The xylitol dehydrogenase(XDH)is a crucial enzyme involved in the xylose utilization in pentose⁃catabolizing yeasts and fungi.In addition to producing xylulose,XDH can also be employed to develop a biosensor for monit...The xylitol dehydrogenase(XDH)is a crucial enzyme involved in the xylose utilization in pentose⁃catabolizing yeasts and fungi.In addition to producing xylulose,XDH can also be employed to develop a biosensor for monitoring xylitol concentration.In this study,the gene encoding the thermophilic fungus Talaromyces emersonii XDH(TeXDH)was heterologously expressed in Escherichia coli BL21(DE3)at 16℃in the soluble form.Recombinant TeXDH with high purity was purified by using a Ni⁃NTA affinity column.Size⁃exclusion chromatography and SDS⁃PAGE analysis demonstrated that the puri⁃fied recombinant TeXDH exists as a native trimer with a molecular mass of approximately 116 kD,and is composed of three identical subunits,each with a molecular weight of around 39 kD.The TeXDH strictly preferred NAD^(+)as a coenzyme to NADP^(+).The optimal temperature and pH of the TeXDH were 40℃and 10.0,respectively.After EDTA treatment,the enzyme activity of TeXDH decreased to 43.26%of the initial enzyme activity,while the divalent metal ions Mg^(2+)or Ca^(2+)could recover the enzyme activity of TeXDH,reaching 103.32%and 110.69%of the initial enzyme activity,respectively,making them the optimal divalent metal ion cofactors for TeXDH enzyme.However,the divalent metal ions of Mn^(2+),Ni^(2+),Cu^(2+),Zn^(2+),Co^(2+),and Cd^(2+)significantly inhibited the activity of TeXDH.ICP⁃MS and molecular doc⁃king studies revealed that 1 mol/L of TeXDH bound 2 mol/L Zn^(2+)ions and 1 mol/L Mg^(2+)ion.Further⁃more,TeXDH exhibited a high specificity for xylitol,laying the foundation for the development of future xylitol biosensors.展开更多
To investigate the complex macro-mechanical properties of coal from a micro-mechanical perspective,we have conducted a series of micro-mechanical experiments on coal using a nano-indentation instrument.These experimen...To investigate the complex macro-mechanical properties of coal from a micro-mechanical perspective,we have conducted a series of micro-mechanical experiments on coal using a nano-indentation instrument.These experiments were conducted under both dynamic and static loading conditions,allowing us to gather the micro-mechanical parameters of coal for further analysis of its micro-mechanical heterogeneity using the box counting statistical method and the Weibull model.The research findings indicate that the load–displacement curves of the coal mass under the two different loading modes exhibit noticeable discreteness.This can be attributed to the stress concentration phenomenon caused by variations in the mechanical properties of the micro-units during the loading process of the coal mass.Consequently,there are significant fluctuations in the micro-mechanical parameters of the coal mass.Moreover,the mechanical heterogeneity of the coal at the nanoscale was confirmed based on the calculation results of the standard deviation coefficient and Weibull modulus of the coal body’s micromechanical parameters.These results reveal the influence of microstructural defects and minerals on the uniformity of the stress field distribution within the loaded coal body,as well as on the ductility characteristics of the micro-defect structure.Furthermore,there is a pronounced heterogeneity in the micromechanical parameters.Furthermore,we have established a relationship between the macro and micro elastic modulus of coal by applying the Mori-Tanaka homogenization method.This relationship holds great significance for revealing the micro-mechanical failure mechanism of coal.展开更多
Zinc-ion capacitors(ZICs),which consist of a capacitor-type electrode and a battery-type electrode,not only possess the high power density of supercapacitors and the high energy density of batteries,but also have othe...Zinc-ion capacitors(ZICs),which consist of a capacitor-type electrode and a battery-type electrode,not only possess the high power density of supercapacitors and the high energy density of batteries,but also have other advantages such as abundant resources,high safety and environmental friendliness.However,they still face problems such as insufficient specific capacitance,a short cycling life,and narrow operating voltage and temperature ranges,which are hindering their practical use.We provide a comprehensive overview of the fundamental theory of carbon-based ZICs and summarize recent research progress from three perspectives:the carbon cathode,electrolyte and zinc anode.The influence of the structure and surface chemical properties of the carbon materials on the capacitive performance of ZICs is considered together with theoretical guidance for advancing their development and practical use.展开更多
The current study comprehensively evaluates four different protein extraction methods based on urea,sodium dodecyl sulfate(SDS),anionic surfactants(BT),and total RNA extractor(Trizol),aiming to optimize the sample pre...The current study comprehensively evaluates four different protein extraction methods based on urea,sodium dodecyl sulfate(SDS),anionic surfactants(BT),and total RNA extractor(Trizol),aiming to optimize the sample preparation workflow for mass spectrometry-based proteomics.Using HeLa cells as an example,we found that the method employing the mass spectrometry-compatible surfactant BT reagent significantly reduces the total time consumed for protein extraction and minimizes protein losses during the sample preparation process.Further integrating the four protein extraction methods,we identified over 7000 proteins from HeLa cells without relying on pre-fractionation techniques,and 2990 of them were quantified using label-free quantification.It is worth noting that the BT and SDS methods demonstrate higher efficiency in extracting membrane proteins,while the Urea and Trizol methods are more effective in extracting proteins from nuclear and cytoplasmic fractions.In summary,this study provides a novel solution for deep proteome coverage,particularly in the context of cellular protein extraction,by integrating mass spectrometry-compatible surfactants with traditional extraction methods to effectively enhance protein identification numbers.展开更多
