。展开更多
Bone morphogenetic protein-1(BMP-1) and its related molecules are members of metalloendoproteinase astacin family, including BMP-1, mTLD, mTLL-1 and mTLL-2. Even though all of them lack of the ability to induce bone o...Bone morphogenetic protein-1(BMP-1) and its related molecules are members of metalloendoproteinase astacin family, including BMP-1, mTLD, mTLL-1 and mTLL-2. Even though all of them lack of the ability to induce bone or cartilage formation directly, they play key roles in numerable activities in ECM from embryo to adult, then affect the procedure and the result of osteogenesis and bone remodeling directly or indirectly. They are critical in maturation and deposition of some major collagen types, and in regulating the signaling of some growth factors in TGF-β superfamily by degradation of TGF-β inhibitor such as Chordin. The investigations about tissue distribution of BMP-1 and its related proteinases and also gene knock-out studies strongly indicate that they play key roles in osteogenesis and bone development.展开更多
Ti-based biomaterial has been widely used as dental and bone implant material although its bioacti-vity still needs improvement especially for dental implant.The bone morphogenetic proteins(BMPs) bound to Ti-based mat...Ti-based biomaterial has been widely used as dental and bone implant material although its bioacti-vity still needs improvement especially for dental implant.The bone morphogenetic proteins(BMPs) bound to Ti-based materials will attract the mesenchymal stem cells to differentiate into osteoblast cells,which benefits the response to the protein-material surface,and finally leads to new bone formation.Several methods including physically mixing,coating,plasma immobilization and cross-linking were used to investigate how BMPs bind to Ti-based biomaterials.The latest research papers are focused on the structure and function of BMPs,and the methods to bind BMPs to Ti-based biomaterial and the evaluation methods after protein immobilization are reviewed in this paper.展开更多
文摘Bone morphogenetic protein-1(BMP-1) and its related molecules are members of metalloendoproteinase astacin family, including BMP-1, mTLD, mTLL-1 and mTLL-2. Even though all of them lack of the ability to induce bone or cartilage formation directly, they play key roles in numerable activities in ECM from embryo to adult, then affect the procedure and the result of osteogenesis and bone remodeling directly or indirectly. They are critical in maturation and deposition of some major collagen types, and in regulating the signaling of some growth factors in TGF-β superfamily by degradation of TGF-β inhibitor such as Chordin. The investigations about tissue distribution of BMP-1 and its related proteinases and also gene knock-out studies strongly indicate that they play key roles in osteogenesis and bone development.
文摘Ti-based biomaterial has been widely used as dental and bone implant material although its bioacti-vity still needs improvement especially for dental implant.The bone morphogenetic proteins(BMPs) bound to Ti-based materials will attract the mesenchymal stem cells to differentiate into osteoblast cells,which benefits the response to the protein-material surface,and finally leads to new bone formation.Several methods including physically mixing,coating,plasma immobilization and cross-linking were used to investigate how BMPs bind to Ti-based biomaterials.The latest research papers are focused on the structure and function of BMPs,and the methods to bind BMPs to Ti-based biomaterial and the evaluation methods after protein immobilization are reviewed in this paper.
