构建具备良好热稳定性、自组装性质及生物相容性的可食性细胞外基质(extracellular matrix,ECM)类似物支架对于制造结构化细胞培养肉制品至关重要。将羧甲基壳聚糖(carboxymethyl chitosan,CMCS)引入牛骨胶原蛋白(bovine bone collagen,...构建具备良好热稳定性、自组装性质及生物相容性的可食性细胞外基质(extracellular matrix,ECM)类似物支架对于制造结构化细胞培养肉制品至关重要。将羧甲基壳聚糖(carboxymethyl chitosan,CMCS)引入牛骨胶原蛋白(bovine bone collagen,BBC)体系中,通过光谱分析(紫外、红外、荧光光谱)发现BBC与CMCS的相互作用随着引入CMCS添加量的增加而增强,但并未影响BBC的三螺旋结构。差示扫描量热法/热重分析结果表明,CMCS的引入增强了BBC体系的热稳定性。浊度试验及扫描电子显微镜/透射电子显微镜观察结果证实了CMCS引入后胶原蛋白纤维形成度呈上升趋势,聚集行为更明显且自组装速率产生变化,呈现出更疏松扭曲的三维结构以及更大的纤维直径及更广泛的直径分布。但CMCS的引入并未明显影响BBC的D-周期性结构(胶原纤维自组装过程中形成的特征性明暗交替的周期性横纹结构)形成及其长度,且CMCS引入前后体系的细胞相容性也未呈现显著性差异。随着引入CMCS添加量增加,CMCS和BBC之间的静电作用力可能较共价相互作用和氢键更占优势。这些结果表明,CMCS的引入不影响BBC三螺旋结构完整性和生物相容性,并改善了BBC的热稳定性及体外自组装性质。这为开发新型优良可食性胶原蛋白基ECM仿生支架在细胞培养肉领域的应用以及畜禽骨副产物高值化精深加工利用提供了参考信息。展开更多
The adsorption isotherm and adsorption reaction kinetics of carboxymethyl chitosan(CMCS) for Fe 2+ have been studied. The results showed that CMCS appeared a good adsorbent for Fe 2+ in solution with pH 6~8 and the ad...The adsorption isotherm and adsorption reaction kinetics of carboxymethyl chitosan(CMCS) for Fe 2+ have been studied. The results showed that CMCS appeared a good adsorbent for Fe 2+ in solution with pH 6~8 and the adsorption capacity of CMCS increased with the increase in its carboxyl groups. The adsorption isotherm of CMCS for Fe 2+ at 20 ℃ was fitted good for the Langmuir equation.展开更多
INTENTION:The effect of different reaction conditions such as temperature,pH value,time,the concentration of Na+ ions and Pb2+ ions on the complexation capacity of carboxymethyl chitosan（CMCS） to Ca2+ions,were inves...INTENTION:The effect of different reaction conditions such as temperature,pH value,time,the concentration of Na+ ions and Pb2+ ions on the complexation capacity of carboxymethyl chitosan（CMCS） to Ca2+ions,were investigated.METHOD:In different reaction conditions,carboxymethyl chitosan reacted with saturated solution of calcirm chorde,produced carboxymethyl chitoxan calcium（CCC）.The Ca2+ions concentration of CCC was determined by atomic absorption spectrophotometer.RESULT:After 15 minutes,the complexation capacity of Ca2+ions to CMCS was to most in the room temperature at pH6.0.CONCLUSION:The complexation of Ca2+ions to CMCS has been influenced by the complexation capacity of CMCS to other cations in the reactions system.展开更多
文摘构建具备良好热稳定性、自组装性质及生物相容性的可食性细胞外基质(extracellular matrix,ECM)类似物支架对于制造结构化细胞培养肉制品至关重要。将羧甲基壳聚糖(carboxymethyl chitosan,CMCS)引入牛骨胶原蛋白(bovine bone collagen,BBC)体系中,通过光谱分析(紫外、红外、荧光光谱)发现BBC与CMCS的相互作用随着引入CMCS添加量的增加而增强,但并未影响BBC的三螺旋结构。差示扫描量热法/热重分析结果表明,CMCS的引入增强了BBC体系的热稳定性。浊度试验及扫描电子显微镜/透射电子显微镜观察结果证实了CMCS引入后胶原蛋白纤维形成度呈上升趋势,聚集行为更明显且自组装速率产生变化,呈现出更疏松扭曲的三维结构以及更大的纤维直径及更广泛的直径分布。但CMCS的引入并未明显影响BBC的D-周期性结构(胶原纤维自组装过程中形成的特征性明暗交替的周期性横纹结构)形成及其长度,且CMCS引入前后体系的细胞相容性也未呈现显著性差异。随着引入CMCS添加量增加,CMCS和BBC之间的静电作用力可能较共价相互作用和氢键更占优势。这些结果表明,CMCS的引入不影响BBC三螺旋结构完整性和生物相容性,并改善了BBC的热稳定性及体外自组装性质。这为开发新型优良可食性胶原蛋白基ECM仿生支架在细胞培养肉领域的应用以及畜禽骨副产物高值化精深加工利用提供了参考信息。
文摘The adsorption isotherm and adsorption reaction kinetics of carboxymethyl chitosan(CMCS) for Fe 2+ have been studied. The results showed that CMCS appeared a good adsorbent for Fe 2+ in solution with pH 6~8 and the adsorption capacity of CMCS increased with the increase in its carboxyl groups. The adsorption isotherm of CMCS for Fe 2+ at 20 ℃ was fitted good for the Langmuir equation.
文摘INTENTION:The effect of different reaction conditions such as temperature,pH value,time,the concentration of Na+ ions and Pb2+ ions on the complexation capacity of carboxymethyl chitosan（CMCS） to Ca2+ions,were investigated.METHOD:In different reaction conditions,carboxymethyl chitosan reacted with saturated solution of calcirm chorde,produced carboxymethyl chitoxan calcium（CCC）.The Ca2+ions concentration of CCC was determined by atomic absorption spectrophotometer.RESULT:After 15 minutes,the complexation capacity of Ca2+ions to CMCS was to most in the room temperature at pH6.0.CONCLUSION:The complexation of Ca2+ions to CMCS has been influenced by the complexation capacity of CMCS to other cations in the reactions system.
