The interaction of oxidized FAD radical with dGMP, dAMP and single-stranded DNA (ssDNA) was investigated in neutral aqueous solution using time-resolved 248 nm laser flash photolysis aimed at elucidation of the initia...The interaction of oxidized FAD radical with dGMP, dAMP and single-stranded DNA (ssDNA) was investigated in neutral aqueous solution using time-resolved 248 nm laser flash photolysis aimed at elucidation of the initial photosensitization mechanism. The characterized absorption spectra of transient species were observed. Moreover, direct observation of stabilized DNA guanyl radical has provided transient evidence for site-selective photosensitized damage of DNA at guanine moiety, which has simulated the major initial species produced by the direct effect of ionizing radiation or UV light on DNA.展开更多
文摘目的 探究代谢相关脂肪性肝病(MAFLD)患者血清几丁质酶样蛋白40(YKL-40)、烟酰胺腺嘌呤二核苷酸磷酸氧化酶2(NOX2)表达水平,并分析其与肝纤维化的关系。方法 选取2020年4月至2021年12月于保定市人民医院就诊的108例MAFLD患者为MAFLD组,选取同期该院108例健康体检者为对照组。肝纤维化程度评估根据瞬时弹性成像技术所得肝脏硬度值分为非纤维化组(60例,肝脏硬度值<8.0 k Pa)和纤维化组(48例,肝脏硬度值≥8.0 k Pa)。比较研究对象YKL-40、NOX2及临床资料差异。logistic回归分析MAFLD患者发生肝纤维化的影响因素。受试者工作特征(ROC)曲线评价YKL-40、NOX2对肝纤维化的预测效能。结果 MAFLD组血清YKL-40、NOX2水平高于对照组,差异有统计学意义(P<0.05)。纤维化组YKL-40、NOX2水平高于非纤维化组,差异有统计学意义(P<0.05)。回归分析显示,年龄(OR=1.647,95%CI:1.053~2.575,P=0.029)、HOMA-IR(OR=1.758,95%CI:1.083~2.853,P=0.022)、YKL-40(OR=2.016,95%CI:1.237~3.284,P=0.004)、NOX2(OR=2.292,95%CI:1.388~3.786,P=0.001)是MAFLD患者发生肝纤维化的影响因素(P<0.05)。YKL-40、NOX2单独预测MAFLD患者肝纤维化的曲线下面积(AUC)分别为0.833、0.838,YKL-40联合NOX2预测MAFLD患者肝纤维化的AUC为0.922,优于单一指标(Z_(二者联合-YKL-40)=2.268,P=0.023、Z_(二者联合-NOX2)=1.999,P=0.046)。结论 YKL-40、NOX2在MAFLD患者血清中水平增加,且与肝纤维化相关,YKL-40联合NOX2可作为预测肝纤维化的生物标志物。
文摘The interaction of oxidized FAD radical with dGMP, dAMP and single-stranded DNA (ssDNA) was investigated in neutral aqueous solution using time-resolved 248 nm laser flash photolysis aimed at elucidation of the initial photosensitization mechanism. The characterized absorption spectra of transient species were observed. Moreover, direct observation of stabilized DNA guanyl radical has provided transient evidence for site-selective photosensitized damage of DNA at guanine moiety, which has simulated the major initial species produced by the direct effect of ionizing radiation or UV light on DNA.
