从多肽序列的一级结构出发,基于多肽序列中氨基酸侧链间距离和氨基酸侧链的电性特征,构建了多肽序列特征矢量,简称ζ矢量,选取文献中主要组织相容性复合物(MHC)中的14个A^d限制性和14个非A^d限制性多肽序列作为训练集建立辅助性T细胞(he...从多肽序列的一级结构出发,基于多肽序列中氨基酸侧链间距离和氨基酸侧链的电性特征,构建了多肽序列特征矢量,简称ζ矢量,选取文献中主要组织相容性复合物(MHC)中的14个A^d限制性和14个非A^d限制性多肽序列作为训练集建立辅助性T细胞(helper T lymphocytc,Th)表位预测的定量模型,为了检验该预报系统的精确性,进行了随机抽样检验和交互检验。结果表明,该预报系统具有稳定性好、预测能力强的特点,该方法可用于人的MHC Ⅰ和Ⅱ类表位的预测、蛋白质抗原免疫识别、亚单位疫苗分子设计及研制。展开更多
Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte li...Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte lines（HaCaT cells） were treated with different doses of curcumin.The effects of curcumin on cell viability were measured by MTT assay,and the cell cycle distribution and apoptosis determined by flow cytometry.The mRNA expression changes of proliferating cell nuclear antigen（PCNA）,cyclin D1 and Bcl-xL were from real-time PCR analysis and the protein levels were detected by Western blotting.Results：Data obtained in the study showed that curcumin could cause significantly inhibitory effect on proliferation in HaCaT cells in a time- and dose-dependent manner.Cell arrest at G1/S phase and significant apoptosis were observed after being treated with curcumin for 24 h.In association with these,the expression of PCNA,cyclin D1 and Bcl-xL were decreased both at mRNA and protein levels for the same treatment.Conclusion：Curcumin can inhibit proliferation,induce cell arrest at G1/S phase and cause apoptosis in HaCaT cells.The decreased expression of PCNA,cyclin D1 and Bcl-xL induced by curcumin contributes to the above effects in vitro.展开更多
文摘从多肽序列的一级结构出发,基于多肽序列中氨基酸侧链间距离和氨基酸侧链的电性特征,构建了多肽序列特征矢量,简称ζ矢量,选取文献中主要组织相容性复合物(MHC)中的14个A^d限制性和14个非A^d限制性多肽序列作为训练集建立辅助性T细胞(helper T lymphocytc,Th)表位预测的定量模型,为了检验该预报系统的精确性,进行了随机抽样检验和交互检验。结果表明,该预报系统具有稳定性好、预测能力强的特点,该方法可用于人的MHC Ⅰ和Ⅱ类表位的预测、蛋白质抗原免疫识别、亚单位疫苗分子设计及研制。
文摘Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte lines（HaCaT cells） were treated with different doses of curcumin.The effects of curcumin on cell viability were measured by MTT assay,and the cell cycle distribution and apoptosis determined by flow cytometry.The mRNA expression changes of proliferating cell nuclear antigen（PCNA）,cyclin D1 and Bcl-xL were from real-time PCR analysis and the protein levels were detected by Western blotting.Results：Data obtained in the study showed that curcumin could cause significantly inhibitory effect on proliferation in HaCaT cells in a time- and dose-dependent manner.Cell arrest at G1/S phase and significant apoptosis were observed after being treated with curcumin for 24 h.In association with these,the expression of PCNA,cyclin D1 and Bcl-xL were decreased both at mRNA and protein levels for the same treatment.Conclusion：Curcumin can inhibit proliferation,induce cell arrest at G1/S phase and cause apoptosis in HaCaT cells.The decreased expression of PCNA,cyclin D1 and Bcl-xL induced by curcumin contributes to the above effects in vitro.
