目的探讨表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)技术在筛选神经管缺陷胎儿母亲尿液中蛋白质标志物的应用。方法经超声确诊为神经管缺陷胎儿的孕妇20例和正常孕妇15例。神经管缺陷包括脊柱裂10例,无脑儿5例,脑积水5例。所有...目的探讨表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)技术在筛选神经管缺陷胎儿母亲尿液中蛋白质标志物的应用。方法经超声确诊为神经管缺陷胎儿的孕妇20例和正常孕妇15例。神经管缺陷包括脊柱裂10例,无脑儿5例,脑积水5例。所有孕妇均取清晨空腹尿5ml,离心后取上清,选用CM10芯片检测。采用PBSⅡC型蛋白质芯片阅读机读取数据。设定优化相对分子质量范围1000～30000Da。数据分析使用Ciphergen protein chip 3.1.1软件。结果共检测到39个尿液差异蛋白质峰,有统计学意义的差异蛋白质峰5个。病例组中有4种蛋白质高表达,相对分子质量分别为8320,8209,9099,10567;1种蛋白质低表达,相对分子质量3458。建立决策分类树,得到带有3个终结点的决策分类树,获得2个标志性蛋白质,分子量为9096,8244。进行留一法交叉验证后,神经管缺陷诊断模型的灵敏度为80.0%,特异度为93.3%。结论应用SELDI-TOF-MS技术可以在孕妇尿液中筛查出神经管缺陷的特异性蛋白质标志物,进一步可用于神经管缺陷产前早期无创诊断。展开更多
表面辅助激光解吸附/离子化质谱(Surface-assisted laser desorption/ionization mass spectrometry,SALDI-MS)是一种利用无机纳米粒子或纳米结构表面作为基质,辅助待测分子的解吸附和离子化的质谱技术。由于其具有灵敏度高、耐盐性好...表面辅助激光解吸附/离子化质谱(Surface-assisted laser desorption/ionization mass spectrometry,SALDI-MS)是一种利用无机纳米粒子或纳米结构表面作为基质,辅助待测分子的解吸附和离子化的质谱技术。由于其具有灵敏度高、耐盐性好、操作简便、重现性好、检测通量高等优势,已经被广泛应用于食品安全、环境监测、生命科学等诸多领域。该文总结了近5年来,SALDI基质材料(金属及金属氧化物材料、碳材料、硅材料、金属有机骨架化合物材料等)的最新研究进展及其在生物检测领域中的应用,并对SALDI-MS基质材料的发展及应用进行了展望。展开更多
Changes to the microstructure of a hard carbon(HC)and its solid electrolyte interface(SEI)can be effective in improving the electrode kinetics.However,achieving fast charging using a simple and inexpensive strategy wi...Changes to the microstructure of a hard carbon(HC)and its solid electrolyte interface(SEI)can be effective in improving the electrode kinetics.However,achieving fast charging using a simple and inexpensive strategy without sacrificing its initial Coulombic efficiency remains a challenge in sodium ion batteries.A simple liquid-phase coating approach has been used to generate a pitch-derived soft carbon layer on the HC surface,and its effect on the porosity of HC and SEI chemistry has been studied.A variety of structural characterizations show a soft carbon coating can increase the defect and ultra-micropore contents.The increase in ultra-micropore comes from both the soft carbon coatings and the larger pores within the HC that are partially filled by pitch,which provides more Na+storage sites.In-situ FTIR/EIS and ex-situ XPS showed that the soft carbon coating induced the formation of thinner SEI that is richer in NaF from the electrolyte,which stabilized the interface and promoted the charge transfer process.As a result,the anode produced fastcharging(329.8 mAh g^(−1)at 30 mA g^(−1)and 198.6 mAh g^(−1)at 300 mA g^(−1))and had a better cycling performance(a high capacity retention of 81.4%after 100 cycles at 150 mA g^(−1)).This work reveals the critical role of coating layer in changing the pore structure,SEI chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced fast charging capability.展开更多
Metal nanoparticles with high surface area and high electrochemical activity exhibit excellent catalytic performance in the photocatalytic reduction of carbon dioxide(CO_(2)).However,poor stability,small specific surf...Metal nanoparticles with high surface area and high electrochemical activity exhibit excellent catalytic performance in the photocatalytic reduction of carbon dioxide(CO_(2)).However,poor stability,small specific surface area,and less active sites limits its solar energy utilization.Hydrothermal method was utilized to synthesize the bimetallic material of Cu_(x)Co_(1-x)in this work.Co was loaded onto the Cu surface due to the electrons generated by the surface plasmon resonance(SPR)effect occurring on the Cu surface.Cu_(x)Co_(1-x)exhibits high photocatalytic conversion of CO_(2)efficiency under irradiation,which mainly because the Co nanoparticles on the surface of Cu can be used as cocatalysts to enhance the photocharge transfer.Cu_(0.6)Co_(0.4)exhibits the comparatively best photocatalytic conversion efficiency of CO_(2)in the first 6 h light irradiation.The yields of CO and CH_(4)reached 35.26 and 2.71μmol/(g·h),respectively.Upon illumination,electrons were produced,with the majority of them moving towards the interface.This movement contributes to the increased lifetime of photogenerated electron-hole pairs,which in turn boosts the photocatalytic efficiency.The findings of this research provide significant insights for creating photocatalysts that are both highly effective and stable in CO_(2)reduction processes.展开更多
