A combined gas chromatography combustion-isotope ratio mass spectrometry method(GC-C IRMS) for stable carbon isotope analysis of amino acids is presented. Unlike hydrocarbons, amino acids require derivatization prior ...A combined gas chromatography combustion-isotope ratio mass spectrometry method(GC-C IRMS) for stable carbon isotope analysis of amino acids is presented. Unlike hydrocarbons, amino acids require derivatization prior to GC-C-IRMS analysis. Replicate carbon isotope analyses of trifluoroacetyl isopropyl ester derivatives of 17 amino acids by IRMS revealed that the derivatization process is reproducible. Due to a reproducible isotopic fractionation an empirical correction factor for each individual amino acid is derived separately for derivatives and the original δ^13C value of the underivatized amino acid is calculated.展开更多
文摘A combined gas chromatography combustion-isotope ratio mass spectrometry method(GC-C IRMS) for stable carbon isotope analysis of amino acids is presented. Unlike hydrocarbons, amino acids require derivatization prior to GC-C-IRMS analysis. Replicate carbon isotope analyses of trifluoroacetyl isopropyl ester derivatives of 17 amino acids by IRMS revealed that the derivatization process is reproducible. Due to a reproducible isotopic fractionation an empirical correction factor for each individual amino acid is derived separately for derivatives and the original δ^13C value of the underivatized amino acid is calculated.
