s 107 strains of bacteria was isolated from crop,stomach and intestine of the Bullacta exarata and 85% of them were Gram_negative rod. The microflora of digestive tract was composed of eleven genera. The predominant g...s 107 strains of bacteria was isolated from crop,stomach and intestine of the Bullacta exarata and 85% of them were Gram_negative rod. The microflora of digestive tract was composed of eleven genera. The predominant genera were composed of Photobacterium , Vibrio , Bacillus, and Pseudomonas. The results indicate that predominant microflora was relatively steady in digestive tract of Bullacta exarata growth period. There were a great variety of bacteria in crop. The digestive tract may play a special role for regulating and controlling the microbial community.展开更多
该研究采用Illumina Mi Seq高通量测序技术对采集自张家界市的7个酸鱼样品进行了细菌类群分析,结果发现厚壁菌门(Firmicutes)为优势菌门,其平均相对含量为95.49%。优势细菌属为乳酸杆菌属(Lactobacillus)、四联球菌属(Tetragenococcus)...该研究采用Illumina Mi Seq高通量测序技术对采集自张家界市的7个酸鱼样品进行了细菌类群分析,结果发现厚壁菌门(Firmicutes)为优势菌门,其平均相对含量为95.49%。优势细菌属为乳酸杆菌属(Lactobacillus)、四联球菌属(Tetragenococcus)和葡萄球菌属(Staphylococcus),其平均相对含量分别为66.53%、11.77%和8.10%。发现8个核心分类操作单元中有6个注释为Lactobacillus,各有1个注释为Tetragenococcus和Staphylococcus,其中乳酸菌的累计平均相对含量达60.27%。PICRUSt技术预测酸鱼菌群功能结果表明,酸鱼中的细菌菌群在"氨基酸转运与代谢"和"碳水化合物运输和代谢"功能上表达较高,说明菌群在代谢方面的功能比较突出。由此可见,张家界地区酸鱼中蕴含了丰富的乳酸菌资源,且以Lactobacillus、Tetragenococcus和Lactococcus为主。展开更多
Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the ...Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.展开更多
文摘s 107 strains of bacteria was isolated from crop,stomach and intestine of the Bullacta exarata and 85% of them were Gram_negative rod. The microflora of digestive tract was composed of eleven genera. The predominant genera were composed of Photobacterium , Vibrio , Bacillus, and Pseudomonas. The results indicate that predominant microflora was relatively steady in digestive tract of Bullacta exarata growth period. There were a great variety of bacteria in crop. The digestive tract may play a special role for regulating and controlling the microbial community.
文摘该研究采用Illumina Mi Seq高通量测序技术对采集自张家界市的7个酸鱼样品进行了细菌类群分析,结果发现厚壁菌门(Firmicutes)为优势菌门,其平均相对含量为95.49%。优势细菌属为乳酸杆菌属(Lactobacillus)、四联球菌属(Tetragenococcus)和葡萄球菌属(Staphylococcus),其平均相对含量分别为66.53%、11.77%和8.10%。发现8个核心分类操作单元中有6个注释为Lactobacillus,各有1个注释为Tetragenococcus和Staphylococcus,其中乳酸菌的累计平均相对含量达60.27%。PICRUSt技术预测酸鱼菌群功能结果表明,酸鱼中的细菌菌群在"氨基酸转运与代谢"和"碳水化合物运输和代谢"功能上表达较高,说明菌群在代谢方面的功能比较突出。由此可见,张家界地区酸鱼中蕴含了丰富的乳酸菌资源,且以Lactobacillus、Tetragenococcus和Lactococcus为主。
文摘Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.
