With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a c...With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.展开更多
Electrochemical processes lie at the core of biological function,governing energy transduction,metabolic flux,and mo-lecular signaling.Recent advances in electrochemical science now allow these processes to be probed ...Electrochemical processes lie at the core of biological function,governing energy transduction,metabolic flux,and mo-lecular signaling.Recent advances in electrochemical science now allow these processes to be probed and controlled with unprecedented spatial,temporal,and chemical resolution.In this review,we present an integrated framework that pro-gresses from fundamental mechanisms to analytical technologies and functional modulation.We begin by outlining elec-tron transfer pathways in mitochondrial respiration,microbial extracellular electron transfer,and DNA-and protein-based charge conduction,followed by the principles of photon-electron conversion in photosynthesis and the central role of redox equilibrium in coordinating cellular responses.We then highlight electrochemical analytical strategies that enable multiscale biological characterization,including biosensing,electrochemical and scanning probe imaging,electrogenerated chemilu-minescence detection,and measurements of membrane potentials and neurotransmitter dynamics.Emerging platforms such as flexible biointerfaces,ultramicroelectrodes,and nanopore systems further extend these capabilities to in vivo and single-molecule contexts.Finally,we discuss how electrochemical inputs can be used to regulate metabolic pathways,mi-crobial and protein activities,and neural signaling,enabling precision therapeutic and bioengineering applications.Togeth-er,these developments establish electrochemistry as a powerful foundation for decoding and directing biological systems.展开更多
Effects of working parameters on performance characteristics of hydrostatic turntable are researched by applying the fluid-structure-thermal coupled model.Fluid-structure interaction(FSI)technique and computational fl...Effects of working parameters on performance characteristics of hydrostatic turntable are researched by applying the fluid-structure-thermal coupled model.Fluid-structure interaction(FSI)technique and computational fluid dynamics(CFD)method are both employed by this new model,and thermal effects are also considered.Hydrostatic turntable systems with a series of oil supply pressures,various oil recess depth and several surface roughness parameters are studied.Performance parameters,such as turntable displacement,system flow rate,temperature rise of lubrication,stiffness and damping coefficients,are derived from different working parameters(rotational speed of turntable and exerted external load)of the hydrostatic turntable.Numerical results obtained from this FSI-thermal model are presented and discussed,and theoretical predictions are in good agreement with the experimental data.Therefore,this developed model is a very useful tool for studying hydrostatic turntables.The calculation results show that in order to obtain better performance,a rational selection of the design parameters is essential.展开更多
Though high accuracy of the thermal contour was obtained while adopting finite element method,it could not meet the real-time requirement.One-dimensional finite difference method could realize real-time control in the...Though high accuracy of the thermal contour was obtained while adopting finite element method,it could not meet the real-time requirement.One-dimensional finite difference method could realize real-time control in the case of neglecting both the circumferential and radical heat exchange,but the over-simplified modes resulted in poor accuracy.And two-dimensional full explicit difference was also limited in practical application since its time step was restricted to keep the model stable.Consequently,a new method of alternating direction finite difference was introduced and discussed in the model's stability,calculating speed and precision.Specific work roll after one real rolling unit was researched,The result shows that error of temperature on work roll surface between measured and calculated values is within 5 ℃.The influence of rolling rhythm and strip width on thermal crown was also studied.The conclusion is verified theoretically and practically that it can maintain absolutely stable and meet the online requirement.展开更多
The behavior of matrix converter(MC) drive systems under the condition of MC short-circuit faults is comprehensively investigated. Two isolation strategies using semiconductors and high speed fuses(HSFs) for MC short-...The behavior of matrix converter(MC) drive systems under the condition of MC short-circuit faults is comprehensively investigated. Two isolation strategies using semiconductors and high speed fuses(HSFs) for MC short-circuit faults are examined and their performances are compared. The behavior of MC drive systems during the fuse action time under different operating conditions is explored. The feasibility of fault-tolerant operation during the fuse action time is also studied. The basic selection laws for the HSFs and the requirements for the passive components of the MC drive system from the point view of short-circuit faults are also discussed. Simulation results are used to demonstrate the feasibility of the proposed isolation strategies.展开更多
