A novel chiral stationary phase(CSP) for HPLC was prepared by synthesizing 3,5-dinitrobenzoyl-(S)-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid as chiral selector and bonding it with 3-aminopropylsilane modified si...A novel chiral stationary phase(CSP) for HPLC was prepared by synthesizing 3,5-dinitrobenzoyl-(S)-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid as chiral selector and bonding it with 3-aminopropylsilane modified silica.The resolutions of 8 enantiomers of binaphthol and its derivatives were achieved by using hexane-ethanol-acetic acid(98∶2∶0.5,volume ratio) as mobile phase with a column temperature 30 ℃ and detected at ultraviolet 254 nm.The effects of acetic acid and alcohol organic modifiers on retention and resolutions of the analytes were examined.The enantioselectivities α of the analytes on CSP could be improved by adding acetic acid to mobile phase due to masking effect of H^+ on the residing silanol and amino group.展开更多
文摘A novel chiral stationary phase(CSP) for HPLC was prepared by synthesizing 3,5-dinitrobenzoyl-(S)-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid as chiral selector and bonding it with 3-aminopropylsilane modified silica.The resolutions of 8 enantiomers of binaphthol and its derivatives were achieved by using hexane-ethanol-acetic acid(98∶2∶0.5,volume ratio) as mobile phase with a column temperature 30 ℃ and detected at ultraviolet 254 nm.The effects of acetic acid and alcohol organic modifiers on retention and resolutions of the analytes were examined.The enantioselectivities α of the analytes on CSP could be improved by adding acetic acid to mobile phase due to masking effect of H^+ on the residing silanol and amino group.
