目的探讨甲基-CpG结合蛋白1(methyl-CpG-binding domain protein 1,MBD1)缺失小鼠缓解弗氏完全佐剂(CFA)诱导的炎性疼痛是否通过抑制神经传导通路中的降钙素基因相关肽(CGRP)来实现的,为MBD1调控炎性疼痛的神经生物学机制提供新的思路...目的探讨甲基-CpG结合蛋白1(methyl-CpG-binding domain protein 1,MBD1)缺失小鼠缓解弗氏完全佐剂(CFA)诱导的炎性疼痛是否通过抑制神经传导通路中的降钙素基因相关肽(CGRP)来实现的,为MBD1调控炎性疼痛的神经生物学机制提供新的思路。方法(1)取8周龄雄性MBD1敲除小鼠(KO)和与KO小鼠年龄、性别和体质量配对C57BL6小鼠(WT),按随机数字表法分为4组,分别为做足底注射生理盐水(NS)、CFA的WT小鼠组和注射NS、CFA的KO小鼠组(每组7只)。观察药物前1天(-1 d)、注射后2 h、1、3、7和14 d 4组小鼠机械缩足反应频率(PWF)和热缩足反应潜伏期(PWL)行为改变;(2)另取CFA注射后1 d(疼痛最明显点)上述组小鼠(每组3只),分别提取患侧背根神经节(DRG)和脊髓背角组织行ELISA法检测CGRP含量,比较4组含量变化。结果(1)与WT小鼠比较,KO小鼠CFA注射前明显降低双侧后爪基础PWF和延长PWL;与注射生理盐水的WT小鼠组比较,CFA注射后2 h、1、3、7和14 d WT小鼠患侧PWF增加(P<0.05)、PWL降低(P<0.05),而注射CFA的KO小鼠患侧PWF无明显差异(P>0.05)、PWL仅在CFA注射后1 d有差异(P<0.05),其余差异无统计学意义(P>0.05);(2)CFA注射后1天,与注射生理盐水的WT小鼠组比较,WT小鼠DRG和脊髓背角内CGRP显著增加(P<0.05),注射生理盐水的KO小鼠DRG和脊髓背角内CGRP显著降低(P<0.05),而注射CFA的KO小鼠DRG和脊髓背角内CGRP无明显变化(P>0.05)。结论MBD1敲除缓解CFA诱导的机械和热刺激痛觉过敏,机制可能与抑制DRG和脊髓背角内CGRP生成和释放有关。展开更多
The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One pr...The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein（sorcin）. Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.展开更多
文摘目的探讨甲基-CpG结合蛋白1(methyl-CpG-binding domain protein 1,MBD1)缺失小鼠缓解弗氏完全佐剂(CFA)诱导的炎性疼痛是否通过抑制神经传导通路中的降钙素基因相关肽(CGRP)来实现的,为MBD1调控炎性疼痛的神经生物学机制提供新的思路。方法(1)取8周龄雄性MBD1敲除小鼠(KO)和与KO小鼠年龄、性别和体质量配对C57BL6小鼠(WT),按随机数字表法分为4组,分别为做足底注射生理盐水(NS)、CFA的WT小鼠组和注射NS、CFA的KO小鼠组(每组7只)。观察药物前1天(-1 d)、注射后2 h、1、3、7和14 d 4组小鼠机械缩足反应频率(PWF)和热缩足反应潜伏期(PWL)行为改变;(2)另取CFA注射后1 d(疼痛最明显点)上述组小鼠(每组3只),分别提取患侧背根神经节(DRG)和脊髓背角组织行ELISA法检测CGRP含量,比较4组含量变化。结果(1)与WT小鼠比较,KO小鼠CFA注射前明显降低双侧后爪基础PWF和延长PWL;与注射生理盐水的WT小鼠组比较,CFA注射后2 h、1、3、7和14 d WT小鼠患侧PWF增加(P<0.05)、PWL降低(P<0.05),而注射CFA的KO小鼠患侧PWF无明显差异(P>0.05)、PWL仅在CFA注射后1 d有差异(P<0.05),其余差异无统计学意义(P>0.05);(2)CFA注射后1天,与注射生理盐水的WT小鼠组比较,WT小鼠DRG和脊髓背角内CGRP显著增加(P<0.05),注射生理盐水的KO小鼠DRG和脊髓背角内CGRP显著降低(P<0.05),而注射CFA的KO小鼠DRG和脊髓背角内CGRP无明显变化(P>0.05)。结论MBD1敲除缓解CFA诱导的机械和热刺激痛觉过敏,机制可能与抑制DRG和脊髓背角内CGRP生成和释放有关。
文摘The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein（sorcin）. Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.