Based on the working principles of particle bed comminution, particles produced by high-pressure grinding rolls （HPGR） have surface properties different from particles produced by other grinding patterns, which exer...Based on the working principles of particle bed comminution, particles produced by high-pressure grinding rolls （HPGR） have surface properties different from particles produced by other grinding patterns, which exert great influence on mineral flotation. Flotation performances of calcite particles under different grinding patterns involving the use of HPGR, a jaw crusher, a dry ball mill, a wet ball mill, and a wet rod mill were studied using single mineral flotation tests. The surface properties of the particles under different grinding patterns were characterized to determine the flotation performance variation in terms of specific surface area, particle size distribution, AFM, XPS, and zeta potential. The results show that particles ground by HPGR exhibited improved flotation performance within the lower range of grinding fineness in both NaOL and dodecyl amine flotation systems compared to the particles prepared using other grinding patterns. Specific surface area, particle size distribution, surface roughness, Fe（III） contamination, binding energy, and zeta potential are greatly influenced by grinding patterns, which is the main cause of the flotation performance variation.展开更多
This experiment obtained different laser energy density(LED) by changing SLM molding process parameters.The surface morphology, surface quality, and microstructure of as-fabricated samples were studied. The effects of...This experiment obtained different laser energy density(LED) by changing SLM molding process parameters.The surface morphology, surface quality, and microstructure of as-fabricated samples were studied. The effects of scanning speed, hatching space, and laser power on surface quality were analyzed, and the optimal LED range for surface quality was determined. The results show that pores and spherical particles appear on the sample’s surface when low LED is applied, while there are lamellar structures on the sides of the samples. Cracks appear on the sample’s surface,and the splash phenomenon increases when a high LED is taken. At the same time, a large amount of unmelted powder adhered to the side of the sample. The surface quality is the best when the LED is 150-170 J/mm^(3). The preferred hatch space is currently 0.05-0.09 mm, the laser power is 200-350 W, and the average surface roughness value is(15.1±3) μm.The average surface hardness reaches HV404±HV3, higher than the forging standard range of HV340-HV395.Increasing the LED within the experiment range can increase the surface hardness, yet an excessively high LED will not further increase the surface hardness. The microstructure is composed of needle-like α’-phases with a length of about 20μm, in a crisscross ‘N’ shape, when the LED is low. The β-phase grain boundary is not obvious, and the secondaryphase volume fraction is high;when the LED is high, the α’-phase of the microstructure is in the form of coarse slats, and the secondary-phase is composed of a small amount of secondary α’-phase, the tertiary α’-phase and the fourth α’-phase disappear, and the volume fraction of the secondary-phase becomes low.展开更多
Froth flotation is a widely used process of particle separation exploiting differences in surface properties. It is important to point out that overall flotation performance(grade and recovery) is a consequence of the...Froth flotation is a widely used process of particle separation exploiting differences in surface properties. It is important to point out that overall flotation performance(grade and recovery) is a consequence of the quality and quantity of the solid particles collected from the pulp phase, transported into the froth phase, and surviving as bubble-particle aggregates into the overflow. This work will focus on studying these phenomena and will incorporate the effects of particle hydrophobicities in the 3-phase system. Solids are classed as either hydrophilic non-sulphide gangue(e.g. silica, talc), hydrophilic sulphide(e.g. pyrite), or hydrophobic sulphide(e.g. sphalerite). Talc is a surface-active species of gangue that has been shown to behave differently from silica(frother adsorbs on the surface of talc particles). Both are common components of ores and will be studied in detail. The focus of this work is to investigate the role of solids on pulp hydrodynamics, froth bubble coalescence intensity, water overflow rate with solids present, and in particular, the interactions between solids, frother and gas on the gas dispersion parameters. The results show that in the pulp zone there is no effect of solids on bubble size and gas holdup; in the froth zone, although hydrophilic particles solely do not effect on the water overflow rate, hydrophobic particles produce higher intensity of rates on water overflow and bubble coalescence, and many be attributed to the water reattachment.展开更多
The purpose of this research was to better understand the water quality status of the Xiangjiang River, China, and to evaluate the risks posed by the river water. Precisely, ten water quality parameters including p H,...The purpose of this research was to better understand the water quality status of the Xiangjiang River, China, and to evaluate the risks posed by the river water. Precisely, ten water quality parameters including p H, dissolved oxygen(DO), Escherichia coli(E. coli), potassium permanganate index(CODMn), dichromate oxidizability(CODCr), five-day biochemical oxygen demand(BOD5), ammonia nitrogen(NH4+-N), total phosphorus(TP) and fluoride(F-) as well as metal(loid)s(Pb, Hg, Cd, As, Zn, Cu and Se) were monitored monthly in 2016 at 12 sampling sites throughout the Hengyang section of the Xiangjiang River. Concentrations of all parameters were presented according to rainy and dry seasons. They were compared with Chinese surface water standards and WHO drinking water limits to assess the sustainability of the river water status. Principal component analysis(PCA) revealed different pollution sources in different seasons. Dual hierarchical cluster analysis(DHCA) was applied to further classify the water quality variables and sampling sites. Besides, a risk assessment was introduced to evaluate the carcinogenic and non-carcinogenic concerns of heavy metal(loid)s to human health. This research will help to optimize water monitoring locations and establish pollution reduction strategies on the preservation of public safety.展开更多
To provide theoretical basis and practical guidance for preparing composite rods of external diameter no larger than 12 mm by directly continuous casting,low melting point materials of lead and tin were selected to pr...To provide theoretical basis and practical guidance for preparing composite rods of external diameter no larger than 12 mm by directly continuous casting,low melting point materials of lead and tin were selected to prepare composite rods of external diameter 12 mm and inner diameter 8 mm with air pressing core filled continuous casting process.The orthogonal tests consisting of three factors and three levels were designed to investigate the parameters of melting lead temperature,continuous casting speed and air pressure that affect the performance of the composite rods.The results show that melting lead temperature is the most important factor that influences the solid/liquid interface location;continuous casting speed is the most important factor that influences the surface quality and lead and tin inter-diffusion amount;air pressure can improve the surface quality obviously and make the rods easily drawn out,but the surface quality cannot get obvious improvement when the air pressure is above 0.03 MPa.The composite rods have excellent surface quality,obvious intermediate layer,even thick clad,and metallurgical bonding interface under the condition of melting lead temperature of 375 °C,continuous casting speed of 10 mm/min,and air pressure of 0.03 MPa.展开更多
