Objective:To explore the protective effect of remifentanil on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury and their possible mechanism. Methods:The model of rat hepatic ischemia-reperfusio...Objective:To explore the protective effect of remifentanil on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury and their possible mechanism. Methods:The model of rat hepatic ischemia-reperfusion injury was used and the effect of remifentanil on the ultrastructure of mitochondria, calcium homeostasis, MDA level in mitochondria were observed. Results: In contrast with the control group, mitochondrial matrix calcium concentration, calcium concentration after calcium uptake, and the quantity of calcium uptake in low and high remifentanil concentration groups and 5-HD group are lower (P<0. 01), and there is no difference in RHD (5-HD+remifentanil) group. The difference in MDA level between groups is insignificant. Conclusion:Remifentanil at clinical concentrations exerts a protective effect on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury, in which activating the KATP channel may be involved.展开更多
To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepatic VX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by u...To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepatic VX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by using a non-traumatic vascular clamp to block the branches distributing in the left-middle lobe of the hepatic artery for 60 min, and subsequently the clamp was removed and the reperfusion injury of hepatic neoplasm occurred. At different time-points, the normal and hepatic neoplasm tissues of the animal models were taken out to detect the superoxide dismutase (SOD) and malondialdehyde (MDA) respectively.The results show that the products and injurious effects of oxygen free radical (OFR) of the neoplasm tissues are more serious than those of the normal hepatic tissues.展开更多
The present study was to investigate changes of free radicals in the whole rat liver, changes of ATP levels of hepatic cells, ultrastructural changes in hepatic tissue during ischemia and reperfusion in rats and the ...The present study was to investigate changes of free radicals in the whole rat liver, changes of ATP levels of hepatic cells, ultrastructural changes in hepatic tissue during ischemia and reperfusion in rats and the effects of ligustrazine and salvia compound. The results indicated that : 1 ) the free radical levels increased by 8. 3-fold and 9.0-fold in the groups with 30 and 60 min ischemia after 40 min reperfusion, as compared with the group with ischemia only (P<0.01, P<0.01, respectively). 2) The levels of ATP returned to normal in the group with 30 min ischemia after reperfusion for 40 min and 3 d , remained low in the group with 90 min ischemia and fell again after a mild increase in the group with 60 min ischemia. 3) The hepatic and endothelial cell damage after ischemia became more severe after reperfusion, as revealed by electron microscopy. The presenet study also showed that ligustrazine and salvia compound have protective effects against reperfusion injury. They can be used to scavenge free radicals, improve hepatic microcirculation and alleviate hepatic and endothelial cell damage.展开更多
文摘Objective:To explore the protective effect of remifentanil on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury and their possible mechanism. Methods:The model of rat hepatic ischemia-reperfusion injury was used and the effect of remifentanil on the ultrastructure of mitochondria, calcium homeostasis, MDA level in mitochondria were observed. Results: In contrast with the control group, mitochondrial matrix calcium concentration, calcium concentration after calcium uptake, and the quantity of calcium uptake in low and high remifentanil concentration groups and 5-HD group are lower (P<0. 01), and there is no difference in RHD (5-HD+remifentanil) group. The difference in MDA level between groups is insignificant. Conclusion:Remifentanil at clinical concentrations exerts a protective effect on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury, in which activating the KATP channel may be involved.
文摘To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepatic VX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by using a non-traumatic vascular clamp to block the branches distributing in the left-middle lobe of the hepatic artery for 60 min, and subsequently the clamp was removed and the reperfusion injury of hepatic neoplasm occurred. At different time-points, the normal and hepatic neoplasm tissues of the animal models were taken out to detect the superoxide dismutase (SOD) and malondialdehyde (MDA) respectively.The results show that the products and injurious effects of oxygen free radical (OFR) of the neoplasm tissues are more serious than those of the normal hepatic tissues.
文摘The present study was to investigate changes of free radicals in the whole rat liver, changes of ATP levels of hepatic cells, ultrastructural changes in hepatic tissue during ischemia and reperfusion in rats and the effects of ligustrazine and salvia compound. The results indicated that : 1 ) the free radical levels increased by 8. 3-fold and 9.0-fold in the groups with 30 and 60 min ischemia after 40 min reperfusion, as compared with the group with ischemia only (P<0.01, P<0.01, respectively). 2) The levels of ATP returned to normal in the group with 30 min ischemia after reperfusion for 40 min and 3 d , remained low in the group with 90 min ischemia and fell again after a mild increase in the group with 60 min ischemia. 3) The hepatic and endothelial cell damage after ischemia became more severe after reperfusion, as revealed by electron microscopy. The presenet study also showed that ligustrazine and salvia compound have protective effects against reperfusion injury. They can be used to scavenge free radicals, improve hepatic microcirculation and alleviate hepatic and endothelial cell damage.
