Objectives.To study the expression of TNF α mRNA and the effect of somatostatin on the expression of TNF α mRNA in multiorgans of rats with acute hemorrhagic necrotic pancreatitis(AHNP). Methods.AHNP in the rat was ...Objectives.To study the expression of TNF α mRNA and the effect of somatostatin on the expression of TNF α mRNA in multiorgans of rats with acute hemorrhagic necrotic pancreatitis(AHNP). Methods.AHNP in the rat was induced by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. Somatostatin octapeptide (SS-OP) (2μg/kg)was injected into the femoral vein imme- diately in rats of the treatment group after inductive AHNP. Rats of the sham operative group received in- jection of saline. Sixty animals of the AHNP and sham operative groups at the designated time(0. 2h, 0. 5 h, 2h, 4h, 8h, after the operation,six animals at each time point)and tweleve animals of treatment group at 4h after the operation were sacrificed for samples of pancreas, liver and lung. The expressions of TNF α mRNA within the pancreas, liver and lung were established by RT－PCR. Results. TNF α mRNA became detectable in the pancreas as early as 0. 2h after inductive AHNP, while it was undetectable in other organs until 0. 5h. Expression of TNF α mRNA in each tissue increased continuously and reached a peak at 4h,demonstrating a significant difference compared with that at 0. 5h and 8h. Expressions of TNF α mRNA from pancreas, liver and lung were decreased 50-80% in the treat- ment group, the pancreatic necrosis was also attenuated dramatically. Conclusion. TNF α mRNA was detectable in pancreas,liver and lung tissues at the early stage of AH- NP.SS-OP can significantly inhibit the expression of TNF α mRNA and attenuate the pancreatic necrosis. We feel that this may be an important mechanism of SS-OP in the treatment of AHNP.展开更多
Retinoic acid receptor-α (RAR α) plays a major role in the growth inhibitory effect of retinoic acid on human breast cancer cells, may be it could serve as an indicator to guide the treatment and prevent of breast c...Retinoic acid receptor-α (RAR α) plays a major role in the growth inhibitory effect of retinoic acid on human breast cancer cells, may be it could serve as an indicator to guide the treatment and prevent of breast cancer with retinoic acid in clinic. All previous researchs were based on observing the changes of RAR α mRAN expression. In this study, the expression of RAR α in human breast cell lines was studied by Northern Blot, Western Blot and Immunohistochemistry in mRNA level and protein level. Results showed that RAR α protein expression was correlated with RAR α mRNA expression. RAR α mRNA ex- pression was higher in estrogen receptor (ER)-positive human breast cancer cell lines than in ER-negative ones. So was RAR a protein expression. Both RAR α mRNA amd RAR α protein expression were associ- ated with ER status. The expression of RAR α and the relationship between RAR a and ER status were al- so determined by immunohistochemistry in 58 human primary breast cancer tumors. 37 (63. 8% ) tumors were ER-positive and of these 28 (75. 7% ) were also RAR α -positive. The coexpression of ER and RAR a was statistically significant (P＜0. 01, by X^2 contingency analysis). It was reported that RAR α expres- sion in cultured breast cancer cells was regulated by estrogen acting via the ER. Our study demonstrated that RAR α expression may be modulated in breast cancer in vivo by estrogen via ER.展开更多
文摘Objectives.To study the expression of TNF α mRNA and the effect of somatostatin on the expression of TNF α mRNA in multiorgans of rats with acute hemorrhagic necrotic pancreatitis(AHNP). Methods.AHNP in the rat was induced by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. Somatostatin octapeptide (SS-OP) (2μg/kg)was injected into the femoral vein imme- diately in rats of the treatment group after inductive AHNP. Rats of the sham operative group received in- jection of saline. Sixty animals of the AHNP and sham operative groups at the designated time(0. 2h, 0. 5 h, 2h, 4h, 8h, after the operation,six animals at each time point)and tweleve animals of treatment group at 4h after the operation were sacrificed for samples of pancreas, liver and lung. The expressions of TNF α mRNA within the pancreas, liver and lung were established by RT－PCR. Results. TNF α mRNA became detectable in the pancreas as early as 0. 2h after inductive AHNP, while it was undetectable in other organs until 0. 5h. Expression of TNF α mRNA in each tissue increased continuously and reached a peak at 4h,demonstrating a significant difference compared with that at 0. 5h and 8h. Expressions of TNF α mRNA from pancreas, liver and lung were decreased 50-80% in the treat- ment group, the pancreatic necrosis was also attenuated dramatically. Conclusion. TNF α mRNA was detectable in pancreas,liver and lung tissues at the early stage of AH- NP.SS-OP can significantly inhibit the expression of TNF α mRNA and attenuate the pancreatic necrosis. We feel that this may be an important mechanism of SS-OP in the treatment of AHNP.
文摘Retinoic acid receptor-α (RAR α) plays a major role in the growth inhibitory effect of retinoic acid on human breast cancer cells, may be it could serve as an indicator to guide the treatment and prevent of breast cancer with retinoic acid in clinic. All previous researchs were based on observing the changes of RAR α mRAN expression. In this study, the expression of RAR α in human breast cell lines was studied by Northern Blot, Western Blot and Immunohistochemistry in mRNA level and protein level. Results showed that RAR α protein expression was correlated with RAR α mRNA expression. RAR α mRNA ex- pression was higher in estrogen receptor (ER)-positive human breast cancer cell lines than in ER-negative ones. So was RAR a protein expression. Both RAR α mRNA amd RAR α protein expression were associ- ated with ER status. The expression of RAR α and the relationship between RAR a and ER status were al- so determined by immunohistochemistry in 58 human primary breast cancer tumors. 37 (63. 8% ) tumors were ER-positive and of these 28 (75. 7% ) were also RAR α -positive. The coexpression of ER and RAR a was statistically significant (P＜0. 01, by X^2 contingency analysis). It was reported that RAR α expres- sion in cultured breast cancer cells was regulated by estrogen acting via the ER. Our study demonstrated that RAR α expression may be modulated in breast cancer in vivo by estrogen via ER.