Mixed convection flow of magnetohydrodynamic(MHD) Jeffrey nanofluid over a radially stretching surface with radiative surface is studied. Radial sheet is considered to be convectively heated. Convective boundary condi...Mixed convection flow of magnetohydrodynamic(MHD) Jeffrey nanofluid over a radially stretching surface with radiative surface is studied. Radial sheet is considered to be convectively heated. Convective boundary conditions through heat and mass are employed. The governing boundary layer equations are transformed into ordinary differential equations. Convergent series solutions of the resulting problems are derived. Emphasis has been focused on studying the effects of mixed convection, thermal radiation, magnetic field and nanoparticles on the velocity, temperature and concentration fields. Numerical values of the physical parameters involved in the problem are computed for the local Nusselt and Sherwood numbers are computed.展开更多
In this study,we present the characterization of the carbon fibers recovered from the mechanochemical-enhanced recycling of carbon fiber reinforced fibers.The objectives of the study were to investigate the effect of ...In this study,we present the characterization of the carbon fibers recovered from the mechanochemical-enhanced recycling of carbon fiber reinforced fibers.The objectives of the study were to investigate the effect of our modified recycling method on the interfacial properties of recovered fibers.The reinforced plastics were recycled;the recycling efficiency was determined and the recovered fibers were sized using 1 wt%and 3 wt%concentration of(3-aminopropyl)triethoxysilane.We characterized the morphologies utilizing the electron spectroscopy for chemical analysis(ESCA),atomic force microscopy(AFM),FTIR-attenuated total reflection(ATR)spectroscopy and scanning electron microscopy(SEM).Although the surface of the fibers had no cracks,there was evidence of contaminations which affected the interfacial properties and the quality of the fibers.Results showed that the trends in the recovered and virgin fibers were similar with an increase in sizing concentration.The results highlighted the perspectives of increasing the quality of recovered fibers after the recycling process.展开更多
The contamination and environmental risk assessment of the toxic elements in sediments from the middle-downstream (Zhuzhou-Changsha section) of the Xiangjiang River in Hunan Province of China were studied. The results...The contamination and environmental risk assessment of the toxic elements in sediments from the middle-downstream (Zhuzhou-Changsha section) of the Xiangjiang River in Hunan Province of China were studied. The results show that As, Cd, Pb and Zn are major contaminants in sediments, and average concentrations of these elements significantly exceed both the Control Standards for Pollutants in Sludge of China (GB4284-84) for agricultural use in acidic soils and the effect range median (ERM) values. The average concentrations of As, Cd and Pb in the river water slightly exceed the limit of Surface Water Environment Quality Standard (GB3838-2002). The concentrations of As and Cr in depth profiles extensively change, but slight changes are observed in Pb and Zn. Cd and Zn in most sediment samples can easily enter the food-chain and bring possible ecotoxicological risk to organisms living in sediments according to the risk assessment code.展开更多
The Changsha-Xiangtan-Zhuzhou City Group is a heavy industrial district and accepted as the serious pollution area in the Xiangjiang River basin.In this study,7 metals(Pb,Hg,Cd,As,Zn,Cu and Se)and the river water qual...The Changsha-Xiangtan-Zhuzhou City Group is a heavy industrial district and accepted as the serious pollution area in the Xiangjiang River basin.In this study,7 metals(Pb,Hg,Cd,As,Zn,Cu and Se)and the river water quality parameters including pH,dissolved oxygen(DO),Escherichia coli(E.coli),potassium permanganate index(CODMn),dichromate oxidizability(CODCr),five-day biochemical oxygen demand(BOD5),ammonia nitrogen(NH4+-N),total nitrogen(TN),total phosphorus(TP)and fluoride(F)in 18 sampling sites of the Changsha-Xiangtan-Zhuzhou section are monthly monitored in 2016,which is the year to step into the second stage of the“Xiangjiang River Heavy Metal Pollution Control Implementation Plan”.It is found that E.coli,TN and TP are the main pollutants in the Changsha-Zhuzhou-Xiangtan section,and the pollution of heavy metal is not serious but As with potential risk to local people especially children should be concerned.In addition,Xiangtan city is mainly featured with heavy metal pollution,while Zhuzhou and Changsha city are both featured with other pollutants from municipal domestic sewage.展开更多
Surface tension of calcium aluminate refining slag was measured by the Slide method at 1823 K.Based on different levels of the MgO content and the mass ratio of CaO to Al_2O_3,the effects of MgO content and the mass r...Surface tension of calcium aluminate refining slag was measured by the Slide method at 1823 K.Based on different levels of the MgO content and the mass ratio of CaO to Al_2O_3,the effects of MgO content and the mass ratio of CaO to Al_2O_3 on surface tension were investigated.The results indicate that surface tension decreased with increasing MgO content(from 0 to 4.86%),followed by an increase with further increasing MgO content up to 11.33%.The trend that surface tension changed with the mass ratio of CaO to Al_2O_3 was the same as the trend that surface tension changed with the MgO content.The surface tension was varied from 0.617 N/m to 0.710 N/m,for the mass ratio of CaO to Al_2O_3 varying between 0.60 and 1.28.An attempt was made to estimate surface tension of CaO-Al_2O_3-MgO slag and its sub-system,and the application showed that the model worked well.展开更多
