p-dimethylaminobenzonitrile (DMABN), a typical dual fluorescent flurophor (TICTflurophore), was used as fluorescent probe. The microenviromental property of twelve methyl modified β-cyclodextrin(methyl β-CD) was com...p-dimethylaminobenzonitrile (DMABN), a typical dual fluorescent flurophor (TICTflurophore), was used as fluorescent probe. The microenviromental property of twelve methyl modified β-cyclodextrin(methyl β-CD) was compared with that of β-cyclodextrin (β-CD). Results showed that the polarity of the cavity of methyl β-CD is lower than that of β- CD, and the capacity of methyl β-CD inclusion with DMABN is stronger than that of β-CD inclusion with DMABN.展开更多
荧光定量PCR技术不仅实现了PCR从定性到定量的飞跃,更以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为分子生物学研究的重要工具,并用于生物特异核苷酸的检测。主要概述了荧光染料法、TaqMan探针法、分子...荧光定量PCR技术不仅实现了PCR从定性到定量的飞跃,更以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为分子生物学研究的重要工具,并用于生物特异核苷酸的检测。主要概述了荧光染料法、TaqMan探针法、分子信标法、Lux Primer 4种荧光定量技术的原理、特点以及荧光定量PCR技术在各方面的应用。展开更多
文摘p-dimethylaminobenzonitrile (DMABN), a typical dual fluorescent flurophor (TICTflurophore), was used as fluorescent probe. The microenviromental property of twelve methyl modified β-cyclodextrin(methyl β-CD) was compared with that of β-cyclodextrin (β-CD). Results showed that the polarity of the cavity of methyl β-CD is lower than that of β- CD, and the capacity of methyl β-CD inclusion with DMABN is stronger than that of β-CD inclusion with DMABN.
文摘荧光定量PCR技术不仅实现了PCR从定性到定量的飞跃,更以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为分子生物学研究的重要工具,并用于生物特异核苷酸的检测。主要概述了荧光染料法、TaqMan探针法、分子信标法、Lux Primer 4种荧光定量技术的原理、特点以及荧光定量PCR技术在各方面的应用。
