An attempt had been made to isolate and identify the low polar volatile constituents from Isodon excisa. The dried plants were heat extracted with ethanol and then extracted with petroleum ether. The constituents were...An attempt had been made to isolate and identify the low polar volatile constituents from Isodon excisa. The dried plants were heat extracted with ethanol and then extracted with petroleum ether. The constituents were isolated and analyzed by GC-MS and then we retrieved them on NIST08 mass spectrometry database to determine their structures. Finally, successful results were achieved, 111 compounds were obtained and identified. 105 of them were reported for the first time in lsodon excisa and 22 of them were first found in natural products. Thus, the main constituents of it were found to be Sitosterol （33.28%）, 3-Amino-4-pyrazolecarbonitrile （9.55%）展开更多
To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the d...To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the differential expression proteins. Methods： Forty Kunming mice were randomly divided into infection group （20） injected with HCMVAD169 and control group （20） injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis（2-DE）, analyzed by Image Master 2D software, and identified by peptide mass fingerprint（PMF） and database searching, and make Western blotting analyses the differential expression of individual proteins. Results： Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry（MS） were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion： These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine.展开更多
文摘An attempt had been made to isolate and identify the low polar volatile constituents from Isodon excisa. The dried plants were heat extracted with ethanol and then extracted with petroleum ether. The constituents were isolated and analyzed by GC-MS and then we retrieved them on NIST08 mass spectrometry database to determine their structures. Finally, successful results were achieved, 111 compounds were obtained and identified. 105 of them were reported for the first time in lsodon excisa and 22 of them were first found in natural products. Thus, the main constituents of it were found to be Sitosterol （33.28%）, 3-Amino-4-pyrazolecarbonitrile （9.55%）
基金Supported by the Program of Science and Technology from Shenzhen City, Guangdong Province (200802051)
文摘To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the differential expression proteins. Methods： Forty Kunming mice were randomly divided into infection group （20） injected with HCMVAD169 and control group （20） injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis（2-DE）, analyzed by Image Master 2D software, and identified by peptide mass fingerprint（PMF） and database searching, and make Western blotting analyses the differential expression of individual proteins. Results： Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry（MS） were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion： These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine.
