A bioreactor system with a total working volume of 4 8 L,composed of four airlift suspended bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was esta...A bioreactor system with a total working volume of 4 8 L,composed of four airlift suspended bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was established and continuously operated for 40 days A self flocculating fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe (SPSC 01) was used and ethanol and yeast were co produced from corn dry milling, two stage enzymatic hydrolysis (raw material residues were separated after saccharification).The results showed that average levels of 98 g·L -1 for ethanol, 4 g·L -1 for residual reduced sugar and 6—9 g·L -1 for residual total sugar were achieved when the medium containing total sugar of 220 g·L -1 was fed at a dilution rate of 0 05 h -1 .Pure yeast, equivalent to 5% (mass) of ethanol and containing protein as high as 48 6%(mass), was co produced Ethanol production is expected to be more economically competitive as this high quality yeast potentially can be further processed as value added by products.展开更多
文摘A bioreactor system with a total working volume of 4 8 L,composed of four airlift suspended bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was established and continuously operated for 40 days A self flocculating fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe (SPSC 01) was used and ethanol and yeast were co produced from corn dry milling, two stage enzymatic hydrolysis (raw material residues were separated after saccharification).The results showed that average levels of 98 g·L -1 for ethanol, 4 g·L -1 for residual reduced sugar and 6—9 g·L -1 for residual total sugar were achieved when the medium containing total sugar of 220 g·L -1 was fed at a dilution rate of 0 05 h -1 .Pure yeast, equivalent to 5% (mass) of ethanol and containing protein as high as 48 6%(mass), was co produced Ethanol production is expected to be more economically competitive as this high quality yeast potentially can be further processed as value added by products.
