目的:选择羟基积雪草苷和依克多因为原料,制备一种具有舒缓功效的脂质体乳剂,并评价其透皮效果。方法:细胞实验探究能产生抗炎活性和屏障修复活性的羟基积雪草苷和依克多因的最佳配比;薄膜分散法制备羟基积雪草苷-依克多因脂质体;马尔...目的:选择羟基积雪草苷和依克多因为原料,制备一种具有舒缓功效的脂质体乳剂,并评价其透皮效果。方法:细胞实验探究能产生抗炎活性和屏障修复活性的羟基积雪草苷和依克多因的最佳配比;薄膜分散法制备羟基积雪草苷-依克多因脂质体;马尔文粒径仪测定其粒径、分散系数及电位;透射电镜下观察其形态;高效液相色谱法测定溶液中羟基积雪草苷及依克多因含量;透析法测定包封率和载药量;Franz扩散池测定脂质体的透皮情况。结果:细胞实验确定了羟基积雪草苷和依克多因按照质量比1∶9配比,其抗炎活性和屏障修复活性最佳。药物在此配比下,再按照磷胆比5∶1、药脂比1∶10条件下制备得到脂质体,该脂质体羟基积雪草苷包封率为51.33%、羟基积雪草苷载药量0.35%、依克多因包封率26.39%、依克多因载药量1.20%;粒径为166.53 nm,电位为-29.63 m V,PDI为0.20,24 h内累积透过量低于水溶液,无明显突释效应,具有缓释效果。结论:本实验制备的脂质体质量佳,内部颗粒分布均一较为稳定,同时可以达到在皮肤表面的缓释效果,延长功效物质的作用时间。展开更多
It aims to investigate the protective effects of sodium hyaluronate,panthenol,Portulaca oleracea L.and Calendula officinalis L.on hyperosmotic dehydration-induced injury of human immortalized keratinocytes(HaCaT).The ...It aims to investigate the protective effects of sodium hyaluronate,panthenol,Portulaca oleracea L.and Calendula officinalis L.on hyperosmotic dehydration-induced injury of human immortalized keratinocytes(HaCaT).The safety mass concentrations of four raw materials were screened by detecting cell viability,and the secretion of hyaluronic acid(HA)was determined using the ELISA method.The expression of HaCaT barrier function related genes(OVOL1,EREG,TGM1,TGM2,IVL,IRF6,THBS1,CASP14)was detected at the mRNA level to explore the regulatory effect of four raw materials on these genes.The results demonstrate that pretreatment with the four kinds of raw materials could increase the cell viability after hyperosmotic dehydration,promote the secretion of HA,and improve the expression of barrier function related genes after hyperosmotic dehydration,among which panthenol and Calendula officinalis L.are better.The results show that the four raw materials have a certain protective effect on the hyperosmotic dehydration cell model,which provides data support for its application in cosmetics.展开更多
文摘目的:选择羟基积雪草苷和依克多因为原料,制备一种具有舒缓功效的脂质体乳剂,并评价其透皮效果。方法:细胞实验探究能产生抗炎活性和屏障修复活性的羟基积雪草苷和依克多因的最佳配比;薄膜分散法制备羟基积雪草苷-依克多因脂质体;马尔文粒径仪测定其粒径、分散系数及电位;透射电镜下观察其形态;高效液相色谱法测定溶液中羟基积雪草苷及依克多因含量;透析法测定包封率和载药量;Franz扩散池测定脂质体的透皮情况。结果:细胞实验确定了羟基积雪草苷和依克多因按照质量比1∶9配比,其抗炎活性和屏障修复活性最佳。药物在此配比下,再按照磷胆比5∶1、药脂比1∶10条件下制备得到脂质体,该脂质体羟基积雪草苷包封率为51.33%、羟基积雪草苷载药量0.35%、依克多因包封率26.39%、依克多因载药量1.20%;粒径为166.53 nm,电位为-29.63 m V,PDI为0.20,24 h内累积透过量低于水溶液,无明显突释效应,具有缓释效果。结论:本实验制备的脂质体质量佳,内部颗粒分布均一较为稳定,同时可以达到在皮肤表面的缓释效果,延长功效物质的作用时间。
文摘It aims to investigate the protective effects of sodium hyaluronate,panthenol,Portulaca oleracea L.and Calendula officinalis L.on hyperosmotic dehydration-induced injury of human immortalized keratinocytes(HaCaT).The safety mass concentrations of four raw materials were screened by detecting cell viability,and the secretion of hyaluronic acid(HA)was determined using the ELISA method.The expression of HaCaT barrier function related genes(OVOL1,EREG,TGM1,TGM2,IVL,IRF6,THBS1,CASP14)was detected at the mRNA level to explore the regulatory effect of four raw materials on these genes.The results demonstrate that pretreatment with the four kinds of raw materials could increase the cell viability after hyperosmotic dehydration,promote the secretion of HA,and improve the expression of barrier function related genes after hyperosmotic dehydration,among which panthenol and Calendula officinalis L.are better.The results show that the four raw materials have a certain protective effect on the hyperosmotic dehydration cell model,which provides data support for its application in cosmetics.
