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酶解菌质水溶性膳食纤维表面显微结构及成分研究 被引量:2
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作者 余有贵 李忠海 《食品与机械》 CSCD 北大核心 2014年第1期34-36,61,共4页
为研究菌质水溶性膳食纤维的基本结构特征,采用电子显微镜观察法、高效液相色谱法和气相色谱法对酶解菌质水溶性膳食纤维的表面显微结构及成分进行研究。结果表明:菌质水溶性膳食纤维表面有许多皱折,形成多孔的蜂窝状空腔和明显的裂缝;... 为研究菌质水溶性膳食纤维的基本结构特征,采用电子显微镜观察法、高效液相色谱法和气相色谱法对酶解菌质水溶性膳食纤维的表面显微结构及成分进行研究。结果表明:菌质水溶性膳食纤维表面有许多皱折,形成多孔的蜂窝状空腔和明显的裂缝;主要成分是葡萄糖、甘露糖、阿拉伯糖、半乳糖和木糖,其中葡萄糖高达92.45%;其相对分子量为1 772Da占68.75%、289Da占31.25%,菌质可溶性膳食纤维呈单分散的低聚糖。 展开更多
关键词 酶解 菌质 水溶性膳食纤维 表面显微结构 组成成分 相对分子量
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氧化温度对ZrB_2-YAG-Al_2O_3复相陶瓷表面组成的影响 被引量:3
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作者 宋杰光 李养良 +4 位作者 潘东 纪岗昌 王芳 李伟 陈林燕 《兵器材料科学与工程》 CAS CSCD 2010年第5期21-23,共3页
二硼化锆具有许多优异性能,因此它的应用非常广泛。研究氧化温度对ZrB2-YAG-Al2O3复相陶瓷的表面组成及表面显微结构的影响。结果表明:从1 000~1 100℃和1 200~1 300℃的氧化增重率趋势较大,而在1 100~1 200℃的温度范围增重率很缓... 二硼化锆具有许多优异性能,因此它的应用非常广泛。研究氧化温度对ZrB2-YAG-Al2O3复相陶瓷的表面组成及表面显微结构的影响。结果表明:从1 000~1 100℃和1 200~1 300℃的氧化增重率趋势较大,而在1 100~1 200℃的温度范围增重率很缓慢。在氧化温度低于1 200℃,氧化后的复相陶瓷中有ZrO2和B2O3生成,表面显微结构较为致密,而在1 300℃氧化温度以上,可以生成All8B4O33,表面显微结构疏松。 展开更多
关键词 超高温陶瓷 二硼化锆 高温氧化 表面组成 表面显微结构
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In situ observation of surface structures of cardiovascular endothelial cells with atomic force microscope
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作者 Tong Yin Jin Luo +3 位作者 Ya-Min Ma Xiao-Long Ji Yu-Sheng Zhao Shi-Wen Wang 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2009年第3期178-181,共4页
Objective To observe the surface structures of cardiovascular endothelial cells in situ with atomic force microscope (AFM). Methods Fresh aorta and aortic valve were dissected from 10 healthy male New Zealand white ... Objective To observe the surface structures of cardiovascular endothelial cells in situ with atomic force microscope (AFM). Methods Fresh aorta and aortic valve were dissected from 10 healthy male New Zealand white rabbits. Before fixed in 1% formaldehyde, the fresh tissues were washed in the buffer phosphate solution. Under general microscope, the fixed aorta or valve was spread on the double side stick tape which had already been stuck on the glass slide. The intima of aorta or the aorta side of valve was towards upside, Then the specimen was dried under 37 degrees centigrade in an attemperator and was washed with pure water. After dried again, the specimen was loaded on the platform ofNanoScope Ⅲa AFM and was scanned in tapping mode with the scanning speed of 0.5 HZ. Results The surface structures of endothelial cell on the fixed and dried tissue could be observed clearly in situ with AFM. aortic endothelial cells were large, branched and arranged sparsely and parallel to the direction of blood flow, whereas endothelial cells on aorta valve surface were small, less branched and arranged intensively and vertical to the direction of blood flow. When the scanning range was dwindled, granular ultra-structures could be observed on the surface of endothelial cells, and, as the scanning range was dwindled further, fissure and convolution could be seen on the surface of granules from aortic endothelial cells. Centre cavity and surrounding swelling volcano-like structure could be seen on the surface of granules from endothelial cells of aortic valve. Conclusions It's feasible to observe the surface ultra-structures of cardiovascular endothelial cells in situ with AFM and morphological information provided by AFM might be of clinical value in future histopathological diagnosis(JGeriatr Cardiol2009; 6:178-181). 展开更多
关键词 atomic force microscope endothelial cell
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