The normal compression tests on intact samples and artificial joints with different saw-tooth shape under cyclic loading and half-sine waves of different frequencies were performed by using Instron1342 servo-controlle...The normal compression tests on intact samples and artificial joints with different saw-tooth shape under cyclic loading and half-sine waves of different frequencies were performed by using Instron1342 servo-controlled material testing machine. The specimens were made artificially with mortar. The loading frequency ranged from 0.005 Hz to 0.1 Hz. The experimental results show that joint closure curves are non-linear and concave up. The stress-deformation curves under cyclic loading exhibit hysteresis and permanent set that diminish rapidly and keep constant finally on successive cycles. Normal displacement successively decreases from the joint J1 to J2, to J3 under the same normal loads regardless of frequency. Considering the loading frequency effect, normal displacement of joint J1 decreases with increasing the loading frequency except that the loading frequency is 0.05 Hz. Normal displacement of joint J2 increases with increasing the loading frequency. Normal displacement of joint J3 increases with increasing the loading frequency when the frequency ranges from 0.005 Hz to 0.05 Hz. Its normal displacement, however, becomes least when the loading frequency is 0.1 Hz.展开更多
Liquid nitriding of Cll0 steel was conducted in a wide range of temperatures （400-670 ℃） using a kind of chemical heat-treatments, and the hardness, mechanical and corrosion properties of the nitrided surface were ...Liquid nitriding of Cll0 steel was conducted in a wide range of temperatures （400-670 ℃） using a kind of chemical heat-treatments, and the hardness, mechanical and corrosion properties of the nitrided surface were evaluated. Experimental results revealed that the microstructure and phase constituents of the nitrided surface alloy are highly depended on the processing condition. When C 110 steel was subjected to liquid nitriding at 430 ℃, the nitrided layer was almost composed of a thin e-Fe2-3N layer. When C 110 steel was subjected to liquid nitriding at 640 ℃, the phase composition of the nitrided layer was greatly changed. The nitrided layer depth increased significantly with increasing the treating temperature. The liquid nitriding effectively improved the surface hardness. After liquid nitriding, the absorption energy of the treated sample decreased and the tensile strength increased by Charpy V-notch （CVN） test. But the elongation of treated sample decreased. The reason is that the nitrided layer of sample is hardened and there is brittlement by diffusion of nitrogen atom. Despite of treatment temperature, the liquid nitriding can improve the corrosion. After being nitrided at 430 ℃, the nitrided layer of the C110 steel was mainly composed by e-Fe2 3N, which has excellent corrosion resistance and high microhardness, the nitrided sample has the best corrosion resistance. After nitriding temperature over 580 ℃, especially at 680 ℃, the sample＇s surface was covered by the thick oxide layer, which has very low hardness and corrosion resistance. So, the corrosion resistance of samples is severely compromised.展开更多
Focusing on the issue to deal with inadequate extraction of metallogenic information especially geological information,a new method of extracting metallogenic information based on field model,i.e.the field analysis me...Focusing on the issue to deal with inadequate extraction of metallogenic information especially geological information,a new method of extracting metallogenic information based on field model,i.e.the field analysis method of metallogenic information,was proposed.In addition,a case study by using the method of the extraction of metallogenic information from the west Guangxi and southeast Yunnan district as an example was performed.The representation method for the field models of metallogenic information,including the metallogenic influence field model and the metallogenic distance field model,was discussed by introducing the concept of the field theory,based on the characteristic analysis of the distance gradualness and the influence superposition of metallogenic information.According to the field theory superposition principle and the spatial distance analysis method,the mathematical models for the metallogenic influence field and the metallogenic distance field of point,line and area geological bodies were derived out by using parameter equation and calculus.Based on the metallogenic background analysis,the metallogenic information field models of synsedimentary faults and manganese sedimentary basins were built.The relationship between the metallogenic information fields and the manganese mineralization distribution was also investigated by using the method of metallogenic information field analysis.The instance study indicates that the proposed method of metallogenic information field analysis is valid and useful for extracting the ore-controlling information of synsedimentary faults and manganese sedimentary basins in the study area,with which the extraction results are significant both statistically and geologically.展开更多
Mycoparasitic species of Trichoderma are commercially applied as biological control agents against various fungal pathogens. The mycoparasitic interaction is host specific and includes recognition, attack and subseque...Mycoparasitic species of Trichoderma are commercially applied as biological control agents against various fungal pathogens. The mycoparasitic interaction is host specific and includes recognition, attack and subsequent penetration and killing of the host. Investigations on the underlying events revealed that Trichoderma responds to multiple signals from the host (e.g. lectins or other ligands such as low molecular weight components released from the host’s cell wall) and host attack is accompanied by morphological changes and the secretion of hydrolytic enzymes and antibiotics. Degradation of the cell wall of the host fungus is-besides glucanases and proteases-mainly achieved by chitinases. In vivo studies showed that the ech42 gene (encoding endochitinase 42) is expressed before physical contact of Trichoderma with its host, probably representing one of the earliest events in mycoparasitism, whereas Nag1 (N-acetylglucosaminidase) plays a key role in the general induction of the chitinolytic enzyme system of T. atroviride . Investigations on the responsible signal transduction pathways of T. atroviride led to the isolation of several genes encoding key components of the cAMP and MAP kinase signaling pathways, as alpha and β subunits of heterotrimeric G proteins, the regulatory subunit of cAMP-dependent protein kinase, adenylate cyclase, and three MAP kinases. Analysis of knockout mutants, generated by Agrobacterium-mediated transformation, revealed that at least two alpha-subunits of heterotrimeric G proteins are participating in mycoparasitism-related signal transduction. The Tga1 G alpha subunit was shown to be involved in mycoparasitism-related processes such as chitinase expression and overproduction of toxic secondary metabolites, whereas Tga3 was found to be completely avirulent showing defects in chitinase formation and host recognition.展开更多