The wear rate between the rotors of a hypotrochoidal gear pump is characterized.Using the knowledge of shape design on the rotors,the contact stresses without hydrodynamic effect between the rotor teeth were evaluated...The wear rate between the rotors of a hypotrochoidal gear pump is characterized.Using the knowledge of shape design on the rotors,the contact stresses without hydrodynamic effect between the rotor teeth were evaluated through the calculation of the Hertzian contact stress.Based on the above results and the sliding velocity between the rotors,a genetic algorithm (GA) was used as an optimization technique forminimizing the wear rate proportional factor (WRPF).The result shows that the wear rate or the WRPF can be reduced considerably,e.g.approximately 12.8%,throughout the optimization using GA.展开更多
The vehicle model of the recirculating ball-type electric power steering (EPS) system for the pure electric bus was built. According to the features of constrained optimization for multi-variable function, a multi-obj...The vehicle model of the recirculating ball-type electric power steering (EPS) system for the pure electric bus was built. According to the features of constrained optimization for multi-variable function, a multi-objective genetic algorithm (GA) was designed. Based on the model of system, the quantitative formula of the road feel, sensitivity, and operation stability of the steering were induced. Considering the road feel and sensitivity of steering as optimization objectives, and the operation stability of steering as constraint, the multi-objective GA was proposed and the system parameters were optimized. The simulation results show that the system optimized by multi-objective genetic algorithm has better road feel, steering sensibility and steering stability. The energy of steering road feel after optimization is 1.44 times larger than the one before optimization, and the energy of portability after optimization is 0.4 times larger than the one before optimization. The ground test was conducted in order to verify the feasibility of simulation results, and it is shown that the pure electric bus equipped with the recirculating ball-type EPS system can provide better road feel and better steering portability for the drivers, thus the optimization methods can provide a theoretical basis for the design and optimization of the recirculating ball-type EPS system.展开更多
This work aims to provide a relationship of how the key operational variables of frother type and impeller speed affect the size of bubble （D32）. The study was performed using pilot-scale equipment （0.8 m^3） that ...This work aims to provide a relationship of how the key operational variables of frother type and impeller speed affect the size of bubble （D32）. The study was performed using pilot-scale equipment （0.8 m^3） that is up to two orders of magnitude larger than equipment used for studies performed to date by others, and incorporated the key process variables of frother type and impeller speed. The results show that each frother family exhibits a unique CCC95-HLB relationship dependent on n （number of C-atoms in alkyl group） and m （number of propylene oxide group）. Empirical models were developed to predict CCC95 from HLB associated with other two parameters a and ft. The impeller speed-bubble size tests show that D32 is unaffected by increased impeller tip speed across the range of 4.6 to 9.2 m/s （representing the industrial operating range）, although D32 starts to increase below 4.6 m/s. The finding is valid for both coalescing and non-coalescing conditions. The results suggest that the bubble size and bubble size distribution （BSD） being created do not change with increasing impeller speed in the quiescent zone of the flotation.展开更多
The present work deals with an investigation of the self-starting aerodynamic characteristics of VAWT under fluctuating wind. In contrast to the previous studies, the rotational speed of the turbine is not fixed, the ...The present work deals with an investigation of the self-starting aerodynamic characteristics of VAWT under fluctuating wind. In contrast to the previous studies, the rotational speed of the turbine is not fixed, the rotation of the turbine is determined by the dynamic interaction between the fluctuating wind and turbine. A weak coupling method is developed to simulate the dynamic interaction between the fluctuating wind and passive rotation turbine, and the results show that if the fluctuating wind with appropriate fluctuation amplitude and frequency, the self-starting aerodynamic characteristics of VAWT will be enhanced. It is also found that compared with the fluctuation amplitude, the fluctuation frequency of the variation in wind velocity is shown to have a minor effect on the performance of the turbine. The analysis will provide straightforward physical insight into the self-starting aerodynamic characteristics of VAWT under fluctuating wind.展开更多
基金the Experimental Technology Research Project of Zhejiang University(SYB202138)National Natural Science Foundation of China(32000195)。
文摘With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.
