The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave ...The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper,the long-term stability of the PPESO3 and the PPESO3 mixed with trypsin was studied,and the detection of proteolytic cleavage of bovine serum albumin(BSA) was obtained satisfactory results. The PPESO3 was provided a novel,simple,quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile,the fluorescent detection devices can be integrated into the microwave assisted protein digestion system,for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.展开更多
The microwave-assisted extraction(MAE)technology of tyrosinase inhibitor from batryticated silkworms was studied with the yield and the relative inhibitory activity against tyrosinase(RIAAT) of the extract used as eva...The microwave-assisted extraction(MAE)technology of tyrosinase inhibitor from batryticated silkworms was studied with the yield and the relative inhibitory activity against tyrosinase(RIAAT) of the extract used as evaluating indexes in the experiment.The effects of extraction variables,especially solvent concentrations,microwave power,the duration of microwave irradiation,materials-solvent mass ratio and material particle size on extraction efficiency were investigated.It was concluded that the optimal condition was two doses of microwave radiation(450 W) for 7 min with a mass ratio of 70 % ethanol to material by 8∶1.The extract of batryticated silkworms with 15.8 % yield and 66.7 % RIAAT was obtained after the extracted liquor was decoloured with active char,concentrated and then dried under vacuum.Compared with direct-heating extraction(DHE),MAE can shorten extracting duration,improve the yield and RIAAT of the extract.展开更多
文摘采用微波谐振腔对细胞色素c以及牛血清白蛋白进行微波辅助酶解,通过电喷雾三级四极杆质谱对得到的肽段进行分析,证明该方法可用很低的微波功率将蛋白质彻底酶解为多肽.通过调整微波条件可以使蛋白质的酶解效率基本达到100%,细胞色素c和牛血清白蛋白的序列覆盖率分别为45%和26%.该方法不但可将蛋白酶解时间由传统方法的16 h缩短为20min,还将功率由使用微波炉时的数百瓦降至20 W.
文摘The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper,the long-term stability of the PPESO3 and the PPESO3 mixed with trypsin was studied,and the detection of proteolytic cleavage of bovine serum albumin(BSA) was obtained satisfactory results. The PPESO3 was provided a novel,simple,quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile,the fluorescent detection devices can be integrated into the microwave assisted protein digestion system,for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.
文摘The microwave-assisted extraction(MAE)technology of tyrosinase inhibitor from batryticated silkworms was studied with the yield and the relative inhibitory activity against tyrosinase(RIAAT) of the extract used as evaluating indexes in the experiment.The effects of extraction variables,especially solvent concentrations,microwave power,the duration of microwave irradiation,materials-solvent mass ratio and material particle size on extraction efficiency were investigated.It was concluded that the optimal condition was two doses of microwave radiation(450 W) for 7 min with a mass ratio of 70 % ethanol to material by 8∶1.The extract of batryticated silkworms with 15.8 % yield and 66.7 % RIAAT was obtained after the extracted liquor was decoloured with active char,concentrated and then dried under vacuum.Compared with direct-heating extraction(DHE),MAE can shorten extracting duration,improve the yield and RIAAT of the extract.
