对比减压和常压条件下提取的柴胡挥发油在成分、含量及药效方面的差异,考察挥发油的品质,优选提取方法。采用气相色谱-质谱联用(GC-MS)法分析柴胡挥发油成分及其相对含量,再采用气相色谱法建立挥发油中己醛、庚醛、正辛醛和反式-2,4-癸...对比减压和常压条件下提取的柴胡挥发油在成分、含量及药效方面的差异,考察挥发油的品质,优选提取方法。采用气相色谱-质谱联用(GC-MS)法分析柴胡挥发油成分及其相对含量,再采用气相色谱法建立挥发油中己醛、庚醛、正辛醛和反式-2,4-癸二烯醛的含量检测方法,测定不同压力下提取的柴胡挥发油有效成分含量,通过背部皮下注射酵母混悬液复制大鼠发热模型,将常压、减压提取的柴胡挥发油溶于1%吐温80后腹腔注射给药,测定大鼠不同时间点的体温变化情况,利用单因素方差分析(Single factor analysis of variance)进行组间比较。结果显示:减压提取与常压提取的挥发油成分和含量存在差异,己醛、庚醛、正辛醛和反式-2,4-癸二烯醛在减压提取方法中的提取率更高,同时该方法提取的挥发油对干酵母引起的发热药效更显著。由此可见,减压提取能有效减少热敏性成分被破坏,提高有效成分的提取效率,提取的柴胡挥发油具有更显著的药效,品质优于常压提取。展开更多
The elevated supersaturation of total dissolved gas （TDG） downstream of a high-dam spill has deleterious effects on fish in a large range. A one-dimensional （l-D） longitudinal model is optimal for the prediction o...The elevated supersaturation of total dissolved gas （TDG） downstream of a high-dam spill has deleterious effects on fish in a large range. A one-dimensional （l-D） longitudinal model is optimal for the prediction of supersaturated TDG dissipation over a long distance. The key issue of the model is to determine the dissipation coefficient accurately. In agreement with field observations and experiment data, dimensional analysis and regression were performed to propose a formula for estimating the dissipation coefficient of supersaturated TDG in various rivers and reservoirs, and it involves the effects of the turbulence intensity, the hydro-pressure and the solid-liquid interface. The friction velocity, water depth, hydraulic radius and Froude number are independent variables in the formula which are easy to determine in practical applications. The 1-D longitudinal model is implemented to calculate the dissipation of TDG in a reach of the Jinsha River. Good agreement is found between the calculated results and field data for both the dissipation coefficient and the dissipation process.展开更多
文摘对比减压和常压条件下提取的柴胡挥发油在成分、含量及药效方面的差异,考察挥发油的品质,优选提取方法。采用气相色谱-质谱联用(GC-MS)法分析柴胡挥发油成分及其相对含量,再采用气相色谱法建立挥发油中己醛、庚醛、正辛醛和反式-2,4-癸二烯醛的含量检测方法,测定不同压力下提取的柴胡挥发油有效成分含量,通过背部皮下注射酵母混悬液复制大鼠发热模型,将常压、减压提取的柴胡挥发油溶于1%吐温80后腹腔注射给药,测定大鼠不同时间点的体温变化情况,利用单因素方差分析(Single factor analysis of variance)进行组间比较。结果显示:减压提取与常压提取的挥发油成分和含量存在差异,己醛、庚醛、正辛醛和反式-2,4-癸二烯醛在减压提取方法中的提取率更高,同时该方法提取的挥发油对干酵母引起的发热药效更显著。由此可见,减压提取能有效减少热敏性成分被破坏,提高有效成分的提取效率,提取的柴胡挥发油具有更显著的药效,品质优于常压提取。
基金Projects(51179111,51279115)supported by the National Natural Science Foundation of ChinaProject(20110181110073)supported by the Doctoral Programs Foundation of Ministry of Education of China
文摘The elevated supersaturation of total dissolved gas （TDG） downstream of a high-dam spill has deleterious effects on fish in a large range. A one-dimensional （l-D） longitudinal model is optimal for the prediction of supersaturated TDG dissipation over a long distance. The key issue of the model is to determine the dissipation coefficient accurately. In agreement with field observations and experiment data, dimensional analysis and regression were performed to propose a formula for estimating the dissipation coefficient of supersaturated TDG in various rivers and reservoirs, and it involves the effects of the turbulence intensity, the hydro-pressure and the solid-liquid interface. The friction velocity, water depth, hydraulic radius and Froude number are independent variables in the formula which are easy to determine in practical applications. The 1-D longitudinal model is implemented to calculate the dissipation of TDG in a reach of the Jinsha River. Good agreement is found between the calculated results and field data for both the dissipation coefficient and the dissipation process.