A novel quantitative structure-property relationship （QSPR） model for estimating the solution surface tension of 92 organic compounds at 20℃ was developed based on newly introduced atom-type topological indices. Th...A novel quantitative structure-property relationship （QSPR） model for estimating the solution surface tension of 92 organic compounds at 20℃ was developed based on newly introduced atom-type topological indices. The data set contained non-polar and polar liquids, and saturated and unsaturated compounds. The regression analysis shows that excellent result is obtained with multiple linear regression. The predictive power of the proposed model was discussed using the leave-one-out （LOO） cross-validated （CV） method. The correlation coefficient （R） and the leave-one-out cross-validation correlation coefficient （Rcv） of multiple linear regression model are 0.991 4 and 0.991 3, respectively. The new model gives the average absolute relative deviation of 1.81% for 92 substances. The result demonstrates that novel topological indices based on the equilibrium electro-negativity of atom and the relative bond length are useful model parameters for QSPR analysis of compounds.展开更多
基金湖南省教育厅基金优秀青年项目(No.22B0482)湖南科技大学博士启动基金(No.E51992 and E51993)资助。
文摘The xylitol dehydrogenase(XDH)is a crucial enzyme involved in the xylose utilization in pentose⁃catabolizing yeasts and fungi.In addition to producing xylulose,XDH can also be employed to develop a biosensor for monitoring xylitol concentration.In this study,the gene encoding the thermophilic fungus Talaromyces emersonii XDH(TeXDH)was heterologously expressed in Escherichia coli BL21(DE3)at 16℃in the soluble form.Recombinant TeXDH with high purity was purified by using a Ni⁃NTA affinity column.Size⁃exclusion chromatography and SDS⁃PAGE analysis demonstrated that the puri⁃fied recombinant TeXDH exists as a native trimer with a molecular mass of approximately 116 kD,and is composed of three identical subunits,each with a molecular weight of around 39 kD.The TeXDH strictly preferred NAD^(+)as a coenzyme to NADP^(+).The optimal temperature and pH of the TeXDH were 40℃and 10.0,respectively.After EDTA treatment,the enzyme activity of TeXDH decreased to 43.26%of the initial enzyme activity,while the divalent metal ions Mg^(2+)or Ca^(2+)could recover the enzyme activity of TeXDH,reaching 103.32%and 110.69%of the initial enzyme activity,respectively,making them the optimal divalent metal ion cofactors for TeXDH enzyme.However,the divalent metal ions of Mn^(2+),Ni^(2+),Cu^(2+),Zn^(2+),Co^(2+),and Cd^(2+)significantly inhibited the activity of TeXDH.ICP⁃MS and molecular doc⁃king studies revealed that 1 mol/L of TeXDH bound 2 mol/L Zn^(2+)ions and 1 mol/L Mg^(2+)ion.Further⁃more,TeXDH exhibited a high specificity for xylitol,laying the foundation for the development of future xylitol biosensors.
基金Projects(U23B2093,52274245)supported by the National Natural Science Foundation of ChinaProject(KFJJ22-15M)supported by the Opening Project of State Key Laboratory of Explosion Science and Technology,China。
文摘To investigate the complex macro-mechanical properties of coal from a micro-mechanical perspective,we have conducted a series of micro-mechanical experiments on coal using a nano-indentation instrument.These experiments were conducted under both dynamic and static loading conditions,allowing us to gather the micro-mechanical parameters of coal for further analysis of its micro-mechanical heterogeneity using the box counting statistical method and the Weibull model.The research findings indicate that the load–displacement curves of the coal mass under the two different loading modes exhibit noticeable discreteness.This can be attributed to the stress concentration phenomenon caused by variations in the mechanical properties of the micro-units during the loading process of the coal mass.Consequently,there are significant fluctuations in the micro-mechanical parameters of the coal mass.Moreover,the mechanical heterogeneity of the coal at the nanoscale was confirmed based on the calculation results of the standard deviation coefficient and Weibull modulus of the coal body’s micromechanical parameters.These results reveal the influence of microstructural defects and minerals on the uniformity of the stress field distribution within the loaded coal body,as well as on the ductility characteristics of the micro-defect structure.Furthermore,there is a pronounced heterogeneity in the micromechanical parameters.Furthermore,we have established a relationship between the macro and micro elastic modulus of coal by applying the Mori-Tanaka homogenization method.This relationship holds great significance for revealing the micro-mechanical failure mechanism of coal.
文摘Zinc-ion capacitors(ZICs),which consist of a capacitor-type electrode and a battery-type electrode,not only possess the high power density of supercapacitors and the high energy density of batteries,but also have other advantages such as abundant resources,high safety and environmental friendliness.However,they still face problems such as insufficient specific capacitance,a short cycling life,and narrow operating voltage and temperature ranges,which are hindering their practical use.We provide a comprehensive overview of the fundamental theory of carbon-based ZICs and summarize recent research progress from three perspectives:the carbon cathode,electrolyte and zinc anode.The influence of the structure and surface chemical properties of the carbon materials on the capacitive performance of ZICs is considered together with theoretical guidance for advancing their development and practical use.
文摘The current study comprehensively evaluates four different protein extraction methods based on urea,sodium dodecyl sulfate(SDS),anionic surfactants(BT),and total RNA extractor(Trizol),aiming to optimize the sample preparation workflow for mass spectrometry-based proteomics.Using HeLa cells as an example,we found that the method employing the mass spectrometry-compatible surfactant BT reagent significantly reduces the total time consumed for protein extraction and minimizes protein losses during the sample preparation process.Further integrating the four protein extraction methods,we identified over 7000 proteins from HeLa cells without relying on pre-fractionation techniques,and 2990 of them were quantified using label-free quantification.It is worth noting that the BT and SDS methods demonstrate higher efficiency in extracting membrane proteins,while the Urea and Trizol methods are more effective in extracting proteins from nuclear and cytoplasmic fractions.In summary,this study provides a novel solution for deep proteome coverage,particularly in the context of cellular protein extraction,by integrating mass spectrometry-compatible surfactants with traditional extraction methods to effectively enhance protein identification numbers.
