The 276 pair-primers （nuclear and chloroplast microsatellite） developed from seven species of Pinaceae were selected and identified for cross-species transferability to ten Pinus species （P massoniana, P kesiya, P ...The 276 pair-primers （nuclear and chloroplast microsatellite） developed from seven species of Pinaceae were selected and identified for cross-species transferability to ten Pinus species （P massoniana, P kesiya, P tabulaeformis P densiflora, P thunbergii, P caribaea, P taeda, P yunnanensis, P densata, P sylvestris）belonging to Sect. Pinus by BSA （bulked segregate analysis） method. The results showed that 23 of 276 （8.0%） markers were successful to have amplification product in ten species, and 5 of 23 （21.7%） were polymorphic cross species and lack of polymorphic within species. Eight of 10 Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species （P. kesiya and P. densata）.展开更多
A decision-making model of gear process for green manufacturing is presented, which integrates the five objectives including the time, quality, cost, resource consumption and environmental impact of gear process toget...A decision-making model of gear process for green manufacturing is presented, which integrates the five objectives including the time, quality, cost, resource consumption and environmental impact of gear process together into the development of a strategy. Mathematical description is provided for the multi-objectives decision-making model. The expert judgment and the multi-fuzzy assessment theory are introduced to do sensible comparisons and give quantitative results. A case study on practical cutting tool selection in gear machining demonstrates that the proposed model is applicable.展开更多
文摘The 276 pair-primers （nuclear and chloroplast microsatellite） developed from seven species of Pinaceae were selected and identified for cross-species transferability to ten Pinus species （P massoniana, P kesiya, P tabulaeformis P densiflora, P thunbergii, P caribaea, P taeda, P yunnanensis, P densata, P sylvestris）belonging to Sect. Pinus by BSA （bulked segregate analysis） method. The results showed that 23 of 276 （8.0%） markers were successful to have amplification product in ten species, and 5 of 23 （21.7%） were polymorphic cross species and lack of polymorphic within species. Eight of 10 Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species （P. kesiya and P. densata）.
文摘A decision-making model of gear process for green manufacturing is presented, which integrates the five objectives including the time, quality, cost, resource consumption and environmental impact of gear process together into the development of a strategy. Mathematical description is provided for the multi-objectives decision-making model. The expert judgment and the multi-fuzzy assessment theory are introduced to do sensible comparisons and give quantitative results. A case study on practical cutting tool selection in gear machining demonstrates that the proposed model is applicable.
