将生长抑素(ss)与乙肝表面抗原(HBsAg)融合基因(ss/HBs)克隆到痘苗病毒表达质粒pGJP-5中,通过与痘苗病毒天坛株体内重组和蚀斑挑选技术获得融合基因的重组病毒,它保持痘苗病毒原有的感染性,并能表达出 ss 和 HBsAg 产物.表达产物呈表面...将生长抑素(ss)与乙肝表面抗原(HBsAg)融合基因(ss/HBs)克隆到痘苗病毒表达质粒pGJP-5中,通过与痘苗病毒天坛株体内重组和蚀斑挑选技术获得融合基因的重组病毒,它保持痘苗病毒原有的感染性,并能表达出 ss 和 HBsAg 产物.表达产物呈表面带 ss 决定簇的 HBsAg 杂合颗粒,并分泌到感染细胞之培养液中.杂合颗粒的大小和密度与报道的 HBsAg 颗粒相似。本文还对该重组痘苗病毒可能作为 ss 活载体苗的前景进行了讨论。展开更多
A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using ...A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.展开更多
文摘将生长抑素(ss)与乙肝表面抗原(HBsAg)融合基因(ss/HBs)克隆到痘苗病毒表达质粒pGJP-5中,通过与痘苗病毒天坛株体内重组和蚀斑挑选技术获得融合基因的重组病毒,它保持痘苗病毒原有的感染性,并能表达出 ss 和 HBsAg 产物.表达产物呈表面带 ss 决定簇的 HBsAg 杂合颗粒,并分泌到感染细胞之培养液中.杂合颗粒的大小和密度与报道的 HBsAg 颗粒相似。本文还对该重组痘苗病毒可能作为 ss 活载体苗的前景进行了讨论。
基金The Research Grants Council of the Hong Kong Special Adm inistration RegionChina(No.HKBU 2 0 5 4/98p) +1 种基金国家杰出青年基金 (批准号 :2 982 5 110 )湖南省自然科学基金 (批准号 :0 0 GKY10 10 )资助
文摘A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.