基金Project(2013EG132088)supported by Special Program for Research Institutes of the Ministry of Science and Technology,ChinaProject(12010402c187)supported by Key Science and Technology Program of Anhui Province,ChinaProject(GJKJ-14-89)supported by Science and Technology Program of Nanchang Institute of Science and Technology,China
文摘Based on the working principles of particle bed comminution, particles produced by high-pressure grinding rolls （HPGR） have surface properties different from particles produced by other grinding patterns, which exert great influence on mineral flotation. Flotation performances of calcite particles under different grinding patterns involving the use of HPGR, a jaw crusher, a dry ball mill, a wet ball mill, and a wet rod mill were studied using single mineral flotation tests. The surface properties of the particles under different grinding patterns were characterized to determine the flotation performance variation in terms of specific surface area, particle size distribution, AFM, XPS, and zeta potential. The results show that particles ground by HPGR exhibited improved flotation performance within the lower range of grinding fineness in both NaOL and dodecyl amine flotation systems compared to the particles prepared using other grinding patterns. Specific surface area, particle size distribution, surface roughness, Fe（III） contamination, binding energy, and zeta potential are greatly influenced by grinding patterns, which is the main cause of the flotation performance variation.
基金Projects(51975006, 51505006) supported by the National Natural Science Foundation of China。
文摘This experiment obtained different laser energy density(LED) by changing SLM molding process parameters.The surface morphology, surface quality, and microstructure of as-fabricated samples were studied. The effects of scanning speed, hatching space, and laser power on surface quality were analyzed, and the optimal LED range for surface quality was determined. The results show that pores and spherical particles appear on the sample’s surface when low LED is applied, while there are lamellar structures on the sides of the samples. Cracks appear on the sample’s surface,and the splash phenomenon increases when a high LED is taken. At the same time, a large amount of unmelted powder adhered to the side of the sample. The surface quality is the best when the LED is 150-170 J/mm^(3). The preferred hatch space is currently 0.05-0.09 mm, the laser power is 200-350 W, and the average surface roughness value is(15.1±3) μm.The average surface hardness reaches HV404±HV3, higher than the forging standard range of HV340-HV395.Increasing the LED within the experiment range can increase the surface hardness, yet an excessively high LED will not further increase the surface hardness. The microstructure is composed of needle-like α’-phases with a length of about 20μm, in a crisscross ‘N’ shape, when the LED is low. The β-phase grain boundary is not obvious, and the secondaryphase volume fraction is high;when the LED is high, the α’-phase of the microstructure is in the form of coarse slats, and the secondary-phase is composed of a small amount of secondary α’-phase, the tertiary α’-phase and the fourth α’-phase disappear, and the volume fraction of the secondary-phase becomes low.
基金financially supported by the Chair in Mineral Processing at McGill University, under the Collaborative Research and Development program of NSERC (Natural Sciences and Engineering Research Council of Canada) with industrial sponsorship from Vale, Teck Cominco, Xstrata Process Support, Agnico-Eagle, Shell Canada, Barrick Gold, COREM, SGS Lakefield Research and Flottec
文摘Froth flotation is a widely used process of particle separation exploiting differences in surface properties. It is important to point out that overall flotation performance(grade and recovery) is a consequence of the quality and quantity of the solid particles collected from the pulp phase, transported into the froth phase, and surviving as bubble-particle aggregates into the overflow. This work will focus on studying these phenomena and will incorporate the effects of particle hydrophobicities in the 3-phase system. Solids are classed as either hydrophilic non-sulphide gangue(e.g. silica, talc), hydrophilic sulphide(e.g. pyrite), or hydrophobic sulphide(e.g. sphalerite). Talc is a surface-active species of gangue that has been shown to behave differently from silica(frother adsorbs on the surface of talc particles). Both are common components of ores and will be studied in detail. The focus of this work is to investigate the role of solids on pulp hydrodynamics, froth bubble coalescence intensity, water overflow rate with solids present, and in particular, the interactions between solids, frother and gas on the gas dispersion parameters. The results show that in the pulp zone there is no effect of solids on bubble size and gas holdup; in the froth zone, although hydrophilic particles solely do not effect on the water overflow rate, hydrophobic particles produce higher intensity of rates on water overflow and bubble coalescence, and many be attributed to the water reattachment.
基金Projects(2018YFC1801805,2018YFC1903301)supported by National Key R&D Program of ChinaProject(51825403)supported by National Science Fund for Distinguished Young Scholars,ChinaProject(2019SK2281)supported by Key R&D Program of Hunan Province,China。
文摘The purpose of this research was to better understand the water quality status of the Xiangjiang River, China, and to evaluate the risks posed by the river water. Precisely, ten water quality parameters including p H, dissolved oxygen(DO), Escherichia coli(E. coli), potassium permanganate index(CODMn), dichromate oxidizability(CODCr), five-day biochemical oxygen demand(BOD5), ammonia nitrogen(NH4+-N), total phosphorus(TP) and fluoride(F-) as well as metal(loid)s(Pb, Hg, Cd, As, Zn, Cu and Se) were monitored monthly in 2016 at 12 sampling sites throughout the Hengyang section of the Xiangjiang River. Concentrations of all parameters were presented according to rainy and dry seasons. They were compared with Chinese surface water standards and WHO drinking water limits to assess the sustainability of the river water status. Principal component analysis(PCA) revealed different pollution sources in different seasons. Dual hierarchical cluster analysis(DHCA) was applied to further classify the water quality variables and sampling sites. Besides, a risk assessment was introduced to evaluate the carcinogenic and non-carcinogenic concerns of heavy metal(loid)s to human health. This research will help to optimize water monitoring locations and establish pollution reduction strategies on the preservation of public safety.
基金Project(2009AA03Z532) supported by the National High Technology Research and Development Program of ChinaProject(50774009) supported by the National Natural Science Foundation of China
文摘To provide theoretical basis and practical guidance for preparing composite rods of external diameter no larger than 12 mm by directly continuous casting,low melting point materials of lead and tin were selected to prepare composite rods of external diameter 12 mm and inner diameter 8 mm with air pressing core filled continuous casting process.The orthogonal tests consisting of three factors and three levels were designed to investigate the parameters of melting lead temperature,continuous casting speed and air pressure that affect the performance of the composite rods.The results show that melting lead temperature is the most important factor that influences the solid/liquid interface location;continuous casting speed is the most important factor that influences the surface quality and lead and tin inter-diffusion amount;air pressure can improve the surface quality obviously and make the rods easily drawn out,but the surface quality cannot get obvious improvement when the air pressure is above 0.03 MPa.The composite rods have excellent surface quality,obvious intermediate layer,even thick clad,and metallurgical bonding interface under the condition of melting lead temperature of 375 °C,continuous casting speed of 10 mm/min,and air pressure of 0.03 MPa.
