A bacterium B2 isolated from the Tianshan glacier of Xinjiang could produce blue pigments.According to 16S rDNA analysis, this isolate belonged to Duganella Genus .Two compounds were separated and purified from the cu...A bacterium B2 isolated from the Tianshan glacier of Xinjiang could produce blue pigments.According to 16S rDNA analysis, this isolate belonged to Duganella Genus .Two compounds were separated and purified from the cultivated Duganella B2 , named Blue-Ⅰand Blue-Ⅱ, respectively.From the spectra data of UV, MS and NMR of the compounds, Blue-Ⅰwas confirmed to be deoxyviolacein and Blue-Ⅱ was violacein.Blue-Ⅰand Blue-Ⅱ had the respective molecular weights of 327.2 and 343.2,and showed the characteristic absorption peaks at the respective 560 and 572 nm within the visible light range in ethanol solution.These results will be useful for developing the bioprocess for producing bacterial violacein.展开更多
基于组氨酸的结构基础,以82.7~85.2%的产率合成了4种N-酰基高丝氨酸内酯(AHL)的结构类似物(His-7,His-8,His-10和His-12),经IR、1H NMR、13 C NMR和MS确认了产物的结构。将四种化合物与紫色色杆菌共培养考察其对革兰氏阴性细菌群体感应...基于组氨酸的结构基础,以82.7~85.2%的产率合成了4种N-酰基高丝氨酸内酯(AHL)的结构类似物(His-7,His-8,His-10和His-12),经IR、1H NMR、13 C NMR和MS确认了产物的结构。将四种化合物与紫色色杆菌共培养考察其对革兰氏阴性细菌群体感应系统的影响。结果表明:His-7对紫色色杆菌群体感应系统具有增强信号的作用,能够显著促进菌体中脱氧紫色杆菌素(DVio)的表达。随着分子中脂肪链长度的增加,AHL结构类似物对DVio的表达表现了抑制作用。展开更多
文摘A bacterium B2 isolated from the Tianshan glacier of Xinjiang could produce blue pigments.According to 16S rDNA analysis, this isolate belonged to Duganella Genus .Two compounds were separated and purified from the cultivated Duganella B2 , named Blue-Ⅰand Blue-Ⅱ, respectively.From the spectra data of UV, MS and NMR of the compounds, Blue-Ⅰwas confirmed to be deoxyviolacein and Blue-Ⅱ was violacein.Blue-Ⅰand Blue-Ⅱ had the respective molecular weights of 327.2 and 343.2,and showed the characteristic absorption peaks at the respective 560 and 572 nm within the visible light range in ethanol solution.These results will be useful for developing the bioprocess for producing bacterial violacein.
文摘基于组氨酸的结构基础,以82.7~85.2%的产率合成了4种N-酰基高丝氨酸内酯(AHL)的结构类似物(His-7,His-8,His-10和His-12),经IR、1H NMR、13 C NMR和MS确认了产物的结构。将四种化合物与紫色色杆菌共培养考察其对革兰氏阴性细菌群体感应系统的影响。结果表明:His-7对紫色色杆菌群体感应系统具有增强信号的作用,能够显著促进菌体中脱氧紫色杆菌素(DVio)的表达。随着分子中脂肪链长度的增加,AHL结构类似物对DVio的表达表现了抑制作用。