基金supported by the National Key Research and Development Program of China(Nos.2021YFA1200101 and 2021YFA1200104)the National Natural Science Foundation of China(Nos.21635005,21890741,22174134,and 22474133)the CAS Project for Young Scientists in Basic Research(YSBR-054).
文摘Electrochemical processes lie at the core of biological function,governing energy transduction,metabolic flux,and mo-lecular signaling.Recent advances in electrochemical science now allow these processes to be probed and controlled with unprecedented spatial,temporal,and chemical resolution.In this review,we present an integrated framework that pro-gresses from fundamental mechanisms to analytical technologies and functional modulation.We begin by outlining elec-tron transfer pathways in mitochondrial respiration,microbial extracellular electron transfer,and DNA-and protein-based charge conduction,followed by the principles of photon-electron conversion in photosynthesis and the central role of redox equilibrium in coordinating cellular responses.We then highlight electrochemical analytical strategies that enable multiscale biological characterization,including biosensing,electrochemical and scanning probe imaging,electrogenerated chemilu-minescence detection,and measurements of membrane potentials and neurotransmitter dynamics.Emerging platforms such as flexible biointerfaces,ultramicroelectrodes,and nanopore systems further extend these capabilities to in vivo and single-molecule contexts.Finally,we discuss how electrochemical inputs can be used to regulate metabolic pathways,mi-crobial and protein activities,and neural signaling,enabling precision therapeutic and bioengineering applications.Togeth-er,these developments establish electrochemistry as a powerful foundation for decoding and directing biological systems.
基金Projects (51175518,51705147) supported by the National Natural Science Foundation of China
文摘Effects of working parameters on performance characteristics of hydrostatic turntable are researched by applying the fluid-structure-thermal coupled model.Fluid-structure interaction(FSI)technique and computational fluid dynamics(CFD)method are both employed by this new model,and thermal effects are also considered.Hydrostatic turntable systems with a series of oil supply pressures,various oil recess depth and several surface roughness parameters are studied.Performance parameters,such as turntable displacement,system flow rate,temperature rise of lubrication,stiffness and damping coefficients,are derived from different working parameters(rotational speed of turntable and exerted external load)of the hydrostatic turntable.Numerical results obtained from this FSI-thermal model are presented and discussed,and theoretical predictions are in good agreement with the experimental data.Therefore,this developed model is a very useful tool for studying hydrostatic turntables.The calculation results show that in order to obtain better performance,a rational selection of the design parameters is essential.
基金Project(2006BAE03A13) supported by the National Science and Technology Support Program during the 11th Five-Year Plan Period
文摘Though high accuracy of the thermal contour was obtained while adopting finite element method,it could not meet the real-time requirement.One-dimensional finite difference method could realize real-time control in the case of neglecting both the circumferential and radical heat exchange,but the over-simplified modes resulted in poor accuracy.And two-dimensional full explicit difference was also limited in practical application since its time step was restricted to keep the model stable.Consequently,a new method of alternating direction finite difference was introduced and discussed in the model's stability,calculating speed and precision.Specific work roll after one real rolling unit was researched,The result shows that error of temperature on work roll surface between measured and calculated values is within 5 ℃.The influence of rolling rhythm and strip width on thermal crown was also studied.The conclusion is verified theoretically and practically that it can maintain absolutely stable and meet the online requirement.
基金Project(50807002) supported by the National Natural Science Foundation of ChinaProject(SKLD10KM05) supported by Opening Fund of State Key Laboratory of Power System and Generation EquipmentsProject(201206025007) supported by the National Scholarship Fund,China
文摘The behavior of matrix converter(MC) drive systems under the condition of MC short-circuit faults is comprehensively investigated. Two isolation strategies using semiconductors and high speed fuses(HSFs) for MC short-circuit faults are examined and their performances are compared. The behavior of MC drive systems during the fuse action time under different operating conditions is explored. The feasibility of fault-tolerant operation during the fuse action time is also studied. The basic selection laws for the HSFs and the requirements for the passive components of the MC drive system from the point view of short-circuit faults are also discussed. Simulation results are used to demonstrate the feasibility of the proposed isolation strategies.