文摘Mixed convection flow of magnetohydrodynamic(MHD) Jeffrey nanofluid over a radially stretching surface with radiative surface is studied. Radial sheet is considered to be convectively heated. Convective boundary conditions through heat and mass are employed. The governing boundary layer equations are transformed into ordinary differential equations. Convergent series solutions of the resulting problems are derived. Emphasis has been focused on studying the effects of mixed convection, thermal radiation, magnetic field and nanoparticles on the velocity, temperature and concentration fields. Numerical values of the physical parameters involved in the problem are computed for the local Nusselt and Sherwood numbers are computed.
基金Project(S2598445)supported by the Project for Cooperative R&D between Industry,Academy and Research Institute Funded by the Korea Ministry of SME and Startups in 2018
文摘In this study,we present the characterization of the carbon fibers recovered from the mechanochemical-enhanced recycling of carbon fiber reinforced fibers.The objectives of the study were to investigate the effect of our modified recycling method on the interfacial properties of recovered fibers.The reinforced plastics were recycled;the recycling efficiency was determined and the recovered fibers were sized using 1 wt%and 3 wt%concentration of(3-aminopropyl)triethoxysilane.We characterized the morphologies utilizing the electron spectroscopy for chemical analysis(ESCA),atomic force microscopy(AFM),FTIR-attenuated total reflection(ATR)spectroscopy and scanning electron microscopy(SEM).Although the surface of the fibers had no cracks,there was evidence of contaminations which affected the interfacial properties and the quality of the fibers.Results showed that the trends in the recovered and virgin fibers were similar with an increase in sizing concentration.The results highlighted the perspectives of increasing the quality of recovered fibers after the recycling process.
基金Project (20507022) supported by the National Natural Science Foundation of ChinaProject (EREH050303) supported by the Foundation of Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health
文摘The contamination and environmental risk assessment of the toxic elements in sediments from the middle-downstream (Zhuzhou-Changsha section) of the Xiangjiang River in Hunan Province of China were studied. The results show that As, Cd, Pb and Zn are major contaminants in sediments, and average concentrations of these elements significantly exceed both the Control Standards for Pollutants in Sludge of China (GB4284-84) for agricultural use in acidic soils and the effect range median (ERM) values. The average concentrations of As, Cd and Pb in the river water slightly exceed the limit of Surface Water Environment Quality Standard (GB3838-2002). The concentrations of As and Cr in depth profiles extensively change, but slight changes are observed in Pb and Zn. Cd and Zn in most sediment samples can easily enter the food-chain and bring possible ecotoxicological risk to organisms living in sediments according to the risk assessment code.
基金Projects(2018YFC1903301,2018YFC1801805)supported by the National Key R&D Program of China
文摘The Changsha-Xiangtan-Zhuzhou City Group is a heavy industrial district and accepted as the serious pollution area in the Xiangjiang River basin.In this study,7 metals(Pb,Hg,Cd,As,Zn,Cu and Se)and the river water quality parameters including pH,dissolved oxygen(DO),Escherichia coli(E.coli),potassium permanganate index(CODMn),dichromate oxidizability(CODCr),five-day biochemical oxygen demand(BOD5),ammonia nitrogen(NH4+-N),total nitrogen(TN),total phosphorus(TP)and fluoride(F)in 18 sampling sites of the Changsha-Xiangtan-Zhuzhou section are monthly monitored in 2016,which is the year to step into the second stage of the“Xiangjiang River Heavy Metal Pollution Control Implementation Plan”.It is found that E.coli,TN and TP are the main pollutants in the Changsha-Zhuzhou-Xiangtan section,and the pollution of heavy metal is not serious but As with potential risk to local people especially children should be concerned.In addition,Xiangtan city is mainly featured with heavy metal pollution,while Zhuzhou and Changsha city are both featured with other pollutants from municipal domestic sewage.
基金Project(51204115)supported by the National Natural Science Foundation of ChinaProject(BK20130308)supported by the Basic Research Program of Jiangsu Province,ChinaProject(2014M561710)supported by China Postdoctoral Science Foundation
文摘Surface tension of calcium aluminate refining slag was measured by the Slide method at 1823 K.Based on different levels of the MgO content and the mass ratio of CaO to Al_2O_3,the effects of MgO content and the mass ratio of CaO to Al_2O_3 on surface tension were investigated.The results indicate that surface tension decreased with increasing MgO content(from 0 to 4.86%),followed by an increase with further increasing MgO content up to 11.33%.The trend that surface tension changed with the mass ratio of CaO to Al_2O_3 was the same as the trend that surface tension changed with the MgO content.The surface tension was varied from 0.617 N/m to 0.710 N/m,for the mass ratio of CaO to Al_2O_3 varying between 0.60 and 1.28.An attempt was made to estimate surface tension of CaO-Al_2O_3-MgO slag and its sub-system,and the application showed that the model worked well.
基金Projects(50490274 50490272) supported by the National Natural Science Foundation of ChinaProject(2002CB412703) supported by theNational Basic Research Program of China
文摘The normal compression tests on intact samples and artificial joints with different saw-tooth shape under cyclic loading and half-sine waves of different frequencies were performed by using Instron1342 servo-controlled material testing machine. The specimens were made artificially with mortar. The loading frequency ranged from 0.005 Hz to 0.1 Hz. The experimental results show that joint closure curves are non-linear and concave up. The stress-deformation curves under cyclic loading exhibit hysteresis and permanent set that diminish rapidly and keep constant finally on successive cycles. Normal displacement successively decreases from the joint J1 to J2, to J3 under the same normal loads regardless of frequency. Considering the loading frequency effect, normal displacement of joint J1 decreases with increasing the loading frequency except that the loading frequency is 0.05 Hz. Normal displacement of joint J2 increases with increasing the loading frequency. Normal displacement of joint J3 increases with increasing the loading frequency when the frequency ranges from 0.005 Hz to 0.05 Hz. Its normal displacement, however, becomes least when the loading frequency is 0.1 Hz.
