Fatty liver fibrosis, a severe type of Nonalcoholic fatty liver disease （NAFLD）, can be reversed by effec- tive medical intervention. Endoplasmic reticulum stress promotes liver fat accumulation and induction of in...Fatty liver fibrosis, a severe type of Nonalcoholic fatty liver disease （NAFLD）, can be reversed by effec- tive medical intervention. Endoplasmic reticulum stress promotes liver fat accumulation and induction of inflammatory cytokines, which plays an important role in the process of liver fibrosis. Our previous study have shown that, SPJ （saponins of Panax Japonicus） has significant antioxidant and anti-inflammatory activity. Therefore, the protective effects and mechanisms of SPJ against fatty liver fibrosis in mice were investigated. In this study, fatty liver fibrosis was induced by high-fat diet combined with intraperitoneal injection of heterologous protein. The results showed that SPJ significantly improved liver function and decreased the serum lipid level. SPJ remarkly decreased the liver steato- sis, collagen fibers and inflammatory cell infiltration. Furthermore, treatment with SPJ significantly downregulated the mRNA level of Collagen Type 1α （COLL 1α）, α-SMA, TIMP, CHOP and GRP78. In addition, SPJ significantly de- creased the protein level of p-JNK, COLL 1α and GRP78. Taken together, SPJ protect against fatty liver fibrosis through inhibition of endoplasmic reticulum stress response and CHOP and JNK-mediated apoptosis and inflammation pathway. These results indicate that SPJ might be a potential drug for prevention of liver fibrosis.展开更多
文摘Fatty liver fibrosis, a severe type of Nonalcoholic fatty liver disease （NAFLD）, can be reversed by effec- tive medical intervention. Endoplasmic reticulum stress promotes liver fat accumulation and induction of inflammatory cytokines, which plays an important role in the process of liver fibrosis. Our previous study have shown that, SPJ （saponins of Panax Japonicus） has significant antioxidant and anti-inflammatory activity. Therefore, the protective effects and mechanisms of SPJ against fatty liver fibrosis in mice were investigated. In this study, fatty liver fibrosis was induced by high-fat diet combined with intraperitoneal injection of heterologous protein. The results showed that SPJ significantly improved liver function and decreased the serum lipid level. SPJ remarkly decreased the liver steato- sis, collagen fibers and inflammatory cell infiltration. Furthermore, treatment with SPJ significantly downregulated the mRNA level of Collagen Type 1α （COLL 1α）, α-SMA, TIMP, CHOP and GRP78. In addition, SPJ significantly de- creased the protein level of p-JNK, COLL 1α and GRP78. Taken together, SPJ protect against fatty liver fibrosis through inhibition of endoplasmic reticulum stress response and CHOP and JNK-mediated apoptosis and inflammation pathway. These results indicate that SPJ might be a potential drug for prevention of liver fibrosis.
