A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to ...A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10 -6 to 2.4×10 -3 mol/L with detection limit of 1.0×10 -6 mol/L. The apparent Michaelis-Menten constant K m is found to be 3.2×10 -3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol -1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.展开更多
An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and hor...An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO 2 nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.展开更多
文摘A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10 -6 to 2.4×10 -3 mol/L with detection limit of 1.0×10 -6 mol/L. The apparent Michaelis-Menten constant K m is found to be 3.2×10 -3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol -1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.
文摘An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO 2 nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.