基金Projects(51471112,51611130204)supported by the National Natural Science Foundation of China
文摘Liquid nitriding of Cll0 steel was conducted in a wide range of temperatures （400-670 ℃） using a kind of chemical heat-treatments, and the hardness, mechanical and corrosion properties of the nitrided surface were evaluated. Experimental results revealed that the microstructure and phase constituents of the nitrided surface alloy are highly depended on the processing condition. When C 110 steel was subjected to liquid nitriding at 430 ℃, the nitrided layer was almost composed of a thin e-Fe2-3N layer. When C 110 steel was subjected to liquid nitriding at 640 ℃, the phase composition of the nitrided layer was greatly changed. The nitrided layer depth increased significantly with increasing the treating temperature. The liquid nitriding effectively improved the surface hardness. After liquid nitriding, the absorption energy of the treated sample decreased and the tensile strength increased by Charpy V-notch （CVN） test. But the elongation of treated sample decreased. The reason is that the nitrided layer of sample is hardened and there is brittlement by diffusion of nitrogen atom. Despite of treatment temperature, the liquid nitriding can improve the corrosion. After being nitrided at 430 ℃, the nitrided layer of the C110 steel was mainly composed by e-Fe2 3N, which has excellent corrosion resistance and high microhardness, the nitrided sample has the best corrosion resistance. After nitriding temperature over 580 ℃, especially at 680 ℃, the sample＇s surface was covered by the thick oxide layer, which has very low hardness and corrosion resistance. So, the corrosion resistance of samples is severely compromised.
基金Project(2006BAB01B07) supported by the National Science and Technology Pillar Program during the 11th Five-Year Plan Period of China
文摘Focusing on the issue to deal with inadequate extraction of metallogenic information especially geological information,a new method of extracting metallogenic information based on field model,i.e.the field analysis method of metallogenic information,was proposed.In addition,a case study by using the method of the extraction of metallogenic information from the west Guangxi and southeast Yunnan district as an example was performed.The representation method for the field models of metallogenic information,including the metallogenic influence field model and the metallogenic distance field model,was discussed by introducing the concept of the field theory,based on the characteristic analysis of the distance gradualness and the influence superposition of metallogenic information.According to the field theory superposition principle and the spatial distance analysis method,the mathematical models for the metallogenic influence field and the metallogenic distance field of point,line and area geological bodies were derived out by using parameter equation and calculus.Based on the metallogenic background analysis,the metallogenic information field models of synsedimentary faults and manganese sedimentary basins were built.The relationship between the metallogenic information fields and the manganese mineralization distribution was also investigated by using the method of metallogenic information field analysis.The instance study indicates that the proposed method of metallogenic information field analysis is valid and useful for extracting the ore-controlling information of synsedimentary faults and manganese sedimentary basins in the study area,with which the extraction results are significant both statistically and geologically.
文摘Mycoparasitic species of Trichoderma are commercially applied as biological control agents against various fungal pathogens. The mycoparasitic interaction is host specific and includes recognition, attack and subsequent penetration and killing of the host. Investigations on the underlying events revealed that Trichoderma responds to multiple signals from the host (e.g. lectins or other ligands such as low molecular weight components released from the host’s cell wall) and host attack is accompanied by morphological changes and the secretion of hydrolytic enzymes and antibiotics. Degradation of the cell wall of the host fungus is-besides glucanases and proteases-mainly achieved by chitinases. In vivo studies showed that the ech42 gene (encoding endochitinase 42) is expressed before physical contact of Trichoderma with its host, probably representing one of the earliest events in mycoparasitism, whereas Nag1 (N-acetylglucosaminidase) plays a key role in the general induction of the chitinolytic enzyme system of T. atroviride . Investigations on the responsible signal transduction pathways of T. atroviride led to the isolation of several genes encoding key components of the cAMP and MAP kinase signaling pathways, as alpha and β subunits of heterotrimeric G proteins, the regulatory subunit of cAMP-dependent protein kinase, adenylate cyclase, and three MAP kinases. Analysis of knockout mutants, generated by Agrobacterium-mediated transformation, revealed that at least two alpha-subunits of heterotrimeric G proteins are participating in mycoparasitism-related signal transduction. The Tga1 G alpha subunit was shown to be involved in mycoparasitism-related processes such as chitinase expression and overproduction of toxic secondary metabolites, whereas Tga3 was found to be completely avirulent showing defects in chitinase formation and host recognition.
基金Projects(20775010,21075011) supported by the National Natural Science Foundation of ChinaProject(2008AA05Z405) supported by the National High Technology Research and Development Program of China+2 种基金Project(09JJ3016) supported by Hunan Provincial Natural Science Foundation,ChinaProject(09C066) supported by Scientific Research Fund of Hunan Provincial Education Department,ChinaProject(2010CL01) supported by the Foundation of Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation,China
文摘A novel quantitative structure-property relationship （QSPR） model for estimating the solution surface tension of 92 organic compounds at 20℃ was developed based on newly introduced atom-type topological indices. The data set contained non-polar and polar liquids, and saturated and unsaturated compounds. The regression analysis shows that excellent result is obtained with multiple linear regression. The predictive power of the proposed model was discussed using the leave-one-out （LOO） cross-validated （CV） method. The correlation coefficient （R） and the leave-one-out cross-validation correlation coefficient （Rcv） of multiple linear regression model are 0.991 4 and 0.991 3, respectively. The new model gives the average absolute relative deviation of 1.81% for 92 substances. The result demonstrates that novel topological indices based on the equilibrium electro-negativity of atom and the relative bond length are useful model parameters for QSPR analysis of compounds.
