The primary Syrian hamster embryo(SHE) cells were used to study the oncogenic transformation by ￣(238)pu α particles or X-rays alone or in combination with a chemical promoter phorbol ester.Survival curves of SHE ce...The primary Syrian hamster embryo(SHE) cells were used to study the oncogenic transformation by ￣(238)pu α particles or X-rays alone or in combination with a chemical promoter phorbol ester.Survival curves of SHE cells following exposure to α-particles or X-rays were fitted to single-or multi-target models,respectively. Model parameters were: Do = 0. 55 Gy. n = 1 for α particles 4 Do = 1.44 Gy. Dq = 3.0 Gy. n=7.7 for X-rays.Incidence of α particles or X-rays induced cell transformation was dose-dependant.α particles were more efficient in inducing cell transformation than that of X-rays. The enhancement of SHE cell transformation by phorbol 12-myristate 13-acetate(PMA) following exposure to α particles of 0. 25-1. 00 Gy was observed.展开更多
Objective: To establish a patient-like human ovary carcinoma /spontaneous metastasis model using orthotopic transplanation of histologically intact tumor tissue. Methods: An highly metastatic ovarian tumor line (HO-89...Objective: To establish a patient-like human ovary carcinoma /spontaneous metastasis model using orthotopic transplanation of histologically intact tumor tissue. Methods: An highly metastatic ovarian tumor line (HO-8910PM: Human serum carcinoma of the ovary )previously grown substaneously was transplanted into the ovicapsule using microsurgery technique .Histologically intact human ovary tumor pieces gained from implantation site were passaged between ovicapsules for four generations. Results: All mice developed ovary tumors and the metastatic rates were about 75%. The tumors only metastasized to liver but no other organs. The earliest appearance of metastasis was 14 d and the average survival period was 20.7±4.89 d.The microscopic appearance of the metastases was similar to the tumor observed in the substaneous xenografts and orthotopically transplanted. Chromosomes analysis exhibited the feature of human carcinoma and retained genetic stability during the processes of passage. Conclusion: Orthotopic implanation provides a suitable micro-enviroment in which ovarian cancer can express its intrinsic clinically-relevant properties. This approach is relevant to the spontaneous development of ovarian cancer and is thought to be a useful model for studies of metastatic mechanism and therapy for ovary cancer.展开更多
文摘The primary Syrian hamster embryo(SHE) cells were used to study the oncogenic transformation by ￣(238)pu α particles or X-rays alone or in combination with a chemical promoter phorbol ester.Survival curves of SHE cells following exposure to α-particles or X-rays were fitted to single-or multi-target models,respectively. Model parameters were: Do = 0. 55 Gy. n = 1 for α particles 4 Do = 1.44 Gy. Dq = 3.0 Gy. n=7.7 for X-rays.Incidence of α particles or X-rays induced cell transformation was dose-dependant.α particles were more efficient in inducing cell transformation than that of X-rays. The enhancement of SHE cell transformation by phorbol 12-myristate 13-acetate(PMA) following exposure to α particles of 0. 25-1. 00 Gy was observed.
文摘Objective: To establish a patient-like human ovary carcinoma /spontaneous metastasis model using orthotopic transplanation of histologically intact tumor tissue. Methods: An highly metastatic ovarian tumor line (HO-8910PM: Human serum carcinoma of the ovary )previously grown substaneously was transplanted into the ovicapsule using microsurgery technique .Histologically intact human ovary tumor pieces gained from implantation site were passaged between ovicapsules for four generations. Results: All mice developed ovary tumors and the metastatic rates were about 75%. The tumors only metastasized to liver but no other organs. The earliest appearance of metastasis was 14 d and the average survival period was 20.7±4.89 d.The microscopic appearance of the metastases was similar to the tumor observed in the substaneous xenografts and orthotopically transplanted. Chromosomes analysis exhibited the feature of human carcinoma and retained genetic stability during the processes of passage. Conclusion: Orthotopic implanation provides a suitable micro-enviroment in which ovarian cancer can express its intrinsic clinically-relevant properties. This approach is relevant to the spontaneous development of ovarian cancer and is thought to be a useful model for studies of metastatic mechanism and therapy for ovary cancer.