文摘目的探讨表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)技术在筛选神经管缺陷胎儿母亲尿液中蛋白质标志物的应用。方法经超声确诊为神经管缺陷胎儿的孕妇20例和正常孕妇15例。神经管缺陷包括脊柱裂10例,无脑儿5例,脑积水5例。所有孕妇均取清晨空腹尿5ml,离心后取上清,选用CM10芯片检测。采用PBSⅡC型蛋白质芯片阅读机读取数据。设定优化相对分子质量范围1000～30000Da。数据分析使用Ciphergen protein chip 3.1.1软件。结果共检测到39个尿液差异蛋白质峰,有统计学意义的差异蛋白质峰5个。病例组中有4种蛋白质高表达,相对分子质量分别为8320,8209,9099,10567;1种蛋白质低表达,相对分子质量3458。建立决策分类树,得到带有3个终结点的决策分类树,获得2个标志性蛋白质,分子量为9096,8244。进行留一法交叉验证后,神经管缺陷诊断模型的灵敏度为80.0%,特异度为93.3%。结论应用SELDI-TOF-MS技术可以在孕妇尿液中筛查出神经管缺陷的特异性蛋白质标志物,进一步可用于神经管缺陷产前早期无创诊断。
文摘表面辅助激光解吸附/离子化质谱(Surface-assisted laser desorption/ionization mass spectrometry,SALDI-MS)是一种利用无机纳米粒子或纳米结构表面作为基质,辅助待测分子的解吸附和离子化的质谱技术。由于其具有灵敏度高、耐盐性好、操作简便、重现性好、检测通量高等优势,已经被广泛应用于食品安全、环境监测、生命科学等诸多领域。该文总结了近5年来,SALDI基质材料(金属及金属氧化物材料、碳材料、硅材料、金属有机骨架化合物材料等)的最新研究进展及其在生物检测领域中的应用,并对SALDI-MS基质材料的发展及应用进行了展望。
基金National Key Research and Development Program of China(2022YFE0206300)National Natural Science Foundation of China(U21A2081,22075074,22209047)+2 种基金Guangdong Basic and Applied Basic Research Foundation(2024A1515011620)Hunan Provincial Natural Science Foundation of China(2024JJ5068)Foundation of Yuelushan Center for Industrial Innovation(2023YCII0119)。
文摘Changes to the microstructure of a hard carbon(HC)and its solid electrolyte interface(SEI)can be effective in improving the electrode kinetics.However,achieving fast charging using a simple and inexpensive strategy without sacrificing its initial Coulombic efficiency remains a challenge in sodium ion batteries.A simple liquid-phase coating approach has been used to generate a pitch-derived soft carbon layer on the HC surface,and its effect on the porosity of HC and SEI chemistry has been studied.A variety of structural characterizations show a soft carbon coating can increase the defect and ultra-micropore contents.The increase in ultra-micropore comes from both the soft carbon coatings and the larger pores within the HC that are partially filled by pitch,which provides more Na+storage sites.In-situ FTIR/EIS and ex-situ XPS showed that the soft carbon coating induced the formation of thinner SEI that is richer in NaF from the electrolyte,which stabilized the interface and promoted the charge transfer process.As a result,the anode produced fastcharging(329.8 mAh g^(−1)at 30 mA g^(−1)and 198.6 mAh g^(−1)at 300 mA g^(−1))and had a better cycling performance(a high capacity retention of 81.4%after 100 cycles at 150 mA g^(−1)).This work reveals the critical role of coating layer in changing the pore structure,SEI chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced fast charging capability.
基金supported by the Doctoral Research Start-up Project of Yuncheng University(YQ-2023067)Project of Shanxi Natural Science Foundation(202303021211189)+2 种基金Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Provinces(20220036)Shanxi Province Intelligent Optoelectronic Sensing Application Technology Innovation CenterShanxi Province Optoelectronic Information Science and Technology Laboratory,Yuncheng University。
文摘Metal nanoparticles with high surface area and high electrochemical activity exhibit excellent catalytic performance in the photocatalytic reduction of carbon dioxide(CO_(2)).However,poor stability,small specific surface area,and less active sites limits its solar energy utilization.Hydrothermal method was utilized to synthesize the bimetallic material of Cu_(x)Co_(1-x)in this work.Co was loaded onto the Cu surface due to the electrons generated by the surface plasmon resonance(SPR)effect occurring on the Cu surface.Cu_(x)Co_(1-x)exhibits high photocatalytic conversion of CO_(2)efficiency under irradiation,which mainly because the Co nanoparticles on the surface of Cu can be used as cocatalysts to enhance the photocharge transfer.Cu_(0.6)Co_(0.4)exhibits the comparatively best photocatalytic conversion efficiency of CO_(2)in the first 6 h light irradiation.The yields of CO and CH_(4)reached 35.26 and 2.71μmol/(g·h),respectively.Upon illumination,electrons were produced,with the majority of them moving towards the interface.This movement contributes to the increased lifetime of photogenerated electron-hole pairs,which in turn boosts the photocatalytic efficiency.The findings of this research provide significant insights for creating photocatalysts that are both highly effective and stable in CO_(2)reduction processes.