基金supported by Changwon National University in 2010,Korea
文摘The wear rate between the rotors of a hypotrochoidal gear pump is characterized.Using the knowledge of shape design on the rotors,the contact stresses without hydrodynamic effect between the rotor teeth were evaluated through the calculation of the Hertzian contact stress.Based on the above results and the sliding velocity between the rotors,a genetic algorithm (GA) was used as an optimization technique forminimizing the wear rate proportional factor (WRPF).The result shows that the wear rate or the WRPF can be reduced considerably,e.g.approximately 12.8%,throughout the optimization using GA.
基金Projects(51005115, 51005248) supported by the National Natural Science Foundation of ChinaProject(SKLMT-KFKT-201105)supported by the Visiting Scholar Foundation of State Key Laboratory of Mechanical Transmission in Chongqing University, ChinaProject(QC201101) supported by Visiting Scholar Foundation of the Automobile Engineering Key Laboratory of Jiangsu Province, China
文摘The vehicle model of the recirculating ball-type electric power steering (EPS) system for the pure electric bus was built. According to the features of constrained optimization for multi-variable function, a multi-objective genetic algorithm (GA) was designed. Based on the model of system, the quantitative formula of the road feel, sensitivity, and operation stability of the steering were induced. Considering the road feel and sensitivity of steering as optimization objectives, and the operation stability of steering as constraint, the multi-objective GA was proposed and the system parameters were optimized. The simulation results show that the system optimized by multi-objective genetic algorithm has better road feel, steering sensibility and steering stability. The energy of steering road feel after optimization is 1.44 times larger than the one before optimization, and the energy of portability after optimization is 0.4 times larger than the one before optimization. The ground test was conducted in order to verify the feasibility of simulation results, and it is shown that the pure electric bus equipped with the recirculating ball-type EPS system can provide better road feel and better steering portability for the drivers, thus the optimization methods can provide a theoretical basis for the design and optimization of the recirculating ball-type EPS system.
基金Project supported by the Collaborative Research and Development Program of NSERC(Natural Sciences and Engineering Research Council of Canada) with Industrial Sponsorship from Vale,Teck Cominco,Xstrata Process Support,Agnico-Eagle,Shell Canada,Barrick Gold,COREM,SGS Lakefield Research and Flottec
文摘This work aims to provide a relationship of how the key operational variables of frother type and impeller speed affect the size of bubble （D32）. The study was performed using pilot-scale equipment （0.8 m^3） that is up to two orders of magnitude larger than equipment used for studies performed to date by others, and incorporated the key process variables of frother type and impeller speed. The results show that each frother family exhibits a unique CCC95-HLB relationship dependent on n （number of C-atoms in alkyl group） and m （number of propylene oxide group）. Empirical models were developed to predict CCC95 from HLB associated with other two parameters a and ft. The impeller speed-bubble size tests show that D32 is unaffected by increased impeller tip speed across the range of 4.6 to 9.2 m/s （representing the industrial operating range）, although D32 starts to increase below 4.6 m/s. The finding is valid for both coalescing and non-coalescing conditions. The results suggest that the bubble size and bubble size distribution （BSD） being created do not change with increasing impeller speed in the quiescent zone of the flotation.
基金Projects(61105086,51505347)supported by the National Natural Science Foundation of China
文摘The present work deals with an investigation of the self-starting aerodynamic characteristics of VAWT under fluctuating wind. In contrast to the previous studies, the rotational speed of the turbine is not fixed, the rotation of the turbine is determined by the dynamic interaction between the fluctuating wind and turbine. A weak coupling method is developed to simulate the dynamic interaction between the fluctuating wind and passive rotation turbine, and the results show that if the fluctuating wind with appropriate fluctuation amplitude and frequency, the self-starting aerodynamic characteristics of VAWT will be enhanced. It is also found that compared with the fluctuation amplitude, the fluctuation frequency of the variation in wind velocity is shown to have a minor effect on the performance of the turbine. The analysis will provide straightforward physical insight into the self-starting aerodynamic characteristics of VAWT under fluctuating wind.
