A method to release L-asparaginase（EC3.5.1.1） from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether （POOP...A method to release L-asparaginase（EC3.5.1.1） from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether （POOPE）. This method combined enzyme release with enzyme purification.The influences of POOPE concentration, K2HPO4 concentration,E. coli cel l concentration,pH and treatment time on the release of L-asparaginase were inv estigated.Under suitable conditions,over 90% of L-asparaginase in cells was re leased into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner str ucture of E.coli cells significantly.展开更多
文摘A method to release L-asparaginase（EC3.5.1.1） from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether （POOPE）. This method combined enzyme release with enzyme purification.The influences of POOPE concentration, K2HPO4 concentration,E. coli cel l concentration,pH and treatment time on the release of L-asparaginase were inv estigated.Under suitable conditions,over 90% of L-asparaginase in cells was re leased into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner str ucture of E.coli cells significantly.
