The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave ...The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper,the long-term stability of the PPESO3 and the PPESO3 mixed with trypsin was studied,and the detection of proteolytic cleavage of bovine serum albumin(BSA) was obtained satisfactory results. The PPESO3 was provided a novel,simple,quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile,the fluorescent detection devices can be integrated into the microwave assisted protein digestion system,for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.展开更多
文摘The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper,the long-term stability of the PPESO3 and the PPESO3 mixed with trypsin was studied,and the detection of proteolytic cleavage of bovine serum albumin(BSA) was obtained satisfactory results. The PPESO3 was provided a novel,simple,quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile,the fluorescent detection devices can be integrated into the microwave assisted protein digestion system,for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.
